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Application of CRISPR-Cas System to Mitigate Superbug Infections
Multidrug resistance in bacterial strains known as superbugs is estimated to cause fatal infections worldwide. Migration and urbanization have resulted in overcrowding and inadequate sanitation, contributing to a high risk of superbug infections within and between different communities. The CRISPR-C...
Autores principales: | , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10609045/ https://www.ncbi.nlm.nih.gov/pubmed/37894063 http://dx.doi.org/10.3390/microorganisms11102404 |
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author | Rabaan, Ali A. Al Fares, Mona A. Almaghaslah, Manar Alpakistany, Tariq Al Kaabi, Nawal A. Alshamrani, Saleh A. Alshehri, Ahmad A. Almazni, Ibrahim Abdullah Saif, Ahmed Hakami, Abdulrahim R. Khamis, Faryal Alfaresi, Mubarak Alsalem, Zainab Alsoliabi, Zainab A. Al Amri, Kawthar Amur Salim Hassoueh, Amal K. Mohapatra, Ranjan K. Arteaga-Livias, Kovy Alissa, Mohammed |
author_facet | Rabaan, Ali A. Al Fares, Mona A. Almaghaslah, Manar Alpakistany, Tariq Al Kaabi, Nawal A. Alshamrani, Saleh A. Alshehri, Ahmad A. Almazni, Ibrahim Abdullah Saif, Ahmed Hakami, Abdulrahim R. Khamis, Faryal Alfaresi, Mubarak Alsalem, Zainab Alsoliabi, Zainab A. Al Amri, Kawthar Amur Salim Hassoueh, Amal K. Mohapatra, Ranjan K. Arteaga-Livias, Kovy Alissa, Mohammed |
author_sort | Rabaan, Ali A. |
collection | PubMed |
description | Multidrug resistance in bacterial strains known as superbugs is estimated to cause fatal infections worldwide. Migration and urbanization have resulted in overcrowding and inadequate sanitation, contributing to a high risk of superbug infections within and between different communities. The CRISPR-Cas system, mainly type II, has been projected as a robust tool to precisely edit drug-resistant bacterial genomes to combat antibiotic-resistant bacterial strains effectively. To entirely opt for its potential, advanced development in the CRISPR-Cas system is needed to reduce toxicity and promote efficacy in gene-editing applications. This might involve base-editing techniques used to produce point mutations. These methods employ designed Cas9 variations, such as the adenine base editor (ABE) and the cytidine base editor (CBE), to directly edit single base pairs without causing DSBs. The CBE and ABE could change a target base pair into a different one (for example, G-C to A-T or C-G to A-T). In this review, we addressed the limitations of the CRISPR/Cas system and explored strategies for circumventing these limitations by applying diverse base-editing techniques. Furthermore, we also discussed recent research showcasing the ability of base editors to eliminate drug-resistant microbes. |
format | Online Article Text |
id | pubmed-10609045 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-106090452023-10-28 Application of CRISPR-Cas System to Mitigate Superbug Infections Rabaan, Ali A. Al Fares, Mona A. Almaghaslah, Manar Alpakistany, Tariq Al Kaabi, Nawal A. Alshamrani, Saleh A. Alshehri, Ahmad A. Almazni, Ibrahim Abdullah Saif, Ahmed Hakami, Abdulrahim R. Khamis, Faryal Alfaresi, Mubarak Alsalem, Zainab Alsoliabi, Zainab A. Al Amri, Kawthar Amur Salim Hassoueh, Amal K. Mohapatra, Ranjan K. Arteaga-Livias, Kovy Alissa, Mohammed Microorganisms Review Multidrug resistance in bacterial strains known as superbugs is estimated to cause fatal infections worldwide. Migration and urbanization have resulted in overcrowding and inadequate sanitation, contributing to a high risk of superbug infections within and between different communities. The CRISPR-Cas system, mainly type II, has been projected as a robust tool to precisely edit drug-resistant bacterial genomes to combat antibiotic-resistant bacterial strains effectively. To entirely opt for its potential, advanced development in the CRISPR-Cas system is needed to reduce toxicity and promote efficacy in gene-editing applications. This might involve base-editing techniques used to produce point mutations. These methods employ designed Cas9 variations, such as the adenine base editor (ABE) and the cytidine base editor (CBE), to directly edit single base pairs without causing DSBs. The CBE and ABE could change a target base pair into a different one (for example, G-C to A-T or C-G to A-T). In this review, we addressed the limitations of the CRISPR/Cas system and explored strategies for circumventing these limitations by applying diverse base-editing techniques. Furthermore, we also discussed recent research showcasing the ability of base editors to eliminate drug-resistant microbes. MDPI 2023-09-26 /pmc/articles/PMC10609045/ /pubmed/37894063 http://dx.doi.org/10.3390/microorganisms11102404 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Rabaan, Ali A. Al Fares, Mona A. Almaghaslah, Manar Alpakistany, Tariq Al Kaabi, Nawal A. Alshamrani, Saleh A. Alshehri, Ahmad A. Almazni, Ibrahim Abdullah Saif, Ahmed Hakami, Abdulrahim R. Khamis, Faryal Alfaresi, Mubarak Alsalem, Zainab Alsoliabi, Zainab A. Al Amri, Kawthar Amur Salim Hassoueh, Amal K. Mohapatra, Ranjan K. Arteaga-Livias, Kovy Alissa, Mohammed Application of CRISPR-Cas System to Mitigate Superbug Infections |
title | Application of CRISPR-Cas System to Mitigate Superbug Infections |
title_full | Application of CRISPR-Cas System to Mitigate Superbug Infections |
title_fullStr | Application of CRISPR-Cas System to Mitigate Superbug Infections |
title_full_unstemmed | Application of CRISPR-Cas System to Mitigate Superbug Infections |
title_short | Application of CRISPR-Cas System to Mitigate Superbug Infections |
title_sort | application of crispr-cas system to mitigate superbug infections |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10609045/ https://www.ncbi.nlm.nih.gov/pubmed/37894063 http://dx.doi.org/10.3390/microorganisms11102404 |
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