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An Overview of Systematic Reviews of Polymerase Chain Reaction (PCR) for the Diagnosis of Invasive Aspergillosis in Immunocompromised People: A Report of the Fungal PCR Initiative (FPCRI)—An ISHAM Working Group

This overview of reviews (i.e., an umbrella review) is designed to reappraise the validity of systematic reviews (SRs) and meta-analyses related to the performance of Aspergillus PCR tests for the diagnosis of invasive aspergillosis in immunocompromised patients. The methodological quality of the SR...

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Autores principales: Cruciani, Mario, White, P. Lewis, Barnes, Rosemary A., Loeffler, Juergen, Donnelly, J. Peter, Rogers, Thomas R., Heinz, Werner J., Warris, Adilia, Morton, Charles Oliver, Lengerova, Martina, Klingspor, Lena, Sendid, Boualem, Lockhart, Deborah E. A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10607919/
https://www.ncbi.nlm.nih.gov/pubmed/37888223
http://dx.doi.org/10.3390/jof9100967
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author Cruciani, Mario
White, P. Lewis
Barnes, Rosemary A.
Loeffler, Juergen
Donnelly, J. Peter
Rogers, Thomas R.
Heinz, Werner J.
Warris, Adilia
Morton, Charles Oliver
Lengerova, Martina
Klingspor, Lena
Sendid, Boualem
Lockhart, Deborah E. A.
author_facet Cruciani, Mario
White, P. Lewis
Barnes, Rosemary A.
Loeffler, Juergen
Donnelly, J. Peter
Rogers, Thomas R.
Heinz, Werner J.
Warris, Adilia
Morton, Charles Oliver
Lengerova, Martina
Klingspor, Lena
Sendid, Boualem
Lockhart, Deborah E. A.
author_sort Cruciani, Mario
collection PubMed
description This overview of reviews (i.e., an umbrella review) is designed to reappraise the validity of systematic reviews (SRs) and meta-analyses related to the performance of Aspergillus PCR tests for the diagnosis of invasive aspergillosis in immunocompromised patients. The methodological quality of the SRs was assessed using the AMSTAR-2 checklist; the quality of the evidence (QOE) within each SR was appraised following the GRADE approach. Eight out of 12 SRs were evaluated for qualitative and quantitative assessment. Five SRs evaluated Aspergillus PCR on bronchoalveolar lavage fluid (BAL) and three on blood specimens. The eight SRs included 167 overlapping reports (59 evaluating PCR in blood specimens, and 108 in BAL), based on 107 individual primary studies (98 trials with a cohort design, and 19 with a case−control design). In BAL specimens, the mean sensitivity and specificity ranged from 0.57 to 0.91, and from 0.92 to 0.97, respectively (QOE: very low to low). In blood specimens (whole blood or serum), the mean sensitivity ranged from 0.57 to 0.84, and the mean specificity from 0.58 to 0.95 (QOE: low to moderate). Across studies, only a low proportion of AMSTAR-2 critical domains were unmet (1.8%), demonstrating a high quality of methodological assessment. Conclusions. Based on the overall methodological assessment of the reviews included, on average we can have high confidence in the quality of results generated by the SRs.
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spelling pubmed-106079192023-10-28 An Overview of Systematic Reviews of Polymerase Chain Reaction (PCR) for the Diagnosis of Invasive Aspergillosis in Immunocompromised People: A Report of the Fungal PCR Initiative (FPCRI)—An ISHAM Working Group Cruciani, Mario White, P. Lewis Barnes, Rosemary A. Loeffler, Juergen Donnelly, J. Peter Rogers, Thomas R. Heinz, Werner J. Warris, Adilia Morton, Charles Oliver Lengerova, Martina Klingspor, Lena Sendid, Boualem Lockhart, Deborah E. A. J Fungi (Basel) Systematic Review This overview of reviews (i.e., an umbrella review) is designed to reappraise the validity of systematic reviews (SRs) and meta-analyses related to the performance of Aspergillus PCR tests for the diagnosis of invasive aspergillosis in immunocompromised patients. The methodological quality of the SRs was assessed using the AMSTAR-2 checklist; the quality of the evidence (QOE) within each SR was appraised following the GRADE approach. Eight out of 12 SRs were evaluated for qualitative and quantitative assessment. Five SRs evaluated Aspergillus PCR on bronchoalveolar lavage fluid (BAL) and three on blood specimens. The eight SRs included 167 overlapping reports (59 evaluating PCR in blood specimens, and 108 in BAL), based on 107 individual primary studies (98 trials with a cohort design, and 19 with a case−control design). In BAL specimens, the mean sensitivity and specificity ranged from 0.57 to 0.91, and from 0.92 to 0.97, respectively (QOE: very low to low). In blood specimens (whole blood or serum), the mean sensitivity ranged from 0.57 to 0.84, and the mean specificity from 0.58 to 0.95 (QOE: low to moderate). Across studies, only a low proportion of AMSTAR-2 critical domains were unmet (1.8%), demonstrating a high quality of methodological assessment. Conclusions. Based on the overall methodological assessment of the reviews included, on average we can have high confidence in the quality of results generated by the SRs. MDPI 2023-09-26 /pmc/articles/PMC10607919/ /pubmed/37888223 http://dx.doi.org/10.3390/jof9100967 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Systematic Review
Cruciani, Mario
White, P. Lewis
Barnes, Rosemary A.
Loeffler, Juergen
Donnelly, J. Peter
Rogers, Thomas R.
Heinz, Werner J.
Warris, Adilia
Morton, Charles Oliver
Lengerova, Martina
Klingspor, Lena
Sendid, Boualem
Lockhart, Deborah E. A.
An Overview of Systematic Reviews of Polymerase Chain Reaction (PCR) for the Diagnosis of Invasive Aspergillosis in Immunocompromised People: A Report of the Fungal PCR Initiative (FPCRI)—An ISHAM Working Group
title An Overview of Systematic Reviews of Polymerase Chain Reaction (PCR) for the Diagnosis of Invasive Aspergillosis in Immunocompromised People: A Report of the Fungal PCR Initiative (FPCRI)—An ISHAM Working Group
title_full An Overview of Systematic Reviews of Polymerase Chain Reaction (PCR) for the Diagnosis of Invasive Aspergillosis in Immunocompromised People: A Report of the Fungal PCR Initiative (FPCRI)—An ISHAM Working Group
title_fullStr An Overview of Systematic Reviews of Polymerase Chain Reaction (PCR) for the Diagnosis of Invasive Aspergillosis in Immunocompromised People: A Report of the Fungal PCR Initiative (FPCRI)—An ISHAM Working Group
title_full_unstemmed An Overview of Systematic Reviews of Polymerase Chain Reaction (PCR) for the Diagnosis of Invasive Aspergillosis in Immunocompromised People: A Report of the Fungal PCR Initiative (FPCRI)—An ISHAM Working Group
title_short An Overview of Systematic Reviews of Polymerase Chain Reaction (PCR) for the Diagnosis of Invasive Aspergillosis in Immunocompromised People: A Report of the Fungal PCR Initiative (FPCRI)—An ISHAM Working Group
title_sort overview of systematic reviews of polymerase chain reaction (pcr) for the diagnosis of invasive aspergillosis in immunocompromised people: a report of the fungal pcr initiative (fpcri)—an isham working group
topic Systematic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10607919/
https://www.ncbi.nlm.nih.gov/pubmed/37888223
http://dx.doi.org/10.3390/jof9100967
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