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Effect Sizes of Cognitive and Locomotive Behavior Tests in the 5XFAD-J Mouse Model of Alzheimer’s Disease

Alzheimer’s disease (AD) is characterized by the accumulation of amyloid β (Aβ) plaques in the brain, leading to cognitive impairment and other clinical symptoms. The 5XFAD mouse model is commonly used in AD research because it expresses five human transgenes that result in the accumulation of Aβ pl...

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Autores principales: Choi, Moonseok, Jang, Hyung-Sup, Son, Taekwon, Kim, Dongsoo, Youn, Young-Jin, Hwang, Gyu-Bin, Choi, Young Pyo, Jeong, Yun Ha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10606448/
https://www.ncbi.nlm.nih.gov/pubmed/37894744
http://dx.doi.org/10.3390/ijms242015064
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author Choi, Moonseok
Jang, Hyung-Sup
Son, Taekwon
Kim, Dongsoo
Youn, Young-Jin
Hwang, Gyu-Bin
Choi, Young Pyo
Jeong, Yun Ha
author_facet Choi, Moonseok
Jang, Hyung-Sup
Son, Taekwon
Kim, Dongsoo
Youn, Young-Jin
Hwang, Gyu-Bin
Choi, Young Pyo
Jeong, Yun Ha
author_sort Choi, Moonseok
collection PubMed
description Alzheimer’s disease (AD) is characterized by the accumulation of amyloid β (Aβ) plaques in the brain, leading to cognitive impairment and other clinical symptoms. The 5XFAD mouse model is commonly used in AD research because it expresses five human transgenes that result in the accumulation of Aβ plaques and cognitive decline at a relatively early age. Behavioral experiments are frequently conducted using this model; however, the effect size has not yet been reported. In this study, we examined basic cognition and locomotion in 5XFAD mice with a C57BL6/J background (5XFAD-J) at 6 months of age, a period in which impairments of cognitive function and locomotion are commonly observed. We analyzed the effect sizes of cognitive and locomotive experiments in the 5XFAD mice compared with those in the wild-type mice. Our results suggest that for long-term memory analysis, the novel object recognition test (p = 0.013, effect size 1.24) required a sample size of at least 12 to obtain meaningful results. Moreover, analysis of general locomotion over total distance with the Laboratory Animal Behavior Observation, Registration and Analysis System (LABORAS) test during the dark phase (p = 0.007, effect size −1.37) needed a sample size of 10 for a statistical power (1-β) of 0.8. In conclusion, we can conduct more ethical and scientifically rigorous animal experiments using 5XFAD mice based on the effect and sample sizes suggested in this study.
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spelling pubmed-106064482023-10-28 Effect Sizes of Cognitive and Locomotive Behavior Tests in the 5XFAD-J Mouse Model of Alzheimer’s Disease Choi, Moonseok Jang, Hyung-Sup Son, Taekwon Kim, Dongsoo Youn, Young-Jin Hwang, Gyu-Bin Choi, Young Pyo Jeong, Yun Ha Int J Mol Sci Brief Report Alzheimer’s disease (AD) is characterized by the accumulation of amyloid β (Aβ) plaques in the brain, leading to cognitive impairment and other clinical symptoms. The 5XFAD mouse model is commonly used in AD research because it expresses five human transgenes that result in the accumulation of Aβ plaques and cognitive decline at a relatively early age. Behavioral experiments are frequently conducted using this model; however, the effect size has not yet been reported. In this study, we examined basic cognition and locomotion in 5XFAD mice with a C57BL6/J background (5XFAD-J) at 6 months of age, a period in which impairments of cognitive function and locomotion are commonly observed. We analyzed the effect sizes of cognitive and locomotive experiments in the 5XFAD mice compared with those in the wild-type mice. Our results suggest that for long-term memory analysis, the novel object recognition test (p = 0.013, effect size 1.24) required a sample size of at least 12 to obtain meaningful results. Moreover, analysis of general locomotion over total distance with the Laboratory Animal Behavior Observation, Registration and Analysis System (LABORAS) test during the dark phase (p = 0.007, effect size −1.37) needed a sample size of 10 for a statistical power (1-β) of 0.8. In conclusion, we can conduct more ethical and scientifically rigorous animal experiments using 5XFAD mice based on the effect and sample sizes suggested in this study. MDPI 2023-10-11 /pmc/articles/PMC10606448/ /pubmed/37894744 http://dx.doi.org/10.3390/ijms242015064 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Brief Report
Choi, Moonseok
Jang, Hyung-Sup
Son, Taekwon
Kim, Dongsoo
Youn, Young-Jin
Hwang, Gyu-Bin
Choi, Young Pyo
Jeong, Yun Ha
Effect Sizes of Cognitive and Locomotive Behavior Tests in the 5XFAD-J Mouse Model of Alzheimer’s Disease
title Effect Sizes of Cognitive and Locomotive Behavior Tests in the 5XFAD-J Mouse Model of Alzheimer’s Disease
title_full Effect Sizes of Cognitive and Locomotive Behavior Tests in the 5XFAD-J Mouse Model of Alzheimer’s Disease
title_fullStr Effect Sizes of Cognitive and Locomotive Behavior Tests in the 5XFAD-J Mouse Model of Alzheimer’s Disease
title_full_unstemmed Effect Sizes of Cognitive and Locomotive Behavior Tests in the 5XFAD-J Mouse Model of Alzheimer’s Disease
title_short Effect Sizes of Cognitive and Locomotive Behavior Tests in the 5XFAD-J Mouse Model of Alzheimer’s Disease
title_sort effect sizes of cognitive and locomotive behavior tests in the 5xfad-j mouse model of alzheimer’s disease
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10606448/
https://www.ncbi.nlm.nih.gov/pubmed/37894744
http://dx.doi.org/10.3390/ijms242015064
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