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Study of Ecological Relationship of Yeast Species with Candida albicans in the Context of Vulvovaginal Infections

The role of the fungal community, the mycobiota, in the health of the vagina is currently an important area of research. The emergence of new sequencing technologies and advances in bioinformatics made possible the discovery of novel fungi inhabiting this niche. Candida spp. constitutes the most imp...

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Autores principales: Caetano, Cátia Filipa, Gaspar, Carlos, Oliveira, Ana Sofia, Palmeira-de-Oliveira, Rita, Rodrigues, Lisa, Gonçalves, Teresa, Martinez-de-Oliveira, José, Palmeira-de-Oliveira, Ana, Rolo, Joana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10608876/
https://www.ncbi.nlm.nih.gov/pubmed/37894056
http://dx.doi.org/10.3390/microorganisms11102398
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author Caetano, Cátia Filipa
Gaspar, Carlos
Oliveira, Ana Sofia
Palmeira-de-Oliveira, Rita
Rodrigues, Lisa
Gonçalves, Teresa
Martinez-de-Oliveira, José
Palmeira-de-Oliveira, Ana
Rolo, Joana
author_facet Caetano, Cátia Filipa
Gaspar, Carlos
Oliveira, Ana Sofia
Palmeira-de-Oliveira, Rita
Rodrigues, Lisa
Gonçalves, Teresa
Martinez-de-Oliveira, José
Palmeira-de-Oliveira, Ana
Rolo, Joana
author_sort Caetano, Cátia Filipa
collection PubMed
description The role of the fungal community, the mycobiota, in the health of the vagina is currently an important area of research. The emergence of new sequencing technologies and advances in bioinformatics made possible the discovery of novel fungi inhabiting this niche. Candida spp. constitutes the most important group of opportunistic pathogenic fungi, being the most prevalent fungal species in vulvovaginal infections. However, fungi such as Rhodotorula spp., Naganishia spp. and Malassezia spp. have emerged as potential pathogens in this niche, and therefore it is clinically relevant to understand their ecological interaction with Candida spp. The main aim of this study was to evaluate the impact of yeasts on Candida albicans’ pathogenicity, focusing on in-vitro growth, and biofilm formation at different times of co-culture and germ tube formation. The assays were performed with isolated species or with co-cultures of C. albicans (ATCC10231) with one other yeast species: Rhodotorula mucilaginosa (DSM13621), Malassezia furfur (DSM6170) or Naganishia albida (DSM70215). The results showed that M. furfur creates a symbiotic relationship with C. albicans, enhancing the growth rate of the co-culture (149.69%), and of germ tube formation of C. albicans (119.8%) and inducing a higher amount of biofilm biomass of the co-culture, both when mixed (154.1%) and preformed (166.8%). As for the yeasts R. mucilaginosa and N. albida, the relationship is antagonistic (with a significant decrease in all assays), thus possibly repressing the mixture’s pathogenicity. These results shed light on the complex interactions between yeasts in the vaginal mycobiome.
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spelling pubmed-106088762023-10-28 Study of Ecological Relationship of Yeast Species with Candida albicans in the Context of Vulvovaginal Infections Caetano, Cátia Filipa Gaspar, Carlos Oliveira, Ana Sofia Palmeira-de-Oliveira, Rita Rodrigues, Lisa Gonçalves, Teresa Martinez-de-Oliveira, José Palmeira-de-Oliveira, Ana Rolo, Joana Microorganisms Article The role of the fungal community, the mycobiota, in the health of the vagina is currently an important area of research. The emergence of new sequencing technologies and advances in bioinformatics made possible the discovery of novel fungi inhabiting this niche. Candida spp. constitutes the most important group of opportunistic pathogenic fungi, being the most prevalent fungal species in vulvovaginal infections. However, fungi such as Rhodotorula spp., Naganishia spp. and Malassezia spp. have emerged as potential pathogens in this niche, and therefore it is clinically relevant to understand their ecological interaction with Candida spp. The main aim of this study was to evaluate the impact of yeasts on Candida albicans’ pathogenicity, focusing on in-vitro growth, and biofilm formation at different times of co-culture and germ tube formation. The assays were performed with isolated species or with co-cultures of C. albicans (ATCC10231) with one other yeast species: Rhodotorula mucilaginosa (DSM13621), Malassezia furfur (DSM6170) or Naganishia albida (DSM70215). The results showed that M. furfur creates a symbiotic relationship with C. albicans, enhancing the growth rate of the co-culture (149.69%), and of germ tube formation of C. albicans (119.8%) and inducing a higher amount of biofilm biomass of the co-culture, both when mixed (154.1%) and preformed (166.8%). As for the yeasts R. mucilaginosa and N. albida, the relationship is antagonistic (with a significant decrease in all assays), thus possibly repressing the mixture’s pathogenicity. These results shed light on the complex interactions between yeasts in the vaginal mycobiome. MDPI 2023-09-26 /pmc/articles/PMC10608876/ /pubmed/37894056 http://dx.doi.org/10.3390/microorganisms11102398 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Caetano, Cátia Filipa
Gaspar, Carlos
Oliveira, Ana Sofia
Palmeira-de-Oliveira, Rita
Rodrigues, Lisa
Gonçalves, Teresa
Martinez-de-Oliveira, José
Palmeira-de-Oliveira, Ana
Rolo, Joana
Study of Ecological Relationship of Yeast Species with Candida albicans in the Context of Vulvovaginal Infections
title Study of Ecological Relationship of Yeast Species with Candida albicans in the Context of Vulvovaginal Infections
title_full Study of Ecological Relationship of Yeast Species with Candida albicans in the Context of Vulvovaginal Infections
title_fullStr Study of Ecological Relationship of Yeast Species with Candida albicans in the Context of Vulvovaginal Infections
title_full_unstemmed Study of Ecological Relationship of Yeast Species with Candida albicans in the Context of Vulvovaginal Infections
title_short Study of Ecological Relationship of Yeast Species with Candida albicans in the Context of Vulvovaginal Infections
title_sort study of ecological relationship of yeast species with candida albicans in the context of vulvovaginal infections
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10608876/
https://www.ncbi.nlm.nih.gov/pubmed/37894056
http://dx.doi.org/10.3390/microorganisms11102398
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