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The comprehensive analysis of the prognostic and functional role of N-terminal methyltransferases 1 in pan-cancer

BACKGROUND: NTMT1, a transfer methylase that adds methyl groups to the N-terminus of proteins, has been identified as a critical player in tumor development and progression. However, its precise function in pan-cancer is still unclear. To gain a more comprehensive understanding of its role in cancer...

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Autores principales: Tan, Lifan, Li, Wensong, Su, Qin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10607204/
https://www.ncbi.nlm.nih.gov/pubmed/37901469
http://dx.doi.org/10.7717/peerj.16263
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author Tan, Lifan
Li, Wensong
Su, Qin
author_facet Tan, Lifan
Li, Wensong
Su, Qin
author_sort Tan, Lifan
collection PubMed
description BACKGROUND: NTMT1, a transfer methylase that adds methyl groups to the N-terminus of proteins, has been identified as a critical player in tumor development and progression. However, its precise function in pan-cancer is still unclear. To gain a more comprehensive understanding of its role in cancer, we performed a thorough bioinformatics analysis. METHODS: To conduct our analysis, we gathered data from multiple sources, including RNA sequencing and clinical data from the TCGA database, protein expression data from the UALCAN and HPA databases, and single-cell expression data from the CancerSEA database. Additionally, we utilized TISIDB to investigate the interaction between the tumor and the immune system. To assess the impact of NTMT1 on the proliferation of SNU1076 cells, we performed a CCK8 assay. We also employed cellular immunofluorescence to detect DNA damage and used flow cytometry to measure tumor cell apoptosis. RESULTS: Our analysis revealed that NTMT1 was significantly overexpressed in various types of tumors and that high levels of NTMT1 were associated with poor survival outcomes. Functional enrichment analysis indicated that NTMT1 may contribute to tumor development and progression by regulating pathways involved in cell proliferation and immune response. In addition, we found that knockdown of NTMT1 expression led to reduced cell proliferation, increased DNA damage, and enhanced apoptosis in HNSCC cells. CONCLUSION: High expression of NTMT1 in tumors is associated with poor prognosis. The underlying regulatory mechanism of NTMT1 in cancer is complex, and it may be involved in both the promotion of tumor development and the inhibition of the tumor immune microenvironment.
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spelling pubmed-106072042023-10-28 The comprehensive analysis of the prognostic and functional role of N-terminal methyltransferases 1 in pan-cancer Tan, Lifan Li, Wensong Su, Qin PeerJ Bioinformatics BACKGROUND: NTMT1, a transfer methylase that adds methyl groups to the N-terminus of proteins, has been identified as a critical player in tumor development and progression. However, its precise function in pan-cancer is still unclear. To gain a more comprehensive understanding of its role in cancer, we performed a thorough bioinformatics analysis. METHODS: To conduct our analysis, we gathered data from multiple sources, including RNA sequencing and clinical data from the TCGA database, protein expression data from the UALCAN and HPA databases, and single-cell expression data from the CancerSEA database. Additionally, we utilized TISIDB to investigate the interaction between the tumor and the immune system. To assess the impact of NTMT1 on the proliferation of SNU1076 cells, we performed a CCK8 assay. We also employed cellular immunofluorescence to detect DNA damage and used flow cytometry to measure tumor cell apoptosis. RESULTS: Our analysis revealed that NTMT1 was significantly overexpressed in various types of tumors and that high levels of NTMT1 were associated with poor survival outcomes. Functional enrichment analysis indicated that NTMT1 may contribute to tumor development and progression by regulating pathways involved in cell proliferation and immune response. In addition, we found that knockdown of NTMT1 expression led to reduced cell proliferation, increased DNA damage, and enhanced apoptosis in HNSCC cells. CONCLUSION: High expression of NTMT1 in tumors is associated with poor prognosis. The underlying regulatory mechanism of NTMT1 in cancer is complex, and it may be involved in both the promotion of tumor development and the inhibition of the tumor immune microenvironment. PeerJ Inc. 2023-10-24 /pmc/articles/PMC10607204/ /pubmed/37901469 http://dx.doi.org/10.7717/peerj.16263 Text en © 2023 Tan et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Bioinformatics
Tan, Lifan
Li, Wensong
Su, Qin
The comprehensive analysis of the prognostic and functional role of N-terminal methyltransferases 1 in pan-cancer
title The comprehensive analysis of the prognostic and functional role of N-terminal methyltransferases 1 in pan-cancer
title_full The comprehensive analysis of the prognostic and functional role of N-terminal methyltransferases 1 in pan-cancer
title_fullStr The comprehensive analysis of the prognostic and functional role of N-terminal methyltransferases 1 in pan-cancer
title_full_unstemmed The comprehensive analysis of the prognostic and functional role of N-terminal methyltransferases 1 in pan-cancer
title_short The comprehensive analysis of the prognostic and functional role of N-terminal methyltransferases 1 in pan-cancer
title_sort comprehensive analysis of the prognostic and functional role of n-terminal methyltransferases 1 in pan-cancer
topic Bioinformatics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10607204/
https://www.ncbi.nlm.nih.gov/pubmed/37901469
http://dx.doi.org/10.7717/peerj.16263
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