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Investigation of the Membrane Localization and Interaction of Selected Flavonoids by NMR and FTIR Spectroscopy
In this report, we discuss the effects of undescribed flavone derivatives, HZ4 and SP9, newly isolated from the aerial parts of Hottonia palustris L. and Scleranthus perennis L. on membranes. Interaction of flavonoids with lipid bilayers is important for medicinal applications. The experiments were...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10607445/ https://www.ncbi.nlm.nih.gov/pubmed/37894955 http://dx.doi.org/10.3390/ijms242015275 |
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author | Kapral-Piotrowska, Justyna Strawa, Jakub W. Jakimiuk, Katarzyna Wiater, Adrian Tomczyk, Michał Gruszecki, Wiesław I. Pawlikowska-Pawlęga, Bożena |
author_facet | Kapral-Piotrowska, Justyna Strawa, Jakub W. Jakimiuk, Katarzyna Wiater, Adrian Tomczyk, Michał Gruszecki, Wiesław I. Pawlikowska-Pawlęga, Bożena |
author_sort | Kapral-Piotrowska, Justyna |
collection | PubMed |
description | In this report, we discuss the effects of undescribed flavone derivatives, HZ4 and SP9, newly isolated from the aerial parts of Hottonia palustris L. and Scleranthus perennis L. on membranes. Interaction of flavonoids with lipid bilayers is important for medicinal applications. The experiments were performed with FTIR and NMR techniques on liposomes prepared from DPPC (dipalmitoylphosphatidylcholine) and EYPC (egg yolk phosphatidylcholine). The data showed that the examined polyphenols incorporate into the polar head group region of DPPC phospholipids at both 25 °C and 45 °C. At the lower temperature, a slight effect in the spectral region of the ester carbonyl group is observed. In contrast, at 45 °C, both compounds bring about the changes in the spectral regions attributed to antisymmetric and symmetric stretching vibrations of CH(2) and CH(3) moieties. Similarly, as in DPPC lipids, the tested compounds interact with the fingerprint region of the polar head groups of the EYPC lipids and cause its reorganization. The outcomes obtained by NMR analyses confirmed the localization of both flavonoids in the polar heads zone. Unraveled effects of HZ4 and SP9 in respect to lipid bilayers can partly determine their biological activities and are crucial for their usability in medicine as disease-preventing phytochemicals. |
format | Online Article Text |
id | pubmed-10607445 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-106074452023-10-28 Investigation of the Membrane Localization and Interaction of Selected Flavonoids by NMR and FTIR Spectroscopy Kapral-Piotrowska, Justyna Strawa, Jakub W. Jakimiuk, Katarzyna Wiater, Adrian Tomczyk, Michał Gruszecki, Wiesław I. Pawlikowska-Pawlęga, Bożena Int J Mol Sci Article In this report, we discuss the effects of undescribed flavone derivatives, HZ4 and SP9, newly isolated from the aerial parts of Hottonia palustris L. and Scleranthus perennis L. on membranes. Interaction of flavonoids with lipid bilayers is important for medicinal applications. The experiments were performed with FTIR and NMR techniques on liposomes prepared from DPPC (dipalmitoylphosphatidylcholine) and EYPC (egg yolk phosphatidylcholine). The data showed that the examined polyphenols incorporate into the polar head group region of DPPC phospholipids at both 25 °C and 45 °C. At the lower temperature, a slight effect in the spectral region of the ester carbonyl group is observed. In contrast, at 45 °C, both compounds bring about the changes in the spectral regions attributed to antisymmetric and symmetric stretching vibrations of CH(2) and CH(3) moieties. Similarly, as in DPPC lipids, the tested compounds interact with the fingerprint region of the polar head groups of the EYPC lipids and cause its reorganization. The outcomes obtained by NMR analyses confirmed the localization of both flavonoids in the polar heads zone. Unraveled effects of HZ4 and SP9 in respect to lipid bilayers can partly determine their biological activities and are crucial for their usability in medicine as disease-preventing phytochemicals. MDPI 2023-10-17 /pmc/articles/PMC10607445/ /pubmed/37894955 http://dx.doi.org/10.3390/ijms242015275 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kapral-Piotrowska, Justyna Strawa, Jakub W. Jakimiuk, Katarzyna Wiater, Adrian Tomczyk, Michał Gruszecki, Wiesław I. Pawlikowska-Pawlęga, Bożena Investigation of the Membrane Localization and Interaction of Selected Flavonoids by NMR and FTIR Spectroscopy |
title | Investigation of the Membrane Localization and Interaction of Selected Flavonoids by NMR and FTIR Spectroscopy |
title_full | Investigation of the Membrane Localization and Interaction of Selected Flavonoids by NMR and FTIR Spectroscopy |
title_fullStr | Investigation of the Membrane Localization and Interaction of Selected Flavonoids by NMR and FTIR Spectroscopy |
title_full_unstemmed | Investigation of the Membrane Localization and Interaction of Selected Flavonoids by NMR and FTIR Spectroscopy |
title_short | Investigation of the Membrane Localization and Interaction of Selected Flavonoids by NMR and FTIR Spectroscopy |
title_sort | investigation of the membrane localization and interaction of selected flavonoids by nmr and ftir spectroscopy |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10607445/ https://www.ncbi.nlm.nih.gov/pubmed/37894955 http://dx.doi.org/10.3390/ijms242015275 |
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