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Protein Disulfide Isomerase FgEps1 Is a Secreted Virulence Factor in Fusarium graminearum

Protein disulfide isomerase (PDI) is a member of the thioredoxin (Trx) superfamily with important functions in cellular stability, ion uptake, and cellular differentiation. While PDI has been extensively studied in humans and animals, its role in fungi remains relatively unknown. In this study, the...

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Autores principales: Liu, Kouhan, Wang, Xintong, Li, Ying, Shi, Yifeng, Ren, Yanyan, Wang, Aolin, Zhao, Bingjie, Cheng, Peng, Wang, Baotong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10607971/
https://www.ncbi.nlm.nih.gov/pubmed/37888265
http://dx.doi.org/10.3390/jof9101009
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author Liu, Kouhan
Wang, Xintong
Li, Ying
Shi, Yifeng
Ren, Yanyan
Wang, Aolin
Zhao, Bingjie
Cheng, Peng
Wang, Baotong
author_facet Liu, Kouhan
Wang, Xintong
Li, Ying
Shi, Yifeng
Ren, Yanyan
Wang, Aolin
Zhao, Bingjie
Cheng, Peng
Wang, Baotong
author_sort Liu, Kouhan
collection PubMed
description Protein disulfide isomerase (PDI) is a member of the thioredoxin (Trx) superfamily with important functions in cellular stability, ion uptake, and cellular differentiation. While PDI has been extensively studied in humans and animals, its role in fungi remains relatively unknown. In this study, the biological functions of FgEps1, a disulfide bond isomerase in the fungal pathogen Fusarium graminearum, were investigated. It was found that FgEps1 mutation affected nutritional growth, asexual and sexual reproduction, and stress tolerance. Additionally, its deletion resulted in reduced pathogenicity and impaired DON toxin biosynthesis. The involvement of FgEps1 in host infection was also confirmed, as its expression was detected during the infection period. Further investigation using a yeast signal peptide secretion system and transient expression in Nicotiana benthamiana showed that FgEps1 suppressed the immune response of plants and promoted infection. These findings suggest that virulence factor FgEps1 plays a crucial role in growth, development, virulence, secondary metabolism, and host infection in F. graminearum.
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spelling pubmed-106079712023-10-28 Protein Disulfide Isomerase FgEps1 Is a Secreted Virulence Factor in Fusarium graminearum Liu, Kouhan Wang, Xintong Li, Ying Shi, Yifeng Ren, Yanyan Wang, Aolin Zhao, Bingjie Cheng, Peng Wang, Baotong J Fungi (Basel) Article Protein disulfide isomerase (PDI) is a member of the thioredoxin (Trx) superfamily with important functions in cellular stability, ion uptake, and cellular differentiation. While PDI has been extensively studied in humans and animals, its role in fungi remains relatively unknown. In this study, the biological functions of FgEps1, a disulfide bond isomerase in the fungal pathogen Fusarium graminearum, were investigated. It was found that FgEps1 mutation affected nutritional growth, asexual and sexual reproduction, and stress tolerance. Additionally, its deletion resulted in reduced pathogenicity and impaired DON toxin biosynthesis. The involvement of FgEps1 in host infection was also confirmed, as its expression was detected during the infection period. Further investigation using a yeast signal peptide secretion system and transient expression in Nicotiana benthamiana showed that FgEps1 suppressed the immune response of plants and promoted infection. These findings suggest that virulence factor FgEps1 plays a crucial role in growth, development, virulence, secondary metabolism, and host infection in F. graminearum. MDPI 2023-10-12 /pmc/articles/PMC10607971/ /pubmed/37888265 http://dx.doi.org/10.3390/jof9101009 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Kouhan
Wang, Xintong
Li, Ying
Shi, Yifeng
Ren, Yanyan
Wang, Aolin
Zhao, Bingjie
Cheng, Peng
Wang, Baotong
Protein Disulfide Isomerase FgEps1 Is a Secreted Virulence Factor in Fusarium graminearum
title Protein Disulfide Isomerase FgEps1 Is a Secreted Virulence Factor in Fusarium graminearum
title_full Protein Disulfide Isomerase FgEps1 Is a Secreted Virulence Factor in Fusarium graminearum
title_fullStr Protein Disulfide Isomerase FgEps1 Is a Secreted Virulence Factor in Fusarium graminearum
title_full_unstemmed Protein Disulfide Isomerase FgEps1 Is a Secreted Virulence Factor in Fusarium graminearum
title_short Protein Disulfide Isomerase FgEps1 Is a Secreted Virulence Factor in Fusarium graminearum
title_sort protein disulfide isomerase fgeps1 is a secreted virulence factor in fusarium graminearum
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10607971/
https://www.ncbi.nlm.nih.gov/pubmed/37888265
http://dx.doi.org/10.3390/jof9101009
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