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An Antibiotic-Loaded Silicone–Hydrogel Interpenetrating Polymer Network for the Prevention of Surgical Site Infections

Surgical site infections (SSIs) are among the most frequent healthcare-associated infections, resulting in high morbidity, mortality, and cost. While correct hygiene measures and prophylactic antibiotics are effective in preventing SSIs, even in modern healthcare settings where recommended guideline...

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Autores principales: Grønnemose, Rasmus Birkholm, Tornby, Ditte Rask, Riber, Sara Schødt, Hjelmager, Janni Søvsø, Riber, Lars Peter Schødt, Lindholt, Jes Sanddal, Andersen, Thomas Emil
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10606314/
https://www.ncbi.nlm.nih.gov/pubmed/37888399
http://dx.doi.org/10.3390/gels9100826
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author Grønnemose, Rasmus Birkholm
Tornby, Ditte Rask
Riber, Sara Schødt
Hjelmager, Janni Søvsø
Riber, Lars Peter Schødt
Lindholt, Jes Sanddal
Andersen, Thomas Emil
author_facet Grønnemose, Rasmus Birkholm
Tornby, Ditte Rask
Riber, Sara Schødt
Hjelmager, Janni Søvsø
Riber, Lars Peter Schødt
Lindholt, Jes Sanddal
Andersen, Thomas Emil
author_sort Grønnemose, Rasmus Birkholm
collection PubMed
description Surgical site infections (SSIs) are among the most frequent healthcare-associated infections, resulting in high morbidity, mortality, and cost. While correct hygiene measures and prophylactic antibiotics are effective in preventing SSIs, even in modern healthcare settings where recommended guidelines are strictly followed, SSIs persist as a considerable problem that has proven hard to solve. Surgical procedures involving the implantation of foreign bodies are particularly problematic due to the ability of microorganisms to adhere to and colonize the implanted material and form resilient biofilms. In these cases, SSIs may develop even months after implantation and can be difficult to treat once established. Locally applied antibiotics or specifically engineered implant materials with built-in antibiotic-release properties may prevent these complications and, ultimately, require fewer antibiotics compared to those that are systemically administered. In this study, we demonstrated an antimicrobial material concept with intended use in artificial vascular grafts. The material is a silicone–hydrogel interpenetrating polymer network developed earlier for drug-release catheters. In this study, we designed the material for permanent implantation and tested the drug-loading and drug-release properties of the material to prevent the growth of a typical causative pathogen of SSIs, Staphylococcus aureus. The novelty of this study is demonstrated through the antimicrobial properties of the material in vitro after loading it with an advantageous combination, minocycline and rifampicin, which subsequently showed superiority over the state-of-the-art (Propaten) artificial graft material in a large-animal study, using a novel porcine tissue-implantation model.
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spelling pubmed-106063142023-10-28 An Antibiotic-Loaded Silicone–Hydrogel Interpenetrating Polymer Network for the Prevention of Surgical Site Infections Grønnemose, Rasmus Birkholm Tornby, Ditte Rask Riber, Sara Schødt Hjelmager, Janni Søvsø Riber, Lars Peter Schødt Lindholt, Jes Sanddal Andersen, Thomas Emil Gels Article Surgical site infections (SSIs) are among the most frequent healthcare-associated infections, resulting in high morbidity, mortality, and cost. While correct hygiene measures and prophylactic antibiotics are effective in preventing SSIs, even in modern healthcare settings where recommended guidelines are strictly followed, SSIs persist as a considerable problem that has proven hard to solve. Surgical procedures involving the implantation of foreign bodies are particularly problematic due to the ability of microorganisms to adhere to and colonize the implanted material and form resilient biofilms. In these cases, SSIs may develop even months after implantation and can be difficult to treat once established. Locally applied antibiotics or specifically engineered implant materials with built-in antibiotic-release properties may prevent these complications and, ultimately, require fewer antibiotics compared to those that are systemically administered. In this study, we demonstrated an antimicrobial material concept with intended use in artificial vascular grafts. The material is a silicone–hydrogel interpenetrating polymer network developed earlier for drug-release catheters. In this study, we designed the material for permanent implantation and tested the drug-loading and drug-release properties of the material to prevent the growth of a typical causative pathogen of SSIs, Staphylococcus aureus. The novelty of this study is demonstrated through the antimicrobial properties of the material in vitro after loading it with an advantageous combination, minocycline and rifampicin, which subsequently showed superiority over the state-of-the-art (Propaten) artificial graft material in a large-animal study, using a novel porcine tissue-implantation model. MDPI 2023-10-19 /pmc/articles/PMC10606314/ /pubmed/37888399 http://dx.doi.org/10.3390/gels9100826 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Grønnemose, Rasmus Birkholm
Tornby, Ditte Rask
Riber, Sara Schødt
Hjelmager, Janni Søvsø
Riber, Lars Peter Schødt
Lindholt, Jes Sanddal
Andersen, Thomas Emil
An Antibiotic-Loaded Silicone–Hydrogel Interpenetrating Polymer Network for the Prevention of Surgical Site Infections
title An Antibiotic-Loaded Silicone–Hydrogel Interpenetrating Polymer Network for the Prevention of Surgical Site Infections
title_full An Antibiotic-Loaded Silicone–Hydrogel Interpenetrating Polymer Network for the Prevention of Surgical Site Infections
title_fullStr An Antibiotic-Loaded Silicone–Hydrogel Interpenetrating Polymer Network for the Prevention of Surgical Site Infections
title_full_unstemmed An Antibiotic-Loaded Silicone–Hydrogel Interpenetrating Polymer Network for the Prevention of Surgical Site Infections
title_short An Antibiotic-Loaded Silicone–Hydrogel Interpenetrating Polymer Network for the Prevention of Surgical Site Infections
title_sort antibiotic-loaded silicone–hydrogel interpenetrating polymer network for the prevention of surgical site infections
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10606314/
https://www.ncbi.nlm.nih.gov/pubmed/37888399
http://dx.doi.org/10.3390/gels9100826
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