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Unusual Differences in the Pulmonary Histopathology of Mice after Intranasal Infection with Mycelial Propagules of Histoplasma capsulatum Strains Classified as LAm A2 and NAm 2 Phylogenetic Species

The ascomycete Histoplasma capsulatum is the causative agent of systemic respiratory mycosis histoplasmosis, which sometimes develops acute disseminated or chronic clinical forms, with the latter usually associated with granuloma formation. The present report shows differential histopathological cha...

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Autores principales: Pulido-Camarillo, Evelyn, Sahaza, Jorge H., de Souza Pitangui, Nayla, Mendes-Giannini, Maria José S., Fusco-Almeida, Ana M., Pérez-Torres, Armando, Taylor, Maria Lucia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10607723/
https://www.ncbi.nlm.nih.gov/pubmed/37888230
http://dx.doi.org/10.3390/jof9100974
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author Pulido-Camarillo, Evelyn
Sahaza, Jorge H.
de Souza Pitangui, Nayla
Mendes-Giannini, Maria José S.
Fusco-Almeida, Ana M.
Pérez-Torres, Armando
Taylor, Maria Lucia
author_facet Pulido-Camarillo, Evelyn
Sahaza, Jorge H.
de Souza Pitangui, Nayla
Mendes-Giannini, Maria José S.
Fusco-Almeida, Ana M.
Pérez-Torres, Armando
Taylor, Maria Lucia
author_sort Pulido-Camarillo, Evelyn
collection PubMed
description The ascomycete Histoplasma capsulatum is the causative agent of systemic respiratory mycosis histoplasmosis, which sometimes develops acute disseminated or chronic clinical forms, with the latter usually associated with granuloma formation. The present report shows differential histopathological changes in the pulmonary inflammatory response of mice infected intranasally with the mycelial morphotype of H. capsulatum strains with distinct genotypes, EH-46 and G-217B, classified as LAm A2 and NAm 2 phylogenetic species, respectively. Infected male BALB/c mice were sacrificed at different postinfection times, and their serial lung sections were stained with periodic acid–Schiff and analyzed via microscopy. In mice infected with the LAm A2 strain, the results showed progressive changes in the inflammatory infiltrate of the lung parenchyma during the first hours and days postinfection as well as granulomas with macrophages containing intracellular yeast cells, which prevailed at 14 and 21 days postinfection. Bronchiolar-associated lymphoid tissue was induced in mice infected with both strains, primarily in mice infected with the NAm 2 strain. Several lung sections from mice infected with the LAm A2 strain showed PAS-positive yeast cells aggregated in a perinuclear crown-like arrangement in macrophages from 3 h to 21 days postinfection. These findings highlight differences in the host pulmonary inflammatory response associated with distinct H. capsulatum species.
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spelling pubmed-106077232023-10-28 Unusual Differences in the Pulmonary Histopathology of Mice after Intranasal Infection with Mycelial Propagules of Histoplasma capsulatum Strains Classified as LAm A2 and NAm 2 Phylogenetic Species Pulido-Camarillo, Evelyn Sahaza, Jorge H. de Souza Pitangui, Nayla Mendes-Giannini, Maria José S. Fusco-Almeida, Ana M. Pérez-Torres, Armando Taylor, Maria Lucia J Fungi (Basel) Article The ascomycete Histoplasma capsulatum is the causative agent of systemic respiratory mycosis histoplasmosis, which sometimes develops acute disseminated or chronic clinical forms, with the latter usually associated with granuloma formation. The present report shows differential histopathological changes in the pulmonary inflammatory response of mice infected intranasally with the mycelial morphotype of H. capsulatum strains with distinct genotypes, EH-46 and G-217B, classified as LAm A2 and NAm 2 phylogenetic species, respectively. Infected male BALB/c mice were sacrificed at different postinfection times, and their serial lung sections were stained with periodic acid–Schiff and analyzed via microscopy. In mice infected with the LAm A2 strain, the results showed progressive changes in the inflammatory infiltrate of the lung parenchyma during the first hours and days postinfection as well as granulomas with macrophages containing intracellular yeast cells, which prevailed at 14 and 21 days postinfection. Bronchiolar-associated lymphoid tissue was induced in mice infected with both strains, primarily in mice infected with the NAm 2 strain. Several lung sections from mice infected with the LAm A2 strain showed PAS-positive yeast cells aggregated in a perinuclear crown-like arrangement in macrophages from 3 h to 21 days postinfection. These findings highlight differences in the host pulmonary inflammatory response associated with distinct H. capsulatum species. MDPI 2023-09-27 /pmc/articles/PMC10607723/ /pubmed/37888230 http://dx.doi.org/10.3390/jof9100974 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pulido-Camarillo, Evelyn
Sahaza, Jorge H.
de Souza Pitangui, Nayla
Mendes-Giannini, Maria José S.
Fusco-Almeida, Ana M.
Pérez-Torres, Armando
Taylor, Maria Lucia
Unusual Differences in the Pulmonary Histopathology of Mice after Intranasal Infection with Mycelial Propagules of Histoplasma capsulatum Strains Classified as LAm A2 and NAm 2 Phylogenetic Species
title Unusual Differences in the Pulmonary Histopathology of Mice after Intranasal Infection with Mycelial Propagules of Histoplasma capsulatum Strains Classified as LAm A2 and NAm 2 Phylogenetic Species
title_full Unusual Differences in the Pulmonary Histopathology of Mice after Intranasal Infection with Mycelial Propagules of Histoplasma capsulatum Strains Classified as LAm A2 and NAm 2 Phylogenetic Species
title_fullStr Unusual Differences in the Pulmonary Histopathology of Mice after Intranasal Infection with Mycelial Propagules of Histoplasma capsulatum Strains Classified as LAm A2 and NAm 2 Phylogenetic Species
title_full_unstemmed Unusual Differences in the Pulmonary Histopathology of Mice after Intranasal Infection with Mycelial Propagules of Histoplasma capsulatum Strains Classified as LAm A2 and NAm 2 Phylogenetic Species
title_short Unusual Differences in the Pulmonary Histopathology of Mice after Intranasal Infection with Mycelial Propagules of Histoplasma capsulatum Strains Classified as LAm A2 and NAm 2 Phylogenetic Species
title_sort unusual differences in the pulmonary histopathology of mice after intranasal infection with mycelial propagules of histoplasma capsulatum strains classified as lam a2 and nam 2 phylogenetic species
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10607723/
https://www.ncbi.nlm.nih.gov/pubmed/37888230
http://dx.doi.org/10.3390/jof9100974
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