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Hepatoprotective and Antioxidant Effects of Nanopiperine against Cypermethrin via Mitigation of Oxidative Stress, Inflammations and Gene Expression Using qRT-PCR

Cypermethrin (Cyp) is a pyrethroid that has been associated with the toxicity of various organs. The aim of our study was to evaluate the hepatoprotective and antioxidant activities of nano-piperine (NP) against Cyp toxicity. Cyp (50 mg/kg) was administered orally in all animals of groups III–VI for...

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Autores principales: Hussain, Sohail, Jali, Abdulmajeed M., Alshahrani, Saeed, Khairat, Khairat H. M., Siddiqui, Rahimullah, Alam, Mohammad Intakhab, Ali, Raisuddin, Mohammed, Manal, Khan, Andleeb, Al Shahi, Hamad, Hanbashi, Ali, Qadri, Marwa, Ashafaq, Mohammad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10607774/
https://www.ncbi.nlm.nih.gov/pubmed/37895045
http://dx.doi.org/10.3390/ijms242015361
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author Hussain, Sohail
Jali, Abdulmajeed M.
Alshahrani, Saeed
Khairat, Khairat H. M.
Siddiqui, Rahimullah
Alam, Mohammad Intakhab
Ali, Raisuddin
Mohammed, Manal
Khan, Andleeb
Al Shahi, Hamad
Hanbashi, Ali
Qadri, Marwa
Ashafaq, Mohammad
author_facet Hussain, Sohail
Jali, Abdulmajeed M.
Alshahrani, Saeed
Khairat, Khairat H. M.
Siddiqui, Rahimullah
Alam, Mohammad Intakhab
Ali, Raisuddin
Mohammed, Manal
Khan, Andleeb
Al Shahi, Hamad
Hanbashi, Ali
Qadri, Marwa
Ashafaq, Mohammad
author_sort Hussain, Sohail
collection PubMed
description Cypermethrin (Cyp) is a pyrethroid that has been associated with the toxicity of various organs. The aim of our study was to evaluate the hepatoprotective and antioxidant activities of nano-piperine (NP) against Cyp toxicity. Cyp (50 mg/kg) was administered orally in all animals of groups III–VI for 15 days. Groups IV–VI each received three doses of NP (125, 250, and 500 µg/kg/day) for 10 days after receiving the Cyp dosage, which was given after 1 h. A rise in serum biomarkers (ALT, AST, ALP, total protein, and albumin), which are indicators of toxicity alongside anomalous oxidative stress indices (lipid peroxidation (LPO), glutathione (GSH), superoxide dismutase (SOD) and catalase), was detected. After Cyp treatment, we observed upregulated cytokines, caspase expression, and histological analysis that the showed distortion of cell shape. However, the administration of NP dramatically reversed all of the Cyp-induced alterations, inducing reductions in serum marker levels, stress level, the production of cytokines, and caspase expression. Additionally, all of the histopathological alterations were minimized to values that were comparable to normal levels. The present findings suggested that NP exhibits potent antioxidant and anti-inflammatory activities that can protect rats’ livers against Cyp-induced liver damage through hepatoprotective activities.
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spelling pubmed-106077742023-10-28 Hepatoprotective and Antioxidant Effects of Nanopiperine against Cypermethrin via Mitigation of Oxidative Stress, Inflammations and Gene Expression Using qRT-PCR Hussain, Sohail Jali, Abdulmajeed M. Alshahrani, Saeed Khairat, Khairat H. M. Siddiqui, Rahimullah Alam, Mohammad Intakhab Ali, Raisuddin Mohammed, Manal Khan, Andleeb Al Shahi, Hamad Hanbashi, Ali Qadri, Marwa Ashafaq, Mohammad Int J Mol Sci Article Cypermethrin (Cyp) is a pyrethroid that has been associated with the toxicity of various organs. The aim of our study was to evaluate the hepatoprotective and antioxidant activities of nano-piperine (NP) against Cyp toxicity. Cyp (50 mg/kg) was administered orally in all animals of groups III–VI for 15 days. Groups IV–VI each received three doses of NP (125, 250, and 500 µg/kg/day) for 10 days after receiving the Cyp dosage, which was given after 1 h. A rise in serum biomarkers (ALT, AST, ALP, total protein, and albumin), which are indicators of toxicity alongside anomalous oxidative stress indices (lipid peroxidation (LPO), glutathione (GSH), superoxide dismutase (SOD) and catalase), was detected. After Cyp treatment, we observed upregulated cytokines, caspase expression, and histological analysis that the showed distortion of cell shape. However, the administration of NP dramatically reversed all of the Cyp-induced alterations, inducing reductions in serum marker levels, stress level, the production of cytokines, and caspase expression. Additionally, all of the histopathological alterations were minimized to values that were comparable to normal levels. The present findings suggested that NP exhibits potent antioxidant and anti-inflammatory activities that can protect rats’ livers against Cyp-induced liver damage through hepatoprotective activities. MDPI 2023-10-19 /pmc/articles/PMC10607774/ /pubmed/37895045 http://dx.doi.org/10.3390/ijms242015361 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hussain, Sohail
Jali, Abdulmajeed M.
Alshahrani, Saeed
Khairat, Khairat H. M.
Siddiqui, Rahimullah
Alam, Mohammad Intakhab
Ali, Raisuddin
Mohammed, Manal
Khan, Andleeb
Al Shahi, Hamad
Hanbashi, Ali
Qadri, Marwa
Ashafaq, Mohammad
Hepatoprotective and Antioxidant Effects of Nanopiperine against Cypermethrin via Mitigation of Oxidative Stress, Inflammations and Gene Expression Using qRT-PCR
title Hepatoprotective and Antioxidant Effects of Nanopiperine against Cypermethrin via Mitigation of Oxidative Stress, Inflammations and Gene Expression Using qRT-PCR
title_full Hepatoprotective and Antioxidant Effects of Nanopiperine against Cypermethrin via Mitigation of Oxidative Stress, Inflammations and Gene Expression Using qRT-PCR
title_fullStr Hepatoprotective and Antioxidant Effects of Nanopiperine against Cypermethrin via Mitigation of Oxidative Stress, Inflammations and Gene Expression Using qRT-PCR
title_full_unstemmed Hepatoprotective and Antioxidant Effects of Nanopiperine against Cypermethrin via Mitigation of Oxidative Stress, Inflammations and Gene Expression Using qRT-PCR
title_short Hepatoprotective and Antioxidant Effects of Nanopiperine against Cypermethrin via Mitigation of Oxidative Stress, Inflammations and Gene Expression Using qRT-PCR
title_sort hepatoprotective and antioxidant effects of nanopiperine against cypermethrin via mitigation of oxidative stress, inflammations and gene expression using qrt-pcr
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10607774/
https://www.ncbi.nlm.nih.gov/pubmed/37895045
http://dx.doi.org/10.3390/ijms242015361
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