3′IsomiR Species Composition Affects Reliable Quantification of miRNA/isomiR Variants by Poly(A) RT-qPCR: Impact on Small RNA-Seq Profiling Validation

Small RNA-sequencing (small RNA-seq) has revealed the presence of small RNA-naturally occurring variants such as microRNA (miRNA) isoforms or isomiRs. Due to their small size and the sequence similarity among miRNA isoforms, their validation by RT-qPCR is challenging. We previously identified two mi...

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Detalles Bibliográficos
Autores principales: Ferre, Adriana, Santiago, Lucía, Sánchez-Herrero, José Francisco, López-Rodrigo, Olga, Sánchez-Curbelo, Josvany, Sumoy, Lauro, Bassas, Lluís, Larriba, Sara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10607168/
https://www.ncbi.nlm.nih.gov/pubmed/37895116
http://dx.doi.org/10.3390/ijms242015436

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