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Distinct Pro-Inflammatory Mechanisms Elicited by Short and Long Amosite Asbestos Fibers in Macrophages

While exposure to long amphibolic asbestos fibers (L > 10 µm) results in the development of severe diseases including inflammation, fibrosis, and mesothelioma, the pathogenic activity associated with short fibers (L < 5 µm) is less clear. By exposing murine macrophages to short (SFA) or long (...

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Autores principales: Leinardi, Riccardo, Petriglieri, Jasmine Rita, Pochet, Amandine, Yakoub, Yousof, Lelong, Marie, Lescoat, Alain, Turci, Francesco, Lecureur, Valérie, Huaux, François
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10606797/
https://www.ncbi.nlm.nih.gov/pubmed/37894824
http://dx.doi.org/10.3390/ijms242015145
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author Leinardi, Riccardo
Petriglieri, Jasmine Rita
Pochet, Amandine
Yakoub, Yousof
Lelong, Marie
Lescoat, Alain
Turci, Francesco
Lecureur, Valérie
Huaux, François
author_facet Leinardi, Riccardo
Petriglieri, Jasmine Rita
Pochet, Amandine
Yakoub, Yousof
Lelong, Marie
Lescoat, Alain
Turci, Francesco
Lecureur, Valérie
Huaux, François
author_sort Leinardi, Riccardo
collection PubMed
description While exposure to long amphibolic asbestos fibers (L > 10 µm) results in the development of severe diseases including inflammation, fibrosis, and mesothelioma, the pathogenic activity associated with short fibers (L < 5 µm) is less clear. By exposing murine macrophages to short (SFA) or long (LFA) fibers of amosite asbestos different in size and surface chemistry, we observed that SFA internalization resulted in pyroptotic-related immunogenic cell death (ICD) characterized by the release of the pro-inflammatory damage signal (DAMP) IL-1α after inflammasome activation and gasdermin D (GSDMD)-pore formation. In contrast, macrophage responses to non-internalizable LFA were associated with tumor necrosis factor alpha (TNF-α) release, caspase-3 and -7 activation, and apoptosis. SFA effects exclusively resulted from Toll-like receptor 4 (TLR4), a pattern-recognition receptor (PRR) recognized for its ability to sense particles, while the response to LFA was elicited by a multifactorial ignition system involving the macrophage receptor with collagenous structure (SR-A6 or MARCO), reactive oxygen species (ROS) cascade, and TLR4. Our findings indicate that asbestos fiber size and surface features play major roles in modulating ICD and inflammatory pathways. They also suggest that SFA are biologically reactive in vitro and, therefore, their inflammatory and toxic effects in vivo should not be underestimated.
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spelling pubmed-106067972023-10-28 Distinct Pro-Inflammatory Mechanisms Elicited by Short and Long Amosite Asbestos Fibers in Macrophages Leinardi, Riccardo Petriglieri, Jasmine Rita Pochet, Amandine Yakoub, Yousof Lelong, Marie Lescoat, Alain Turci, Francesco Lecureur, Valérie Huaux, François Int J Mol Sci Article While exposure to long amphibolic asbestos fibers (L > 10 µm) results in the development of severe diseases including inflammation, fibrosis, and mesothelioma, the pathogenic activity associated with short fibers (L < 5 µm) is less clear. By exposing murine macrophages to short (SFA) or long (LFA) fibers of amosite asbestos different in size and surface chemistry, we observed that SFA internalization resulted in pyroptotic-related immunogenic cell death (ICD) characterized by the release of the pro-inflammatory damage signal (DAMP) IL-1α after inflammasome activation and gasdermin D (GSDMD)-pore formation. In contrast, macrophage responses to non-internalizable LFA were associated with tumor necrosis factor alpha (TNF-α) release, caspase-3 and -7 activation, and apoptosis. SFA effects exclusively resulted from Toll-like receptor 4 (TLR4), a pattern-recognition receptor (PRR) recognized for its ability to sense particles, while the response to LFA was elicited by a multifactorial ignition system involving the macrophage receptor with collagenous structure (SR-A6 or MARCO), reactive oxygen species (ROS) cascade, and TLR4. Our findings indicate that asbestos fiber size and surface features play major roles in modulating ICD and inflammatory pathways. They also suggest that SFA are biologically reactive in vitro and, therefore, their inflammatory and toxic effects in vivo should not be underestimated. MDPI 2023-10-13 /pmc/articles/PMC10606797/ /pubmed/37894824 http://dx.doi.org/10.3390/ijms242015145 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Leinardi, Riccardo
Petriglieri, Jasmine Rita
Pochet, Amandine
Yakoub, Yousof
Lelong, Marie
Lescoat, Alain
Turci, Francesco
Lecureur, Valérie
Huaux, François
Distinct Pro-Inflammatory Mechanisms Elicited by Short and Long Amosite Asbestos Fibers in Macrophages
title Distinct Pro-Inflammatory Mechanisms Elicited by Short and Long Amosite Asbestos Fibers in Macrophages
title_full Distinct Pro-Inflammatory Mechanisms Elicited by Short and Long Amosite Asbestos Fibers in Macrophages
title_fullStr Distinct Pro-Inflammatory Mechanisms Elicited by Short and Long Amosite Asbestos Fibers in Macrophages
title_full_unstemmed Distinct Pro-Inflammatory Mechanisms Elicited by Short and Long Amosite Asbestos Fibers in Macrophages
title_short Distinct Pro-Inflammatory Mechanisms Elicited by Short and Long Amosite Asbestos Fibers in Macrophages
title_sort distinct pro-inflammatory mechanisms elicited by short and long amosite asbestos fibers in macrophages
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10606797/
https://www.ncbi.nlm.nih.gov/pubmed/37894824
http://dx.doi.org/10.3390/ijms242015145
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