Cargando…

Streptococcal Arginine Deiminase Inhibits T Lymphocyte Differentiation In Vitro

Pathogenic microbes use arginine-metabolizing enzymes as an immune evasion strategy. In this study, the impact of streptococcal arginine deiminase (ADI) on the human peripheral blood T lymphocytes function in vitro was studied. The comparison of the effects of parental strain (Streptococcus pyogenes...

Descripción completa

Detalles Bibliográficos
Autores principales: Starikova, Eleonora A., Mammedova, Jennet T., Ozhiganova, Arina, Leveshko, Tatiana A., Lebedeva, Aleksandra M., Sokolov, Alexey V., Isakov, Dmitry V., Karaseva, Alena B., Burova, Larissa A., Kudryavtsev, Igor V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10608802/
https://www.ncbi.nlm.nih.gov/pubmed/37894243
http://dx.doi.org/10.3390/microorganisms11102585
_version_ 1785127863973838848
author Starikova, Eleonora A.
Mammedova, Jennet T.
Ozhiganova, Arina
Leveshko, Tatiana A.
Lebedeva, Aleksandra M.
Sokolov, Alexey V.
Isakov, Dmitry V.
Karaseva, Alena B.
Burova, Larissa A.
Kudryavtsev, Igor V.
author_facet Starikova, Eleonora A.
Mammedova, Jennet T.
Ozhiganova, Arina
Leveshko, Tatiana A.
Lebedeva, Aleksandra M.
Sokolov, Alexey V.
Isakov, Dmitry V.
Karaseva, Alena B.
Burova, Larissa A.
Kudryavtsev, Igor V.
author_sort Starikova, Eleonora A.
collection PubMed
description Pathogenic microbes use arginine-metabolizing enzymes as an immune evasion strategy. In this study, the impact of streptococcal arginine deiminase (ADI) on the human peripheral blood T lymphocytes function in vitro was studied. The comparison of the effects of parental strain (Streptococcus pyogenes M49-16) with wild type of ArcA gene and its isogenic mutant with inactivated ArcA gene (Streptococcus pyogenes M49-16delArcA) was carried out. It was found that ADI in parental strain SDSC composition resulted in a fivefold decrease in the arginine concentration in human peripheral blood mononuclear cell (PBMC) supernatants. Only parental strain SDSCs suppressed anti-CD2/CD3/CD28-bead-stimulated mitochondrial dehydrogenase activity and caused a twofold decrease in IL-2 production in PBMC. Flow cytometry analysis revealed that ADI decreased the percentage of CM (central memory) and increased the proportion of TEMRA (terminally differentiated effector memory) of CD4+ and CD8+ T cells subsets. Enzyme activity inhibited the proliferation of all CD8+ T cell subsets as well as CM, EM (effector memory), and TEMRA CD4+ T cells. One of the prominent ADI effects was the inhibition of autophagy processes in CD8+ CM and EM as well as CD4+ CM, EM, and TEMRA T cell subsets. The data obtained confirm arginine’s crucial role in controlling immune reactions and suggest that streptococcal ADI may downregulate adaptive immunity and immunological memory.
format Online
Article
Text
id pubmed-10608802
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-106088022023-10-28 Streptococcal Arginine Deiminase Inhibits T Lymphocyte Differentiation In Vitro Starikova, Eleonora A. Mammedova, Jennet T. Ozhiganova, Arina Leveshko, Tatiana A. Lebedeva, Aleksandra M. Sokolov, Alexey V. Isakov, Dmitry V. Karaseva, Alena B. Burova, Larissa A. Kudryavtsev, Igor V. Microorganisms Article Pathogenic microbes use arginine-metabolizing enzymes as an immune evasion strategy. In this study, the impact of streptococcal arginine deiminase (ADI) on the human peripheral blood T lymphocytes function in vitro was studied. The comparison of the effects of parental strain (Streptococcus pyogenes M49-16) with wild type of ArcA gene and its isogenic mutant with inactivated ArcA gene (Streptococcus pyogenes M49-16delArcA) was carried out. It was found that ADI in parental strain SDSC composition resulted in a fivefold decrease in the arginine concentration in human peripheral blood mononuclear cell (PBMC) supernatants. Only parental strain SDSCs suppressed anti-CD2/CD3/CD28-bead-stimulated mitochondrial dehydrogenase activity and caused a twofold decrease in IL-2 production in PBMC. Flow cytometry analysis revealed that ADI decreased the percentage of CM (central memory) and increased the proportion of TEMRA (terminally differentiated effector memory) of CD4+ and CD8+ T cells subsets. Enzyme activity inhibited the proliferation of all CD8+ T cell subsets as well as CM, EM (effector memory), and TEMRA CD4+ T cells. One of the prominent ADI effects was the inhibition of autophagy processes in CD8+ CM and EM as well as CD4+ CM, EM, and TEMRA T cell subsets. The data obtained confirm arginine’s crucial role in controlling immune reactions and suggest that streptococcal ADI may downregulate adaptive immunity and immunological memory. MDPI 2023-10-19 /pmc/articles/PMC10608802/ /pubmed/37894243 http://dx.doi.org/10.3390/microorganisms11102585 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Starikova, Eleonora A.
Mammedova, Jennet T.
Ozhiganova, Arina
Leveshko, Tatiana A.
Lebedeva, Aleksandra M.
Sokolov, Alexey V.
Isakov, Dmitry V.
Karaseva, Alena B.
Burova, Larissa A.
Kudryavtsev, Igor V.
Streptococcal Arginine Deiminase Inhibits T Lymphocyte Differentiation In Vitro
title Streptococcal Arginine Deiminase Inhibits T Lymphocyte Differentiation In Vitro
title_full Streptococcal Arginine Deiminase Inhibits T Lymphocyte Differentiation In Vitro
title_fullStr Streptococcal Arginine Deiminase Inhibits T Lymphocyte Differentiation In Vitro
title_full_unstemmed Streptococcal Arginine Deiminase Inhibits T Lymphocyte Differentiation In Vitro
title_short Streptococcal Arginine Deiminase Inhibits T Lymphocyte Differentiation In Vitro
title_sort streptococcal arginine deiminase inhibits t lymphocyte differentiation in vitro
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10608802/
https://www.ncbi.nlm.nih.gov/pubmed/37894243
http://dx.doi.org/10.3390/microorganisms11102585
work_keys_str_mv AT starikovaeleonoraa streptococcalargininedeiminaseinhibitstlymphocytedifferentiationinvitro
AT mammedovajennett streptococcalargininedeiminaseinhibitstlymphocytedifferentiationinvitro
AT ozhiganovaarina streptococcalargininedeiminaseinhibitstlymphocytedifferentiationinvitro
AT leveshkotatianaa streptococcalargininedeiminaseinhibitstlymphocytedifferentiationinvitro
AT lebedevaaleksandram streptococcalargininedeiminaseinhibitstlymphocytedifferentiationinvitro
AT sokolovalexeyv streptococcalargininedeiminaseinhibitstlymphocytedifferentiationinvitro
AT isakovdmitryv streptococcalargininedeiminaseinhibitstlymphocytedifferentiationinvitro
AT karasevaalenab streptococcalargininedeiminaseinhibitstlymphocytedifferentiationinvitro
AT burovalarissaa streptococcalargininedeiminaseinhibitstlymphocytedifferentiationinvitro
AT kudryavtsevigorv streptococcalargininedeiminaseinhibitstlymphocytedifferentiationinvitro