Cargando…
In Vivo Production, Development and Storage of Oscheius myriophila (Nematoda: Rhabditida) in Galleria mellonella (Lepidoptera: Pyralidae)
Entomopathogenic nematodes have been used in biological control for some time and are an alternative for the control of insect pests, but during their implementation, situations have arisen that can be improved. These vary with each species and include their production and storage. Oscheius myriophi...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10609108/ https://www.ncbi.nlm.nih.gov/pubmed/37894229 http://dx.doi.org/10.3390/microorganisms11102571 |
_version_ | 1785127935986892800 |
---|---|
author | Guadarrama-Avila, Tania Marel Ramírez-Trujillo, José Augusto Rodríguez-Ocampo, Thania Gisel Peña-Chora, Guadalupe Arenas-Sosa, Iván Hernández-Velázquez, Víctor Manuel |
author_facet | Guadarrama-Avila, Tania Marel Ramírez-Trujillo, José Augusto Rodríguez-Ocampo, Thania Gisel Peña-Chora, Guadalupe Arenas-Sosa, Iván Hernández-Velázquez, Víctor Manuel |
author_sort | Guadarrama-Avila, Tania Marel |
collection | PubMed |
description | Entomopathogenic nematodes have been used in biological control for some time and are an alternative for the control of insect pests, but during their implementation, situations have arisen that can be improved. These vary with each species and include their production and storage. Oscheius myriophila, an entomopathogenic nematode (EPN), was monitored for its performance when produced in vivo, as well as its development using Galleria mellonella larvae, using the MC5-2014 strain isolated from soil samples in the municipality of Tepalcingo, Morelos, México. For a study with native strains of EPNs, a wide range of tests must be conducted because the required conditions can be very specific. In vivo production was quantified at initial infective juvenile (IJ) inocula of 50, 100 and 500, and we obtained the same production for the three inocula. The life cycle of the EPNs lasted 12 days, and two generations were observed in which adults were found at days 5 and 9. Both evaluations were performed at a temperature of 27 °C in G. mellonella larvae. In addition, the temperatures of 8, 12, 20 and 24 °C were evaluated for their storage, and we observed that the EPNs can be kept for at least 6 months, maintaining a survival rate of 58.67% and a good infective capacity at a temperature of 12 °C, remaining above 60%. |
format | Online Article Text |
id | pubmed-10609108 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-106091082023-10-28 In Vivo Production, Development and Storage of Oscheius myriophila (Nematoda: Rhabditida) in Galleria mellonella (Lepidoptera: Pyralidae) Guadarrama-Avila, Tania Marel Ramírez-Trujillo, José Augusto Rodríguez-Ocampo, Thania Gisel Peña-Chora, Guadalupe Arenas-Sosa, Iván Hernández-Velázquez, Víctor Manuel Microorganisms Article Entomopathogenic nematodes have been used in biological control for some time and are an alternative for the control of insect pests, but during their implementation, situations have arisen that can be improved. These vary with each species and include their production and storage. Oscheius myriophila, an entomopathogenic nematode (EPN), was monitored for its performance when produced in vivo, as well as its development using Galleria mellonella larvae, using the MC5-2014 strain isolated from soil samples in the municipality of Tepalcingo, Morelos, México. For a study with native strains of EPNs, a wide range of tests must be conducted because the required conditions can be very specific. In vivo production was quantified at initial infective juvenile (IJ) inocula of 50, 100 and 500, and we obtained the same production for the three inocula. The life cycle of the EPNs lasted 12 days, and two generations were observed in which adults were found at days 5 and 9. Both evaluations were performed at a temperature of 27 °C in G. mellonella larvae. In addition, the temperatures of 8, 12, 20 and 24 °C were evaluated for their storage, and we observed that the EPNs can be kept for at least 6 months, maintaining a survival rate of 58.67% and a good infective capacity at a temperature of 12 °C, remaining above 60%. MDPI 2023-10-16 /pmc/articles/PMC10609108/ /pubmed/37894229 http://dx.doi.org/10.3390/microorganisms11102571 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Guadarrama-Avila, Tania Marel Ramírez-Trujillo, José Augusto Rodríguez-Ocampo, Thania Gisel Peña-Chora, Guadalupe Arenas-Sosa, Iván Hernández-Velázquez, Víctor Manuel In Vivo Production, Development and Storage of Oscheius myriophila (Nematoda: Rhabditida) in Galleria mellonella (Lepidoptera: Pyralidae) |
title | In Vivo Production, Development and Storage of Oscheius myriophila (Nematoda: Rhabditida) in Galleria mellonella (Lepidoptera: Pyralidae) |
title_full | In Vivo Production, Development and Storage of Oscheius myriophila (Nematoda: Rhabditida) in Galleria mellonella (Lepidoptera: Pyralidae) |
title_fullStr | In Vivo Production, Development and Storage of Oscheius myriophila (Nematoda: Rhabditida) in Galleria mellonella (Lepidoptera: Pyralidae) |
title_full_unstemmed | In Vivo Production, Development and Storage of Oscheius myriophila (Nematoda: Rhabditida) in Galleria mellonella (Lepidoptera: Pyralidae) |
title_short | In Vivo Production, Development and Storage of Oscheius myriophila (Nematoda: Rhabditida) in Galleria mellonella (Lepidoptera: Pyralidae) |
title_sort | in vivo production, development and storage of oscheius myriophila (nematoda: rhabditida) in galleria mellonella (lepidoptera: pyralidae) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10609108/ https://www.ncbi.nlm.nih.gov/pubmed/37894229 http://dx.doi.org/10.3390/microorganisms11102571 |
work_keys_str_mv | AT guadarramaavilataniamarel invivoproductiondevelopmentandstorageofoscheiusmyriophilanematodarhabditidaingalleriamellonellalepidopterapyralidae AT ramireztrujillojoseaugusto invivoproductiondevelopmentandstorageofoscheiusmyriophilanematodarhabditidaingalleriamellonellalepidopterapyralidae AT rodriguezocampothaniagisel invivoproductiondevelopmentandstorageofoscheiusmyriophilanematodarhabditidaingalleriamellonellalepidopterapyralidae AT penachoraguadalupe invivoproductiondevelopmentandstorageofoscheiusmyriophilanematodarhabditidaingalleriamellonellalepidopterapyralidae AT arenassosaivan invivoproductiondevelopmentandstorageofoscheiusmyriophilanematodarhabditidaingalleriamellonellalepidopterapyralidae AT hernandezvelazquezvictormanuel invivoproductiondevelopmentandstorageofoscheiusmyriophilanematodarhabditidaingalleriamellonellalepidopterapyralidae |