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Closing the Gap in Proteomic Identification of Histoplasma capsulatum: A Case Report and Review of Literature

Histoplasmosis is a globally distributed systemic infection caused by the dimorphic fungus Histoplasma capsulatum (H. capsulatum). This fungus can cause a wide spectrum of clinical manifestations, and the diagnosis of progressive disseminated histoplasmosis is often a challenge for clinicians. Altho...

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Autores principales: Cosio, Terenzio, Gaziano, Roberta, Fontana, Carla, Pistoia, Enrico Salvatore, Petruccelli, Rosalba, Favaro, Marco, Pica, Francesca, Minelli, Silvia, Bossa, Maria Cristina, Altieri, Anna, Ombres, Domenico, Zarabian, Nikkia, D’Agostini, Cartesio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10607645/
https://www.ncbi.nlm.nih.gov/pubmed/37888274
http://dx.doi.org/10.3390/jof9101019
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author Cosio, Terenzio
Gaziano, Roberta
Fontana, Carla
Pistoia, Enrico Salvatore
Petruccelli, Rosalba
Favaro, Marco
Pica, Francesca
Minelli, Silvia
Bossa, Maria Cristina
Altieri, Anna
Ombres, Domenico
Zarabian, Nikkia
D’Agostini, Cartesio
author_facet Cosio, Terenzio
Gaziano, Roberta
Fontana, Carla
Pistoia, Enrico Salvatore
Petruccelli, Rosalba
Favaro, Marco
Pica, Francesca
Minelli, Silvia
Bossa, Maria Cristina
Altieri, Anna
Ombres, Domenico
Zarabian, Nikkia
D’Agostini, Cartesio
author_sort Cosio, Terenzio
collection PubMed
description Histoplasmosis is a globally distributed systemic infection caused by the dimorphic fungus Histoplasma capsulatum (H. capsulatum). This fungus can cause a wide spectrum of clinical manifestations, and the diagnosis of progressive disseminated histoplasmosis is often a challenge for clinicians. Although microscopy and culture remain the gold standard diagnostic tests for Histoplasma identification, matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) has emerged as a method of microbial identification suitable for the confirmation of dimorphic fungi. However, to our knowledge, there are no entries for H. capsulatum spectra in most commercial databases. In this review, we describe the case of disseminated histoplasmosis in a patient living with HIV admitted to our university hospital that we failed to identify by the MALDI-TOF method due to the limited reference spectrum of the instrument database. Furthermore, we highlight the utility of molecular approaches, such as conventional polymerase chain reaction (PCR) and DNA sequencing, as alternative confirmatory tests to MALDI-TOF technology for identifying H. capsulatum from positive cultures. An overview of current evidence and limitations of MALDI-TOF-based characterization of H. capsulatum is also presented.
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spelling pubmed-106076452023-10-28 Closing the Gap in Proteomic Identification of Histoplasma capsulatum: A Case Report and Review of Literature Cosio, Terenzio Gaziano, Roberta Fontana, Carla Pistoia, Enrico Salvatore Petruccelli, Rosalba Favaro, Marco Pica, Francesca Minelli, Silvia Bossa, Maria Cristina Altieri, Anna Ombres, Domenico Zarabian, Nikkia D’Agostini, Cartesio J Fungi (Basel) Review Histoplasmosis is a globally distributed systemic infection caused by the dimorphic fungus Histoplasma capsulatum (H. capsulatum). This fungus can cause a wide spectrum of clinical manifestations, and the diagnosis of progressive disseminated histoplasmosis is often a challenge for clinicians. Although microscopy and culture remain the gold standard diagnostic tests for Histoplasma identification, matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) has emerged as a method of microbial identification suitable for the confirmation of dimorphic fungi. However, to our knowledge, there are no entries for H. capsulatum spectra in most commercial databases. In this review, we describe the case of disseminated histoplasmosis in a patient living with HIV admitted to our university hospital that we failed to identify by the MALDI-TOF method due to the limited reference spectrum of the instrument database. Furthermore, we highlight the utility of molecular approaches, such as conventional polymerase chain reaction (PCR) and DNA sequencing, as alternative confirmatory tests to MALDI-TOF technology for identifying H. capsulatum from positive cultures. An overview of current evidence and limitations of MALDI-TOF-based characterization of H. capsulatum is also presented. MDPI 2023-10-15 /pmc/articles/PMC10607645/ /pubmed/37888274 http://dx.doi.org/10.3390/jof9101019 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Cosio, Terenzio
Gaziano, Roberta
Fontana, Carla
Pistoia, Enrico Salvatore
Petruccelli, Rosalba
Favaro, Marco
Pica, Francesca
Minelli, Silvia
Bossa, Maria Cristina
Altieri, Anna
Ombres, Domenico
Zarabian, Nikkia
D’Agostini, Cartesio
Closing the Gap in Proteomic Identification of Histoplasma capsulatum: A Case Report and Review of Literature
title Closing the Gap in Proteomic Identification of Histoplasma capsulatum: A Case Report and Review of Literature
title_full Closing the Gap in Proteomic Identification of Histoplasma capsulatum: A Case Report and Review of Literature
title_fullStr Closing the Gap in Proteomic Identification of Histoplasma capsulatum: A Case Report and Review of Literature
title_full_unstemmed Closing the Gap in Proteomic Identification of Histoplasma capsulatum: A Case Report and Review of Literature
title_short Closing the Gap in Proteomic Identification of Histoplasma capsulatum: A Case Report and Review of Literature
title_sort closing the gap in proteomic identification of histoplasma capsulatum: a case report and review of literature
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10607645/
https://www.ncbi.nlm.nih.gov/pubmed/37888274
http://dx.doi.org/10.3390/jof9101019
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