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Characterization of the RAD52 Gene in the Budding Yeast Naumovozyma castellii
Several sources of DNA damage compromise the integrity and stability of the genome of every organism. Specifically, DNA double-strand breaks (DSBs) can have lethal consequences for the cell. To repair this type of DNA damage, the cells employ homology-directed repair pathways or non-homologous end j...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10606518/ https://www.ncbi.nlm.nih.gov/pubmed/37895257 http://dx.doi.org/10.3390/genes14101908 |
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author | Itriago, Humberto Marufee Islam, Zubaida Cohn, Marita |
author_facet | Itriago, Humberto Marufee Islam, Zubaida Cohn, Marita |
author_sort | Itriago, Humberto |
collection | PubMed |
description | Several sources of DNA damage compromise the integrity and stability of the genome of every organism. Specifically, DNA double-strand breaks (DSBs) can have lethal consequences for the cell. To repair this type of DNA damage, the cells employ homology-directed repair pathways or non-homologous end joining. Homology-directed repair requires the activity of the RAD52 epistasis group of genes. Rad52 is the main recombination protein in the budding yeast Saccharomyces cerevisiae, and rad52Δ mutants have been characterized to show severe defects in DSB repair and other recombination events. Here, we identified the RAD52 gene in the budding yeast Naumovozyma castellii. Our analysis showed that the primary amino acid sequence of N. castellii Rad52 shared 70% similarity with S. cerevisiae Rad52. To characterize the gene function, we developed rad52Δ mutant strains by targeted gene replacement transformation. We found that N. castellii rad52Δ mutants showed lowered growth capacity, a moderately altered cell morphology and increased sensitivity to genotoxic agents. The decreased viability of the N. castellii rad52Δ mutants in the presence of genotoxic agents indicates that the role of the Rad52 protein in the repair of DNA damage is conserved in this species. |
format | Online Article Text |
id | pubmed-10606518 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-106065182023-10-28 Characterization of the RAD52 Gene in the Budding Yeast Naumovozyma castellii Itriago, Humberto Marufee Islam, Zubaida Cohn, Marita Genes (Basel) Article Several sources of DNA damage compromise the integrity and stability of the genome of every organism. Specifically, DNA double-strand breaks (DSBs) can have lethal consequences for the cell. To repair this type of DNA damage, the cells employ homology-directed repair pathways or non-homologous end joining. Homology-directed repair requires the activity of the RAD52 epistasis group of genes. Rad52 is the main recombination protein in the budding yeast Saccharomyces cerevisiae, and rad52Δ mutants have been characterized to show severe defects in DSB repair and other recombination events. Here, we identified the RAD52 gene in the budding yeast Naumovozyma castellii. Our analysis showed that the primary amino acid sequence of N. castellii Rad52 shared 70% similarity with S. cerevisiae Rad52. To characterize the gene function, we developed rad52Δ mutant strains by targeted gene replacement transformation. We found that N. castellii rad52Δ mutants showed lowered growth capacity, a moderately altered cell morphology and increased sensitivity to genotoxic agents. The decreased viability of the N. castellii rad52Δ mutants in the presence of genotoxic agents indicates that the role of the Rad52 protein in the repair of DNA damage is conserved in this species. MDPI 2023-10-05 /pmc/articles/PMC10606518/ /pubmed/37895257 http://dx.doi.org/10.3390/genes14101908 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Itriago, Humberto Marufee Islam, Zubaida Cohn, Marita Characterization of the RAD52 Gene in the Budding Yeast Naumovozyma castellii |
title | Characterization of the RAD52 Gene in the Budding Yeast Naumovozyma castellii |
title_full | Characterization of the RAD52 Gene in the Budding Yeast Naumovozyma castellii |
title_fullStr | Characterization of the RAD52 Gene in the Budding Yeast Naumovozyma castellii |
title_full_unstemmed | Characterization of the RAD52 Gene in the Budding Yeast Naumovozyma castellii |
title_short | Characterization of the RAD52 Gene in the Budding Yeast Naumovozyma castellii |
title_sort | characterization of the rad52 gene in the budding yeast naumovozyma castellii |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10606518/ https://www.ncbi.nlm.nih.gov/pubmed/37895257 http://dx.doi.org/10.3390/genes14101908 |
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