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The Effects of Pectin–Honey Hydrogel in a Contaminated Chronic Hernia Model in Rats

Incisional hernia is a frequent complication after abdominal surgery. A previous study on rats evaluated the use of a Pectin–Honey Hydrogel (PHH)-coated polypropylene (PP) mesh for the healing of acute hernias. However, there are no studies investigating the use of PHH in association with PP mesh in...

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Autores principales: Cerullo, Anna, Giusto, Gessica, Maniscalco, Lorella, Nebbia, Patrizia, von Degerfeld, Mitzy Mauthe, Serpieri, Matteo, Vercelli, Cristina, Gandini, Marco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10606599/
https://www.ncbi.nlm.nih.gov/pubmed/37888384
http://dx.doi.org/10.3390/gels9100811
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author Cerullo, Anna
Giusto, Gessica
Maniscalco, Lorella
Nebbia, Patrizia
von Degerfeld, Mitzy Mauthe
Serpieri, Matteo
Vercelli, Cristina
Gandini, Marco
author_facet Cerullo, Anna
Giusto, Gessica
Maniscalco, Lorella
Nebbia, Patrizia
von Degerfeld, Mitzy Mauthe
Serpieri, Matteo
Vercelli, Cristina
Gandini, Marco
author_sort Cerullo, Anna
collection PubMed
description Incisional hernia is a frequent complication after abdominal surgery. A previous study on rats evaluated the use of a Pectin–Honey Hydrogel (PHH)-coated polypropylene (PP) mesh for the healing of acute hernias. However, there are no studies investigating the use of PHH in association with PP mesh in chronic contaminated hernia. The aims of this study are to assess the effectiveness of PHH in promoting abdominal hernia repaired with PP mesh and in counteracting infection. Twenty Sprague Dawley male rats were enrolled and a full thickness defect was made in the abdominal wall. The defect was repaired after 28 days using a PP mesh, and a culture medium (Tryptone Soy Broth, Oxoid) was spread onto the mesh to contaminate wounds in both groups. The rats were randomly assigned to a treated or untreated group. In the treated group, a PHH was applied on the mesh before skin closure. At euthanasia—14 days after surgery—macroscopical, microbiological and histopathological evaluations were performed, with a score attributed for signs of inflammation. An immunohistochemical investigation against COX-2 was also performed. Adhesions were more severe (p = 0.0014) and extended (p = 0.0021) in the untreated group. Bacteriological results were not significantly different between groups. Both groups showed moderate to severe values (score > 2) in terms of reparative and inflammatory reactions at histopathological levels. The use of PHH in association with PP mesh could reduce adhesion formation, extension and severity compared to PP mesh alone. No differences in terms of wound healing, contamination and grade of inflammation were reported between groups.
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spelling pubmed-106065992023-10-28 The Effects of Pectin–Honey Hydrogel in a Contaminated Chronic Hernia Model in Rats Cerullo, Anna Giusto, Gessica Maniscalco, Lorella Nebbia, Patrizia von Degerfeld, Mitzy Mauthe Serpieri, Matteo Vercelli, Cristina Gandini, Marco Gels Article Incisional hernia is a frequent complication after abdominal surgery. A previous study on rats evaluated the use of a Pectin–Honey Hydrogel (PHH)-coated polypropylene (PP) mesh for the healing of acute hernias. However, there are no studies investigating the use of PHH in association with PP mesh in chronic contaminated hernia. The aims of this study are to assess the effectiveness of PHH in promoting abdominal hernia repaired with PP mesh and in counteracting infection. Twenty Sprague Dawley male rats were enrolled and a full thickness defect was made in the abdominal wall. The defect was repaired after 28 days using a PP mesh, and a culture medium (Tryptone Soy Broth, Oxoid) was spread onto the mesh to contaminate wounds in both groups. The rats were randomly assigned to a treated or untreated group. In the treated group, a PHH was applied on the mesh before skin closure. At euthanasia—14 days after surgery—macroscopical, microbiological and histopathological evaluations were performed, with a score attributed for signs of inflammation. An immunohistochemical investigation against COX-2 was also performed. Adhesions were more severe (p = 0.0014) and extended (p = 0.0021) in the untreated group. Bacteriological results were not significantly different between groups. Both groups showed moderate to severe values (score > 2) in terms of reparative and inflammatory reactions at histopathological levels. The use of PHH in association with PP mesh could reduce adhesion formation, extension and severity compared to PP mesh alone. No differences in terms of wound healing, contamination and grade of inflammation were reported between groups. MDPI 2023-10-11 /pmc/articles/PMC10606599/ /pubmed/37888384 http://dx.doi.org/10.3390/gels9100811 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cerullo, Anna
Giusto, Gessica
Maniscalco, Lorella
Nebbia, Patrizia
von Degerfeld, Mitzy Mauthe
Serpieri, Matteo
Vercelli, Cristina
Gandini, Marco
The Effects of Pectin–Honey Hydrogel in a Contaminated Chronic Hernia Model in Rats
title The Effects of Pectin–Honey Hydrogel in a Contaminated Chronic Hernia Model in Rats
title_full The Effects of Pectin–Honey Hydrogel in a Contaminated Chronic Hernia Model in Rats
title_fullStr The Effects of Pectin–Honey Hydrogel in a Contaminated Chronic Hernia Model in Rats
title_full_unstemmed The Effects of Pectin–Honey Hydrogel in a Contaminated Chronic Hernia Model in Rats
title_short The Effects of Pectin–Honey Hydrogel in a Contaminated Chronic Hernia Model in Rats
title_sort effects of pectin–honey hydrogel in a contaminated chronic hernia model in rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10606599/
https://www.ncbi.nlm.nih.gov/pubmed/37888384
http://dx.doi.org/10.3390/gels9100811
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