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The comprehensive analysis of the prognostic and functional role of N-terminal methyltransferases 1 in pan-cancer
BACKGROUND: NTMT1, a transfer methylase that adds methyl groups to the N-terminus of proteins, has been identified as a critical player in tumor development and progression. However, its precise function in pan-cancer is still unclear. To gain a more comprehensive understanding of its role in cancer...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PeerJ Inc.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10607204/ https://www.ncbi.nlm.nih.gov/pubmed/37901469 http://dx.doi.org/10.7717/peerj.16263 |
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author | Tan, Lifan Li, Wensong Su, Qin |
author_facet | Tan, Lifan Li, Wensong Su, Qin |
author_sort | Tan, Lifan |
collection | PubMed |
description | BACKGROUND: NTMT1, a transfer methylase that adds methyl groups to the N-terminus of proteins, has been identified as a critical player in tumor development and progression. However, its precise function in pan-cancer is still unclear. To gain a more comprehensive understanding of its role in cancer, we performed a thorough bioinformatics analysis. METHODS: To conduct our analysis, we gathered data from multiple sources, including RNA sequencing and clinical data from the TCGA database, protein expression data from the UALCAN and HPA databases, and single-cell expression data from the CancerSEA database. Additionally, we utilized TISIDB to investigate the interaction between the tumor and the immune system. To assess the impact of NTMT1 on the proliferation of SNU1076 cells, we performed a CCK8 assay. We also employed cellular immunofluorescence to detect DNA damage and used flow cytometry to measure tumor cell apoptosis. RESULTS: Our analysis revealed that NTMT1 was significantly overexpressed in various types of tumors and that high levels of NTMT1 were associated with poor survival outcomes. Functional enrichment analysis indicated that NTMT1 may contribute to tumor development and progression by regulating pathways involved in cell proliferation and immune response. In addition, we found that knockdown of NTMT1 expression led to reduced cell proliferation, increased DNA damage, and enhanced apoptosis in HNSCC cells. CONCLUSION: High expression of NTMT1 in tumors is associated with poor prognosis. The underlying regulatory mechanism of NTMT1 in cancer is complex, and it may be involved in both the promotion of tumor development and the inhibition of the tumor immune microenvironment. |
format | Online Article Text |
id | pubmed-10607204 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | PeerJ Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-106072042023-10-28 The comprehensive analysis of the prognostic and functional role of N-terminal methyltransferases 1 in pan-cancer Tan, Lifan Li, Wensong Su, Qin PeerJ Bioinformatics BACKGROUND: NTMT1, a transfer methylase that adds methyl groups to the N-terminus of proteins, has been identified as a critical player in tumor development and progression. However, its precise function in pan-cancer is still unclear. To gain a more comprehensive understanding of its role in cancer, we performed a thorough bioinformatics analysis. METHODS: To conduct our analysis, we gathered data from multiple sources, including RNA sequencing and clinical data from the TCGA database, protein expression data from the UALCAN and HPA databases, and single-cell expression data from the CancerSEA database. Additionally, we utilized TISIDB to investigate the interaction between the tumor and the immune system. To assess the impact of NTMT1 on the proliferation of SNU1076 cells, we performed a CCK8 assay. We also employed cellular immunofluorescence to detect DNA damage and used flow cytometry to measure tumor cell apoptosis. RESULTS: Our analysis revealed that NTMT1 was significantly overexpressed in various types of tumors and that high levels of NTMT1 were associated with poor survival outcomes. Functional enrichment analysis indicated that NTMT1 may contribute to tumor development and progression by regulating pathways involved in cell proliferation and immune response. In addition, we found that knockdown of NTMT1 expression led to reduced cell proliferation, increased DNA damage, and enhanced apoptosis in HNSCC cells. CONCLUSION: High expression of NTMT1 in tumors is associated with poor prognosis. The underlying regulatory mechanism of NTMT1 in cancer is complex, and it may be involved in both the promotion of tumor development and the inhibition of the tumor immune microenvironment. PeerJ Inc. 2023-10-24 /pmc/articles/PMC10607204/ /pubmed/37901469 http://dx.doi.org/10.7717/peerj.16263 Text en © 2023 Tan et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
spellingShingle | Bioinformatics Tan, Lifan Li, Wensong Su, Qin The comprehensive analysis of the prognostic and functional role of N-terminal methyltransferases 1 in pan-cancer |
title | The comprehensive analysis of the prognostic and functional role of N-terminal methyltransferases 1 in pan-cancer |
title_full | The comprehensive analysis of the prognostic and functional role of N-terminal methyltransferases 1 in pan-cancer |
title_fullStr | The comprehensive analysis of the prognostic and functional role of N-terminal methyltransferases 1 in pan-cancer |
title_full_unstemmed | The comprehensive analysis of the prognostic and functional role of N-terminal methyltransferases 1 in pan-cancer |
title_short | The comprehensive analysis of the prognostic and functional role of N-terminal methyltransferases 1 in pan-cancer |
title_sort | comprehensive analysis of the prognostic and functional role of n-terminal methyltransferases 1 in pan-cancer |
topic | Bioinformatics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10607204/ https://www.ncbi.nlm.nih.gov/pubmed/37901469 http://dx.doi.org/10.7717/peerj.16263 |
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