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Performance and Quality Comparison of Movie Alignment Software for Cryogenic Electron Microscopy
Cryogenic electron microscopy (Cryo-EM) has been established as one of the key players in structural biology. It can reconstruct a 3D model of a sample at a near-atomic resolution. With the increasing number of facilities, faster microscopes, and new imaging techniques, there is a growing demand for...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10609077/ https://www.ncbi.nlm.nih.gov/pubmed/37893272 http://dx.doi.org/10.3390/mi14101835 |
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author | Střelák, David Marchán, Daniel Carazo, José María S. Sorzano, Carlos O. |
author_facet | Střelák, David Marchán, Daniel Carazo, José María S. Sorzano, Carlos O. |
author_sort | Střelák, David |
collection | PubMed |
description | Cryogenic electron microscopy (Cryo-EM) has been established as one of the key players in structural biology. It can reconstruct a 3D model of a sample at a near-atomic resolution. With the increasing number of facilities, faster microscopes, and new imaging techniques, there is a growing demand for algorithms and programs able to process the so-called movie data produced by the microscopes in real time while preserving a high resolution and maximal information. In this article, we conduct a comparative analysis of the quality and performance of the most commonly used software for movie alignment. More precisely, we compare the most recent versions of FlexAlign (Xmipp v3.23.03), MotionCor2 (v1.6.4), Relion MotionCor (v4.0-beta), Warp (v1.0.9), and CryoSPARC (v4.0.3). We tested the quality of the alignment using generated phantom data, as well as real datasets, comparing the alignment precision, power spectra density, and performance scaling of each program. |
format | Online Article Text |
id | pubmed-10609077 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-106090772023-10-28 Performance and Quality Comparison of Movie Alignment Software for Cryogenic Electron Microscopy Střelák, David Marchán, Daniel Carazo, José María S. Sorzano, Carlos O. Micromachines (Basel) Article Cryogenic electron microscopy (Cryo-EM) has been established as one of the key players in structural biology. It can reconstruct a 3D model of a sample at a near-atomic resolution. With the increasing number of facilities, faster microscopes, and new imaging techniques, there is a growing demand for algorithms and programs able to process the so-called movie data produced by the microscopes in real time while preserving a high resolution and maximal information. In this article, we conduct a comparative analysis of the quality and performance of the most commonly used software for movie alignment. More precisely, we compare the most recent versions of FlexAlign (Xmipp v3.23.03), MotionCor2 (v1.6.4), Relion MotionCor (v4.0-beta), Warp (v1.0.9), and CryoSPARC (v4.0.3). We tested the quality of the alignment using generated phantom data, as well as real datasets, comparing the alignment precision, power spectra density, and performance scaling of each program. MDPI 2023-09-26 /pmc/articles/PMC10609077/ /pubmed/37893272 http://dx.doi.org/10.3390/mi14101835 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Střelák, David Marchán, Daniel Carazo, José María S. Sorzano, Carlos O. Performance and Quality Comparison of Movie Alignment Software for Cryogenic Electron Microscopy |
title | Performance and Quality Comparison of Movie Alignment Software for Cryogenic Electron Microscopy |
title_full | Performance and Quality Comparison of Movie Alignment Software for Cryogenic Electron Microscopy |
title_fullStr | Performance and Quality Comparison of Movie Alignment Software for Cryogenic Electron Microscopy |
title_full_unstemmed | Performance and Quality Comparison of Movie Alignment Software for Cryogenic Electron Microscopy |
title_short | Performance and Quality Comparison of Movie Alignment Software for Cryogenic Electron Microscopy |
title_sort | performance and quality comparison of movie alignment software for cryogenic electron microscopy |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10609077/ https://www.ncbi.nlm.nih.gov/pubmed/37893272 http://dx.doi.org/10.3390/mi14101835 |
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