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Spontaneous Aberrant Bodies Formation in Human Pneumocytes Infected with Estrella lausannensis

Estrella lausannensis, a Chlamydia-related bacterium isolated from a Spanish river, is considered as a possible emerging human pathogen. Indeed, it was recently demonstrated to multiply in human macrophages, resisting oxidative burst and causing a strong cytopathic effect. In addition, a preliminary...

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Autores principales: Rovero, Aurelien, Kebbi-Beghdadi, Carole, Greub, Gilbert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10609161/
https://www.ncbi.nlm.nih.gov/pubmed/37894026
http://dx.doi.org/10.3390/microorganisms11102368
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author Rovero, Aurelien
Kebbi-Beghdadi, Carole
Greub, Gilbert
author_facet Rovero, Aurelien
Kebbi-Beghdadi, Carole
Greub, Gilbert
author_sort Rovero, Aurelien
collection PubMed
description Estrella lausannensis, a Chlamydia-related bacterium isolated from a Spanish river, is considered as a possible emerging human pathogen. Indeed, it was recently demonstrated to multiply in human macrophages, resisting oxidative burst and causing a strong cytopathic effect. In addition, a preliminary study highlighted a correlation between antibody response to E. lausannensis and pneumonia in children. To clarify the pathogenic potential of these bacteria, we infected a human pneumocyte cell line with E. lausannensis and assessed its replication and cytopathic effect using quantitative real-time PCR and immunofluorescence, as well as confocal and electron microscopy. Our results demonstrated that E. lausannensis enters and replicates rapidly in human pneumocytes, and that it causes a prompt lysis of the host cells. Furthermore, we reported the spontaneous formation of aberrant bodies, a form associated with persistence in Chlamydiae, suggesting that E. lausannensis infection could cause chronic disorders in humans.
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spelling pubmed-106091612023-10-28 Spontaneous Aberrant Bodies Formation in Human Pneumocytes Infected with Estrella lausannensis Rovero, Aurelien Kebbi-Beghdadi, Carole Greub, Gilbert Microorganisms Communication Estrella lausannensis, a Chlamydia-related bacterium isolated from a Spanish river, is considered as a possible emerging human pathogen. Indeed, it was recently demonstrated to multiply in human macrophages, resisting oxidative burst and causing a strong cytopathic effect. In addition, a preliminary study highlighted a correlation between antibody response to E. lausannensis and pneumonia in children. To clarify the pathogenic potential of these bacteria, we infected a human pneumocyte cell line with E. lausannensis and assessed its replication and cytopathic effect using quantitative real-time PCR and immunofluorescence, as well as confocal and electron microscopy. Our results demonstrated that E. lausannensis enters and replicates rapidly in human pneumocytes, and that it causes a prompt lysis of the host cells. Furthermore, we reported the spontaneous formation of aberrant bodies, a form associated with persistence in Chlamydiae, suggesting that E. lausannensis infection could cause chronic disorders in humans. MDPI 2023-09-22 /pmc/articles/PMC10609161/ /pubmed/37894026 http://dx.doi.org/10.3390/microorganisms11102368 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Rovero, Aurelien
Kebbi-Beghdadi, Carole
Greub, Gilbert
Spontaneous Aberrant Bodies Formation in Human Pneumocytes Infected with Estrella lausannensis
title Spontaneous Aberrant Bodies Formation in Human Pneumocytes Infected with Estrella lausannensis
title_full Spontaneous Aberrant Bodies Formation in Human Pneumocytes Infected with Estrella lausannensis
title_fullStr Spontaneous Aberrant Bodies Formation in Human Pneumocytes Infected with Estrella lausannensis
title_full_unstemmed Spontaneous Aberrant Bodies Formation in Human Pneumocytes Infected with Estrella lausannensis
title_short Spontaneous Aberrant Bodies Formation in Human Pneumocytes Infected with Estrella lausannensis
title_sort spontaneous aberrant bodies formation in human pneumocytes infected with estrella lausannensis
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10609161/
https://www.ncbi.nlm.nih.gov/pubmed/37894026
http://dx.doi.org/10.3390/microorganisms11102368
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