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Identification of Glycoxidative Lesion in Isolated Low-Density Lipoproteins from Diabetes Mellitus Subjects
Methylglyoxal (MG) is a precursor for advanced glycation end-products (AGEs), which have a significant role in diabetes. The present study is designed to probe the immunological response of native and glycated low-density lipoprotein (LDL) in experimental animals. The second part of this study is to...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10608319/ https://www.ncbi.nlm.nih.gov/pubmed/37895368 http://dx.doi.org/10.3390/life13101986 |
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author | Alyahyawi, Amjad R. Khan, Mohd Yasir Alouffi, Sultan Maarfi, Farah Akasha, Rihab Khan, Saif Rafi, Zeeshan Alharazi, Talal Shahab, Uzma Ahmad, Saheem |
author_facet | Alyahyawi, Amjad R. Khan, Mohd Yasir Alouffi, Sultan Maarfi, Farah Akasha, Rihab Khan, Saif Rafi, Zeeshan Alharazi, Talal Shahab, Uzma Ahmad, Saheem |
author_sort | Alyahyawi, Amjad R. |
collection | PubMed |
description | Methylglyoxal (MG) is a precursor for advanced glycation end-products (AGEs), which have a significant role in diabetes. The present study is designed to probe the immunological response of native and glycated low-density lipoprotein (LDL) in experimental animals. The second part of this study is to probe glycoxidative lesion detection in low-density lipoproteins (LDL) in diabetes subjects with varying disease duration. The neo-epitopes attributed to glycation-induced glycoxidative lesion of LDL in DM patients’ plasma were, analyzed by binding of native and MG-modified LDL immunized animal sera antibodies using an immunochemical assay. The plasma purified human LDL glycation with MG, which instigated modification in LDL. Further, the NewZealand-White rabbits were infused with unmodified natural LDL (N-LDL) and MG-glycatedLDL to probe its immunogenicity. The glycoxidative lesion detection in LDL of DM with disease duration (D.D.) of 5–15 years and D.D. > 15 years was found to be significantly higher as compared to normal healthy subjects (NHS) LDL. The findings support the notion that prolonged duration of diabetes can cause structural alteration in LDL protein molecules, rendering them highly immunogenic in nature. The presence of LDL lesions specific to MG-associated glycoxidation would further help in assessing the progression of diabetes mellitus. |
format | Online Article Text |
id | pubmed-10608319 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-106083192023-10-28 Identification of Glycoxidative Lesion in Isolated Low-Density Lipoproteins from Diabetes Mellitus Subjects Alyahyawi, Amjad R. Khan, Mohd Yasir Alouffi, Sultan Maarfi, Farah Akasha, Rihab Khan, Saif Rafi, Zeeshan Alharazi, Talal Shahab, Uzma Ahmad, Saheem Life (Basel) Article Methylglyoxal (MG) is a precursor for advanced glycation end-products (AGEs), which have a significant role in diabetes. The present study is designed to probe the immunological response of native and glycated low-density lipoprotein (LDL) in experimental animals. The second part of this study is to probe glycoxidative lesion detection in low-density lipoproteins (LDL) in diabetes subjects with varying disease duration. The neo-epitopes attributed to glycation-induced glycoxidative lesion of LDL in DM patients’ plasma were, analyzed by binding of native and MG-modified LDL immunized animal sera antibodies using an immunochemical assay. The plasma purified human LDL glycation with MG, which instigated modification in LDL. Further, the NewZealand-White rabbits were infused with unmodified natural LDL (N-LDL) and MG-glycatedLDL to probe its immunogenicity. The glycoxidative lesion detection in LDL of DM with disease duration (D.D.) of 5–15 years and D.D. > 15 years was found to be significantly higher as compared to normal healthy subjects (NHS) LDL. The findings support the notion that prolonged duration of diabetes can cause structural alteration in LDL protein molecules, rendering them highly immunogenic in nature. The presence of LDL lesions specific to MG-associated glycoxidation would further help in assessing the progression of diabetes mellitus. MDPI 2023-09-29 /pmc/articles/PMC10608319/ /pubmed/37895368 http://dx.doi.org/10.3390/life13101986 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Alyahyawi, Amjad R. Khan, Mohd Yasir Alouffi, Sultan Maarfi, Farah Akasha, Rihab Khan, Saif Rafi, Zeeshan Alharazi, Talal Shahab, Uzma Ahmad, Saheem Identification of Glycoxidative Lesion in Isolated Low-Density Lipoproteins from Diabetes Mellitus Subjects |
title | Identification of Glycoxidative Lesion in Isolated Low-Density Lipoproteins from Diabetes Mellitus Subjects |
title_full | Identification of Glycoxidative Lesion in Isolated Low-Density Lipoproteins from Diabetes Mellitus Subjects |
title_fullStr | Identification of Glycoxidative Lesion in Isolated Low-Density Lipoproteins from Diabetes Mellitus Subjects |
title_full_unstemmed | Identification of Glycoxidative Lesion in Isolated Low-Density Lipoproteins from Diabetes Mellitus Subjects |
title_short | Identification of Glycoxidative Lesion in Isolated Low-Density Lipoproteins from Diabetes Mellitus Subjects |
title_sort | identification of glycoxidative lesion in isolated low-density lipoproteins from diabetes mellitus subjects |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10608319/ https://www.ncbi.nlm.nih.gov/pubmed/37895368 http://dx.doi.org/10.3390/life13101986 |
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