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Untargeted Lipidomics Analysis Unravels the Different Metabolites in the Fat Body of Mated Bumblebee (Bombus terrestris) Queens

The fat body has important functions in energy, fertility, and immunity. In female insects, mating stimulates physiological, behavioral, and gene expression changes. However, it remains unclear whether the metabolites in the fat body are affected after the bumblebee (Bombus terrestris) queen mates....

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Autores principales: Guo, Yueqin, Liu, Fugang, Guo, Yulong, Qu, Yingping, Zhang, Zhengyi, Yao, Jun, Xu, Jin, Li, Jilian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10607666/
https://www.ncbi.nlm.nih.gov/pubmed/37895088
http://dx.doi.org/10.3390/ijms242015408
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author Guo, Yueqin
Liu, Fugang
Guo, Yulong
Qu, Yingping
Zhang, Zhengyi
Yao, Jun
Xu, Jin
Li, Jilian
author_facet Guo, Yueqin
Liu, Fugang
Guo, Yulong
Qu, Yingping
Zhang, Zhengyi
Yao, Jun
Xu, Jin
Li, Jilian
author_sort Guo, Yueqin
collection PubMed
description The fat body has important functions in energy, fertility, and immunity. In female insects, mating stimulates physiological, behavioral, and gene expression changes. However, it remains unclear whether the metabolites in the fat body are affected after the bumblebee (Bombus terrestris) queen mates. Here, the ultrastructure and lipid metabolites in fat body of mated queens were compared with those of virgins. The fat body weight of mated bumblebee queens was significantly increased, and the adipocytes were filled with lipid droplets. Using LC-MS/MS-based untargeted lipidomics, 949 and 748 differential metabolites were identified in the fat body of virgin and mated bumblebee queens, respectively, in positive and negative ion modes. Most lipid metabolites were decreased, especially some biomembrane components. In order to explore the relationship between the structures of lipid droplets and metabolite accumulation, transmission electron microscopy and fluorescence microscopy were used to observe the fat body ultrastructure. The size/area of lipid droplets was larger, and the fusion of lipid droplets was increased in the mated queen’s fat body. These enlarged lipid droplets may store more energy and nutrients. The observed differences in lipid metabolites in the fat body of queens contribute to understanding the regulatory network of bumblebees post mating.
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spelling pubmed-106076662023-10-28 Untargeted Lipidomics Analysis Unravels the Different Metabolites in the Fat Body of Mated Bumblebee (Bombus terrestris) Queens Guo, Yueqin Liu, Fugang Guo, Yulong Qu, Yingping Zhang, Zhengyi Yao, Jun Xu, Jin Li, Jilian Int J Mol Sci Article The fat body has important functions in energy, fertility, and immunity. In female insects, mating stimulates physiological, behavioral, and gene expression changes. However, it remains unclear whether the metabolites in the fat body are affected after the bumblebee (Bombus terrestris) queen mates. Here, the ultrastructure and lipid metabolites in fat body of mated queens were compared with those of virgins. The fat body weight of mated bumblebee queens was significantly increased, and the adipocytes were filled with lipid droplets. Using LC-MS/MS-based untargeted lipidomics, 949 and 748 differential metabolites were identified in the fat body of virgin and mated bumblebee queens, respectively, in positive and negative ion modes. Most lipid metabolites were decreased, especially some biomembrane components. In order to explore the relationship between the structures of lipid droplets and metabolite accumulation, transmission electron microscopy and fluorescence microscopy were used to observe the fat body ultrastructure. The size/area of lipid droplets was larger, and the fusion of lipid droplets was increased in the mated queen’s fat body. These enlarged lipid droplets may store more energy and nutrients. The observed differences in lipid metabolites in the fat body of queens contribute to understanding the regulatory network of bumblebees post mating. MDPI 2023-10-21 /pmc/articles/PMC10607666/ /pubmed/37895088 http://dx.doi.org/10.3390/ijms242015408 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Guo, Yueqin
Liu, Fugang
Guo, Yulong
Qu, Yingping
Zhang, Zhengyi
Yao, Jun
Xu, Jin
Li, Jilian
Untargeted Lipidomics Analysis Unravels the Different Metabolites in the Fat Body of Mated Bumblebee (Bombus terrestris) Queens
title Untargeted Lipidomics Analysis Unravels the Different Metabolites in the Fat Body of Mated Bumblebee (Bombus terrestris) Queens
title_full Untargeted Lipidomics Analysis Unravels the Different Metabolites in the Fat Body of Mated Bumblebee (Bombus terrestris) Queens
title_fullStr Untargeted Lipidomics Analysis Unravels the Different Metabolites in the Fat Body of Mated Bumblebee (Bombus terrestris) Queens
title_full_unstemmed Untargeted Lipidomics Analysis Unravels the Different Metabolites in the Fat Body of Mated Bumblebee (Bombus terrestris) Queens
title_short Untargeted Lipidomics Analysis Unravels the Different Metabolites in the Fat Body of Mated Bumblebee (Bombus terrestris) Queens
title_sort untargeted lipidomics analysis unravels the different metabolites in the fat body of mated bumblebee (bombus terrestris) queens
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10607666/
https://www.ncbi.nlm.nih.gov/pubmed/37895088
http://dx.doi.org/10.3390/ijms242015408
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