Cargando…

Effect of Flow Rate Modulation on Alginate Emulsification in Multistage Microfluidics

The encapsulation of stem cells into alginate microspheres is an important aspect of tissue engineering or bioprinting which ensures cell growth and development. We previously demonstrated the encapsulation of stem cells using the hanging drop method. However, this conventional process takes a relat...

Descripción completa

Detalles Bibliográficos
Autores principales: Whulanza, Yudan, Nathani, Rithwik Chandur, Adimillenva, Klaugusta, Irwansyah, Ridho, Wahyu Nurhayati, Retno, Utomo, Muhammad Satrio, Abdullah, Abdul Halim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10609249/
https://www.ncbi.nlm.nih.gov/pubmed/37893265
http://dx.doi.org/10.3390/mi14101828
_version_ 1785127969390329856
author Whulanza, Yudan
Nathani, Rithwik Chandur
Adimillenva, Klaugusta
Irwansyah, Ridho
Wahyu Nurhayati, Retno
Utomo, Muhammad Satrio
Abdullah, Abdul Halim
author_facet Whulanza, Yudan
Nathani, Rithwik Chandur
Adimillenva, Klaugusta
Irwansyah, Ridho
Wahyu Nurhayati, Retno
Utomo, Muhammad Satrio
Abdullah, Abdul Halim
author_sort Whulanza, Yudan
collection PubMed
description The encapsulation of stem cells into alginate microspheres is an important aspect of tissue engineering or bioprinting which ensures cell growth and development. We previously demonstrated the encapsulation of stem cells using the hanging drop method. However, this conventional process takes a relatively long time and only produces a small-volume droplet. Here, an experimental approach for alginate emulsification in multistage microfluidics is reported. By using the microfluidic method, the emulsification of alginate in oil can be manipulated by tuning the flow rate for both phases. Two-step droplet emulsification is conducted in a series of polycarbonate and polydimethylsiloxane microfluidic chips. Multistage emulsification of alginate for stem cell encapsulation has been successfully reported in this study under certain flow rates. Fundamental non-dimensional numbers such as Reynolds and capillary are used to evaluate the effect of flow rate on the emulsification process. Reynolds numbers of around 0.5–2.5 for alginate/water and 0.05–0.2 for oil phases were generated in the current study. The capillary number had a maximum value of 0.018 to ensure the formation of plug flow. By using the multistage emulsification system, the flow rates of each process can be tuned independently, offering a wider range of droplet sizes that can be produced. A final droplet size of 500–1000 µm can be produced using flow rates of 0.1–0.5 mL/h and 0.7–2.4 mL/h for the first stage and second stage, respectively.
format Online
Article
Text
id pubmed-10609249
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-106092492023-10-28 Effect of Flow Rate Modulation on Alginate Emulsification in Multistage Microfluidics Whulanza, Yudan Nathani, Rithwik Chandur Adimillenva, Klaugusta Irwansyah, Ridho Wahyu Nurhayati, Retno Utomo, Muhammad Satrio Abdullah, Abdul Halim Micromachines (Basel) Article The encapsulation of stem cells into alginate microspheres is an important aspect of tissue engineering or bioprinting which ensures cell growth and development. We previously demonstrated the encapsulation of stem cells using the hanging drop method. However, this conventional process takes a relatively long time and only produces a small-volume droplet. Here, an experimental approach for alginate emulsification in multistage microfluidics is reported. By using the microfluidic method, the emulsification of alginate in oil can be manipulated by tuning the flow rate for both phases. Two-step droplet emulsification is conducted in a series of polycarbonate and polydimethylsiloxane microfluidic chips. Multistage emulsification of alginate for stem cell encapsulation has been successfully reported in this study under certain flow rates. Fundamental non-dimensional numbers such as Reynolds and capillary are used to evaluate the effect of flow rate on the emulsification process. Reynolds numbers of around 0.5–2.5 for alginate/water and 0.05–0.2 for oil phases were generated in the current study. The capillary number had a maximum value of 0.018 to ensure the formation of plug flow. By using the multistage emulsification system, the flow rates of each process can be tuned independently, offering a wider range of droplet sizes that can be produced. A final droplet size of 500–1000 µm can be produced using flow rates of 0.1–0.5 mL/h and 0.7–2.4 mL/h for the first stage and second stage, respectively. MDPI 2023-09-26 /pmc/articles/PMC10609249/ /pubmed/37893265 http://dx.doi.org/10.3390/mi14101828 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Whulanza, Yudan
Nathani, Rithwik Chandur
Adimillenva, Klaugusta
Irwansyah, Ridho
Wahyu Nurhayati, Retno
Utomo, Muhammad Satrio
Abdullah, Abdul Halim
Effect of Flow Rate Modulation on Alginate Emulsification in Multistage Microfluidics
title Effect of Flow Rate Modulation on Alginate Emulsification in Multistage Microfluidics
title_full Effect of Flow Rate Modulation on Alginate Emulsification in Multistage Microfluidics
title_fullStr Effect of Flow Rate Modulation on Alginate Emulsification in Multistage Microfluidics
title_full_unstemmed Effect of Flow Rate Modulation on Alginate Emulsification in Multistage Microfluidics
title_short Effect of Flow Rate Modulation on Alginate Emulsification in Multistage Microfluidics
title_sort effect of flow rate modulation on alginate emulsification in multistage microfluidics
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10609249/
https://www.ncbi.nlm.nih.gov/pubmed/37893265
http://dx.doi.org/10.3390/mi14101828
work_keys_str_mv AT whulanzayudan effectofflowratemodulationonalginateemulsificationinmultistagemicrofluidics
AT nathanirithwikchandur effectofflowratemodulationonalginateemulsificationinmultistagemicrofluidics
AT adimillenvaklaugusta effectofflowratemodulationonalginateemulsificationinmultistagemicrofluidics
AT irwansyahridho effectofflowratemodulationonalginateemulsificationinmultistagemicrofluidics
AT wahyunurhayatiretno effectofflowratemodulationonalginateemulsificationinmultistagemicrofluidics
AT utomomuhammadsatrio effectofflowratemodulationonalginateemulsificationinmultistagemicrofluidics
AT abdullahabdulhalim effectofflowratemodulationonalginateemulsificationinmultistagemicrofluidics