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Relocating Glyceryl Trinitrate as an Anti-Virulence Agent against Pseudomonas aeruginosa and Serratia marcescens: Insights from Molecular and In Vivo Investigations
The problem of antibiotic resistance is a global critical public health concern. In light of the threat of returning to the pre-antibiotic era, new alternative approaches are required such as quorum-sensing (QS) disruption and virulence inhibition, both of which apply no discernible selective pressu...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10609227/ https://www.ncbi.nlm.nih.gov/pubmed/37894078 http://dx.doi.org/10.3390/microorganisms11102420 |
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author | Nazeih, Shaimaa I. Ali, Mohamed A. M. Halim, Alyaa S. Abdel Al-Lawati, Hanan Abbas, Hisham A. Al-Zharani, Mohammed Boufahja, Fehmi Alghamdi, Mashael A. Hegazy, Wael A. H. Seleem, Noura M. |
author_facet | Nazeih, Shaimaa I. Ali, Mohamed A. M. Halim, Alyaa S. Abdel Al-Lawati, Hanan Abbas, Hisham A. Al-Zharani, Mohammed Boufahja, Fehmi Alghamdi, Mashael A. Hegazy, Wael A. H. Seleem, Noura M. |
author_sort | Nazeih, Shaimaa I. |
collection | PubMed |
description | The problem of antibiotic resistance is a global critical public health concern. In light of the threat of returning to the pre-antibiotic era, new alternative approaches are required such as quorum-sensing (QS) disruption and virulence inhibition, both of which apply no discernible selective pressure on bacteria, therefore mitigating the potential for the development of resistant strains. Bearing in mind the significant role of QS in orchestrating bacterial virulence, disrupting QS becomes essential for effectively diminishing bacterial virulence. This study aimed to assess the potential use of sub-inhibitory concentration (0.25 mg/mL) of glyceryl trinitrate (GTN) to inhibit virulence in Serratia marcescens and Pseudomonas aeruginosa. GTN could decrease the expression of virulence genes in both tested bacteria in a significant manner. Histopathological study revealed the ability of GTN to alleviate the congestion in hepatic and renal tissues of infected mice and to reduce bacterial and leukocyte infiltration. This study recommends the use of topical GTN to treat topical infection caused by P. aeruginosa and S. marcescens in combination with antibiotics. |
format | Online Article Text |
id | pubmed-10609227 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-106092272023-10-28 Relocating Glyceryl Trinitrate as an Anti-Virulence Agent against Pseudomonas aeruginosa and Serratia marcescens: Insights from Molecular and In Vivo Investigations Nazeih, Shaimaa I. Ali, Mohamed A. M. Halim, Alyaa S. Abdel Al-Lawati, Hanan Abbas, Hisham A. Al-Zharani, Mohammed Boufahja, Fehmi Alghamdi, Mashael A. Hegazy, Wael A. H. Seleem, Noura M. Microorganisms Article The problem of antibiotic resistance is a global critical public health concern. In light of the threat of returning to the pre-antibiotic era, new alternative approaches are required such as quorum-sensing (QS) disruption and virulence inhibition, both of which apply no discernible selective pressure on bacteria, therefore mitigating the potential for the development of resistant strains. Bearing in mind the significant role of QS in orchestrating bacterial virulence, disrupting QS becomes essential for effectively diminishing bacterial virulence. This study aimed to assess the potential use of sub-inhibitory concentration (0.25 mg/mL) of glyceryl trinitrate (GTN) to inhibit virulence in Serratia marcescens and Pseudomonas aeruginosa. GTN could decrease the expression of virulence genes in both tested bacteria in a significant manner. Histopathological study revealed the ability of GTN to alleviate the congestion in hepatic and renal tissues of infected mice and to reduce bacterial and leukocyte infiltration. This study recommends the use of topical GTN to treat topical infection caused by P. aeruginosa and S. marcescens in combination with antibiotics. MDPI 2023-09-28 /pmc/articles/PMC10609227/ /pubmed/37894078 http://dx.doi.org/10.3390/microorganisms11102420 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Nazeih, Shaimaa I. Ali, Mohamed A. M. Halim, Alyaa S. Abdel Al-Lawati, Hanan Abbas, Hisham A. Al-Zharani, Mohammed Boufahja, Fehmi Alghamdi, Mashael A. Hegazy, Wael A. H. Seleem, Noura M. Relocating Glyceryl Trinitrate as an Anti-Virulence Agent against Pseudomonas aeruginosa and Serratia marcescens: Insights from Molecular and In Vivo Investigations |
title | Relocating Glyceryl Trinitrate as an Anti-Virulence Agent against Pseudomonas aeruginosa and Serratia marcescens: Insights from Molecular and In Vivo Investigations |
title_full | Relocating Glyceryl Trinitrate as an Anti-Virulence Agent against Pseudomonas aeruginosa and Serratia marcescens: Insights from Molecular and In Vivo Investigations |
title_fullStr | Relocating Glyceryl Trinitrate as an Anti-Virulence Agent against Pseudomonas aeruginosa and Serratia marcescens: Insights from Molecular and In Vivo Investigations |
title_full_unstemmed | Relocating Glyceryl Trinitrate as an Anti-Virulence Agent against Pseudomonas aeruginosa and Serratia marcescens: Insights from Molecular and In Vivo Investigations |
title_short | Relocating Glyceryl Trinitrate as an Anti-Virulence Agent against Pseudomonas aeruginosa and Serratia marcescens: Insights from Molecular and In Vivo Investigations |
title_sort | relocating glyceryl trinitrate as an anti-virulence agent against pseudomonas aeruginosa and serratia marcescens: insights from molecular and in vivo investigations |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10609227/ https://www.ncbi.nlm.nih.gov/pubmed/37894078 http://dx.doi.org/10.3390/microorganisms11102420 |
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