Cargando…
The Effects of Pectin–Honey Hydrogel in a Contaminated Chronic Hernia Model in Rats
Incisional hernia is a frequent complication after abdominal surgery. A previous study on rats evaluated the use of a Pectin–Honey Hydrogel (PHH)-coated polypropylene (PP) mesh for the healing of acute hernias. However, there are no studies investigating the use of PHH in association with PP mesh in...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10606599/ https://www.ncbi.nlm.nih.gov/pubmed/37888384 http://dx.doi.org/10.3390/gels9100811 |
_version_ | 1785127354704592896 |
---|---|
author | Cerullo, Anna Giusto, Gessica Maniscalco, Lorella Nebbia, Patrizia von Degerfeld, Mitzy Mauthe Serpieri, Matteo Vercelli, Cristina Gandini, Marco |
author_facet | Cerullo, Anna Giusto, Gessica Maniscalco, Lorella Nebbia, Patrizia von Degerfeld, Mitzy Mauthe Serpieri, Matteo Vercelli, Cristina Gandini, Marco |
author_sort | Cerullo, Anna |
collection | PubMed |
description | Incisional hernia is a frequent complication after abdominal surgery. A previous study on rats evaluated the use of a Pectin–Honey Hydrogel (PHH)-coated polypropylene (PP) mesh for the healing of acute hernias. However, there are no studies investigating the use of PHH in association with PP mesh in chronic contaminated hernia. The aims of this study are to assess the effectiveness of PHH in promoting abdominal hernia repaired with PP mesh and in counteracting infection. Twenty Sprague Dawley male rats were enrolled and a full thickness defect was made in the abdominal wall. The defect was repaired after 28 days using a PP mesh, and a culture medium (Tryptone Soy Broth, Oxoid) was spread onto the mesh to contaminate wounds in both groups. The rats were randomly assigned to a treated or untreated group. In the treated group, a PHH was applied on the mesh before skin closure. At euthanasia—14 days after surgery—macroscopical, microbiological and histopathological evaluations were performed, with a score attributed for signs of inflammation. An immunohistochemical investigation against COX-2 was also performed. Adhesions were more severe (p = 0.0014) and extended (p = 0.0021) in the untreated group. Bacteriological results were not significantly different between groups. Both groups showed moderate to severe values (score > 2) in terms of reparative and inflammatory reactions at histopathological levels. The use of PHH in association with PP mesh could reduce adhesion formation, extension and severity compared to PP mesh alone. No differences in terms of wound healing, contamination and grade of inflammation were reported between groups. |
format | Online Article Text |
id | pubmed-10606599 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-106065992023-10-28 The Effects of Pectin–Honey Hydrogel in a Contaminated Chronic Hernia Model in Rats Cerullo, Anna Giusto, Gessica Maniscalco, Lorella Nebbia, Patrizia von Degerfeld, Mitzy Mauthe Serpieri, Matteo Vercelli, Cristina Gandini, Marco Gels Article Incisional hernia is a frequent complication after abdominal surgery. A previous study on rats evaluated the use of a Pectin–Honey Hydrogel (PHH)-coated polypropylene (PP) mesh for the healing of acute hernias. However, there are no studies investigating the use of PHH in association with PP mesh in chronic contaminated hernia. The aims of this study are to assess the effectiveness of PHH in promoting abdominal hernia repaired with PP mesh and in counteracting infection. Twenty Sprague Dawley male rats were enrolled and a full thickness defect was made in the abdominal wall. The defect was repaired after 28 days using a PP mesh, and a culture medium (Tryptone Soy Broth, Oxoid) was spread onto the mesh to contaminate wounds in both groups. The rats were randomly assigned to a treated or untreated group. In the treated group, a PHH was applied on the mesh before skin closure. At euthanasia—14 days after surgery—macroscopical, microbiological and histopathological evaluations were performed, with a score attributed for signs of inflammation. An immunohistochemical investigation against COX-2 was also performed. Adhesions were more severe (p = 0.0014) and extended (p = 0.0021) in the untreated group. Bacteriological results were not significantly different between groups. Both groups showed moderate to severe values (score > 2) in terms of reparative and inflammatory reactions at histopathological levels. The use of PHH in association with PP mesh could reduce adhesion formation, extension and severity compared to PP mesh alone. No differences in terms of wound healing, contamination and grade of inflammation were reported between groups. MDPI 2023-10-11 /pmc/articles/PMC10606599/ /pubmed/37888384 http://dx.doi.org/10.3390/gels9100811 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Cerullo, Anna Giusto, Gessica Maniscalco, Lorella Nebbia, Patrizia von Degerfeld, Mitzy Mauthe Serpieri, Matteo Vercelli, Cristina Gandini, Marco The Effects of Pectin–Honey Hydrogel in a Contaminated Chronic Hernia Model in Rats |
title | The Effects of Pectin–Honey Hydrogel in a Contaminated Chronic Hernia Model in Rats |
title_full | The Effects of Pectin–Honey Hydrogel in a Contaminated Chronic Hernia Model in Rats |
title_fullStr | The Effects of Pectin–Honey Hydrogel in a Contaminated Chronic Hernia Model in Rats |
title_full_unstemmed | The Effects of Pectin–Honey Hydrogel in a Contaminated Chronic Hernia Model in Rats |
title_short | The Effects of Pectin–Honey Hydrogel in a Contaminated Chronic Hernia Model in Rats |
title_sort | effects of pectin–honey hydrogel in a contaminated chronic hernia model in rats |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10606599/ https://www.ncbi.nlm.nih.gov/pubmed/37888384 http://dx.doi.org/10.3390/gels9100811 |
work_keys_str_mv | AT cerulloanna theeffectsofpectinhoneyhydrogelinacontaminatedchronicherniamodelinrats AT giustogessica theeffectsofpectinhoneyhydrogelinacontaminatedchronicherniamodelinrats AT maniscalcolorella theeffectsofpectinhoneyhydrogelinacontaminatedchronicherniamodelinrats AT nebbiapatrizia theeffectsofpectinhoneyhydrogelinacontaminatedchronicherniamodelinrats AT vondegerfeldmitzymauthe theeffectsofpectinhoneyhydrogelinacontaminatedchronicherniamodelinrats AT serpierimatteo theeffectsofpectinhoneyhydrogelinacontaminatedchronicherniamodelinrats AT vercellicristina theeffectsofpectinhoneyhydrogelinacontaminatedchronicherniamodelinrats AT gandinimarco theeffectsofpectinhoneyhydrogelinacontaminatedchronicherniamodelinrats AT cerulloanna effectsofpectinhoneyhydrogelinacontaminatedchronicherniamodelinrats AT giustogessica effectsofpectinhoneyhydrogelinacontaminatedchronicherniamodelinrats AT maniscalcolorella effectsofpectinhoneyhydrogelinacontaminatedchronicherniamodelinrats AT nebbiapatrizia effectsofpectinhoneyhydrogelinacontaminatedchronicherniamodelinrats AT vondegerfeldmitzymauthe effectsofpectinhoneyhydrogelinacontaminatedchronicherniamodelinrats AT serpierimatteo effectsofpectinhoneyhydrogelinacontaminatedchronicherniamodelinrats AT vercellicristina effectsofpectinhoneyhydrogelinacontaminatedchronicherniamodelinrats AT gandinimarco effectsofpectinhoneyhydrogelinacontaminatedchronicherniamodelinrats |