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Smoking and Diabetes Attenuate Number of CD34(+) Haematopoietic Stem Cells in Peripheral Blood of Patients with Advanced Peripheral Artery Disease
Peripheral artery disease (PAD) is a globally prevalent problem with limited treatment options, leaving up to a fifth of patients remediless. The emergence of new studies on cell therapy in recent years offers a new promising option for their treatment. Our aim was to explore how the number of CD34(...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10607776/ https://www.ncbi.nlm.nih.gov/pubmed/37895025 http://dx.doi.org/10.3390/ijms242015346 |
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author | Sernek, Barbara Kamnikar, Rok Sebestjen, Miran Boc, Anja Boc, Vinko |
author_facet | Sernek, Barbara Kamnikar, Rok Sebestjen, Miran Boc, Anja Boc, Vinko |
author_sort | Sernek, Barbara |
collection | PubMed |
description | Peripheral artery disease (PAD) is a globally prevalent problem with limited treatment options, leaving up to a fifth of patients remediless. The emergence of new studies on cell therapy in recent years offers a new promising option for their treatment. Our aim was to explore how the number of CD34(+) hematopoietic cells in the peripheral blood of PAD patients is associated with patients’ functional as well as atherogenic factors. We selected 30 patients with advanced PAD, recorded their performance in a walking test in standard conditions and sampled their blood for further analysis with an emphasis on CD34(+) cell selection and counting. No correlation of the CD34(+) cell number was confirmed with any of the observed laboratory parameters. There was an association between the claudication distance and the number of CD34(+) cells (r = −0.403, p = 0.046). The number of CD34(+) cells differed between patients with and without type II diabetes (p = 0.071) and between active smokers, past smokers, and non-smokers (p = 0.035; p = 0.068, p = 0.051, respectively), with both smoking and presence of diabetes type II having a negative effect on the number of CD34(+) cells. Our study demonstrated a dependence of the CD34(+) cell number on the patient’s characteristics. |
format | Online Article Text |
id | pubmed-10607776 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-106077762023-10-28 Smoking and Diabetes Attenuate Number of CD34(+) Haematopoietic Stem Cells in Peripheral Blood of Patients with Advanced Peripheral Artery Disease Sernek, Barbara Kamnikar, Rok Sebestjen, Miran Boc, Anja Boc, Vinko Int J Mol Sci Article Peripheral artery disease (PAD) is a globally prevalent problem with limited treatment options, leaving up to a fifth of patients remediless. The emergence of new studies on cell therapy in recent years offers a new promising option for their treatment. Our aim was to explore how the number of CD34(+) hematopoietic cells in the peripheral blood of PAD patients is associated with patients’ functional as well as atherogenic factors. We selected 30 patients with advanced PAD, recorded their performance in a walking test in standard conditions and sampled their blood for further analysis with an emphasis on CD34(+) cell selection and counting. No correlation of the CD34(+) cell number was confirmed with any of the observed laboratory parameters. There was an association between the claudication distance and the number of CD34(+) cells (r = −0.403, p = 0.046). The number of CD34(+) cells differed between patients with and without type II diabetes (p = 0.071) and between active smokers, past smokers, and non-smokers (p = 0.035; p = 0.068, p = 0.051, respectively), with both smoking and presence of diabetes type II having a negative effect on the number of CD34(+) cells. Our study demonstrated a dependence of the CD34(+) cell number on the patient’s characteristics. MDPI 2023-10-19 /pmc/articles/PMC10607776/ /pubmed/37895025 http://dx.doi.org/10.3390/ijms242015346 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Sernek, Barbara Kamnikar, Rok Sebestjen, Miran Boc, Anja Boc, Vinko Smoking and Diabetes Attenuate Number of CD34(+) Haematopoietic Stem Cells in Peripheral Blood of Patients with Advanced Peripheral Artery Disease |
title | Smoking and Diabetes Attenuate Number of CD34(+) Haematopoietic Stem Cells in Peripheral Blood of Patients with Advanced Peripheral Artery Disease |
title_full | Smoking and Diabetes Attenuate Number of CD34(+) Haematopoietic Stem Cells in Peripheral Blood of Patients with Advanced Peripheral Artery Disease |
title_fullStr | Smoking and Diabetes Attenuate Number of CD34(+) Haematopoietic Stem Cells in Peripheral Blood of Patients with Advanced Peripheral Artery Disease |
title_full_unstemmed | Smoking and Diabetes Attenuate Number of CD34(+) Haematopoietic Stem Cells in Peripheral Blood of Patients with Advanced Peripheral Artery Disease |
title_short | Smoking and Diabetes Attenuate Number of CD34(+) Haematopoietic Stem Cells in Peripheral Blood of Patients with Advanced Peripheral Artery Disease |
title_sort | smoking and diabetes attenuate number of cd34(+) haematopoietic stem cells in peripheral blood of patients with advanced peripheral artery disease |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10607776/ https://www.ncbi.nlm.nih.gov/pubmed/37895025 http://dx.doi.org/10.3390/ijms242015346 |
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