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Spontaneous Aberrant Bodies Formation in Human Pneumocytes Infected with Estrella lausannensis
Estrella lausannensis, a Chlamydia-related bacterium isolated from a Spanish river, is considered as a possible emerging human pathogen. Indeed, it was recently demonstrated to multiply in human macrophages, resisting oxidative burst and causing a strong cytopathic effect. In addition, a preliminary...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10609161/ https://www.ncbi.nlm.nih.gov/pubmed/37894026 http://dx.doi.org/10.3390/microorganisms11102368 |
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author | Rovero, Aurelien Kebbi-Beghdadi, Carole Greub, Gilbert |
author_facet | Rovero, Aurelien Kebbi-Beghdadi, Carole Greub, Gilbert |
author_sort | Rovero, Aurelien |
collection | PubMed |
description | Estrella lausannensis, a Chlamydia-related bacterium isolated from a Spanish river, is considered as a possible emerging human pathogen. Indeed, it was recently demonstrated to multiply in human macrophages, resisting oxidative burst and causing a strong cytopathic effect. In addition, a preliminary study highlighted a correlation between antibody response to E. lausannensis and pneumonia in children. To clarify the pathogenic potential of these bacteria, we infected a human pneumocyte cell line with E. lausannensis and assessed its replication and cytopathic effect using quantitative real-time PCR and immunofluorescence, as well as confocal and electron microscopy. Our results demonstrated that E. lausannensis enters and replicates rapidly in human pneumocytes, and that it causes a prompt lysis of the host cells. Furthermore, we reported the spontaneous formation of aberrant bodies, a form associated with persistence in Chlamydiae, suggesting that E. lausannensis infection could cause chronic disorders in humans. |
format | Online Article Text |
id | pubmed-10609161 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-106091612023-10-28 Spontaneous Aberrant Bodies Formation in Human Pneumocytes Infected with Estrella lausannensis Rovero, Aurelien Kebbi-Beghdadi, Carole Greub, Gilbert Microorganisms Communication Estrella lausannensis, a Chlamydia-related bacterium isolated from a Spanish river, is considered as a possible emerging human pathogen. Indeed, it was recently demonstrated to multiply in human macrophages, resisting oxidative burst and causing a strong cytopathic effect. In addition, a preliminary study highlighted a correlation between antibody response to E. lausannensis and pneumonia in children. To clarify the pathogenic potential of these bacteria, we infected a human pneumocyte cell line with E. lausannensis and assessed its replication and cytopathic effect using quantitative real-time PCR and immunofluorescence, as well as confocal and electron microscopy. Our results demonstrated that E. lausannensis enters and replicates rapidly in human pneumocytes, and that it causes a prompt lysis of the host cells. Furthermore, we reported the spontaneous formation of aberrant bodies, a form associated with persistence in Chlamydiae, suggesting that E. lausannensis infection could cause chronic disorders in humans. MDPI 2023-09-22 /pmc/articles/PMC10609161/ /pubmed/37894026 http://dx.doi.org/10.3390/microorganisms11102368 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Rovero, Aurelien Kebbi-Beghdadi, Carole Greub, Gilbert Spontaneous Aberrant Bodies Formation in Human Pneumocytes Infected with Estrella lausannensis |
title | Spontaneous Aberrant Bodies Formation in Human Pneumocytes Infected with Estrella lausannensis |
title_full | Spontaneous Aberrant Bodies Formation in Human Pneumocytes Infected with Estrella lausannensis |
title_fullStr | Spontaneous Aberrant Bodies Formation in Human Pneumocytes Infected with Estrella lausannensis |
title_full_unstemmed | Spontaneous Aberrant Bodies Formation in Human Pneumocytes Infected with Estrella lausannensis |
title_short | Spontaneous Aberrant Bodies Formation in Human Pneumocytes Infected with Estrella lausannensis |
title_sort | spontaneous aberrant bodies formation in human pneumocytes infected with estrella lausannensis |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10609161/ https://www.ncbi.nlm.nih.gov/pubmed/37894026 http://dx.doi.org/10.3390/microorganisms11102368 |
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