Cargando…
Total Flavonoids from Camellia oleifera Alleviated Mycoplasma pneumoniae-Induced Lung Injury via Inhibition of the TLR2-Mediated NF-κB and MAPK Pathways
Mycoplasma pneumoniae (M. pneumoniae) is an atypical bacterial pathogen responsible for community-acquired pneumonia primarily among school-aged children and young adults. Camellia oleifera (C. oleifera) has been used as a medicinal and edible plant in China for centuries, the constituents from whic...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10609408/ https://www.ncbi.nlm.nih.gov/pubmed/37894556 http://dx.doi.org/10.3390/molecules28207077 |
_version_ | 1785128006691323904 |
---|---|
author | Ding, Nan Lei, Aihua Shi, Zhisheng Xiang, Lin Wei, Bo Wu, Yimou |
author_facet | Ding, Nan Lei, Aihua Shi, Zhisheng Xiang, Lin Wei, Bo Wu, Yimou |
author_sort | Ding, Nan |
collection | PubMed |
description | Mycoplasma pneumoniae (M. pneumoniae) is an atypical bacterial pathogen responsible for community-acquired pneumonia primarily among school-aged children and young adults. Camellia oleifera (C. oleifera) has been used as a medicinal and edible plant in China for centuries, the constituents from which possessed various bioactivities. Notably, flavonoids existing in residues of C. oleifera defatted seeds exhibited significant anti-inflammatory activities. In the present study, we investigated the impact of total flavonoids from C. oleifera (TFCO) seed extract on M. pneumoniae pneumonia. TFCO was obtained using multiple column chromatography methods and identified as kaempferol glycosides via UPLC-HRESIMS. In a M. pneumoniae pneumonia mouse model, TFCO significantly reduced the lung damage, suppressed IL-1β, IL-6, and TNF-α production, and curbed TLR2 activation triggered by M. pneumoniae. Similarly, in RAW264.7 macrophage cells stimulated by lipid-associated membrane proteins (LAMPs), TFCO suppressed the generation of proinflammatory cytokines and TLR2 expression. Moreover, TFCO diminished the phosphorylation of IκBα, JNK, ERK, p38, and p65 nuclear translocation in vitro. In conclusion, TFCO alleviated M. pneumoniae-induced lung damage via inhibition of TLR2-mediated NF-κB and MAPK pathways, suggesting its potential therapeutic application in M. pneumoniae-triggered lung inflammation. |
format | Online Article Text |
id | pubmed-10609408 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-106094082023-10-28 Total Flavonoids from Camellia oleifera Alleviated Mycoplasma pneumoniae-Induced Lung Injury via Inhibition of the TLR2-Mediated NF-κB and MAPK Pathways Ding, Nan Lei, Aihua Shi, Zhisheng Xiang, Lin Wei, Bo Wu, Yimou Molecules Article Mycoplasma pneumoniae (M. pneumoniae) is an atypical bacterial pathogen responsible for community-acquired pneumonia primarily among school-aged children and young adults. Camellia oleifera (C. oleifera) has been used as a medicinal and edible plant in China for centuries, the constituents from which possessed various bioactivities. Notably, flavonoids existing in residues of C. oleifera defatted seeds exhibited significant anti-inflammatory activities. In the present study, we investigated the impact of total flavonoids from C. oleifera (TFCO) seed extract on M. pneumoniae pneumonia. TFCO was obtained using multiple column chromatography methods and identified as kaempferol glycosides via UPLC-HRESIMS. In a M. pneumoniae pneumonia mouse model, TFCO significantly reduced the lung damage, suppressed IL-1β, IL-6, and TNF-α production, and curbed TLR2 activation triggered by M. pneumoniae. Similarly, in RAW264.7 macrophage cells stimulated by lipid-associated membrane proteins (LAMPs), TFCO suppressed the generation of proinflammatory cytokines and TLR2 expression. Moreover, TFCO diminished the phosphorylation of IκBα, JNK, ERK, p38, and p65 nuclear translocation in vitro. In conclusion, TFCO alleviated M. pneumoniae-induced lung damage via inhibition of TLR2-mediated NF-κB and MAPK pathways, suggesting its potential therapeutic application in M. pneumoniae-triggered lung inflammation. MDPI 2023-10-13 /pmc/articles/PMC10609408/ /pubmed/37894556 http://dx.doi.org/10.3390/molecules28207077 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ding, Nan Lei, Aihua Shi, Zhisheng Xiang, Lin Wei, Bo Wu, Yimou Total Flavonoids from Camellia oleifera Alleviated Mycoplasma pneumoniae-Induced Lung Injury via Inhibition of the TLR2-Mediated NF-κB and MAPK Pathways |
title | Total Flavonoids from Camellia oleifera Alleviated Mycoplasma pneumoniae-Induced Lung Injury via Inhibition of the TLR2-Mediated NF-κB and MAPK Pathways |
title_full | Total Flavonoids from Camellia oleifera Alleviated Mycoplasma pneumoniae-Induced Lung Injury via Inhibition of the TLR2-Mediated NF-κB and MAPK Pathways |
title_fullStr | Total Flavonoids from Camellia oleifera Alleviated Mycoplasma pneumoniae-Induced Lung Injury via Inhibition of the TLR2-Mediated NF-κB and MAPK Pathways |
title_full_unstemmed | Total Flavonoids from Camellia oleifera Alleviated Mycoplasma pneumoniae-Induced Lung Injury via Inhibition of the TLR2-Mediated NF-κB and MAPK Pathways |
title_short | Total Flavonoids from Camellia oleifera Alleviated Mycoplasma pneumoniae-Induced Lung Injury via Inhibition of the TLR2-Mediated NF-κB and MAPK Pathways |
title_sort | total flavonoids from camellia oleifera alleviated mycoplasma pneumoniae-induced lung injury via inhibition of the tlr2-mediated nf-κb and mapk pathways |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10609408/ https://www.ncbi.nlm.nih.gov/pubmed/37894556 http://dx.doi.org/10.3390/molecules28207077 |
work_keys_str_mv | AT dingnan totalflavonoidsfromcamelliaoleiferaalleviatedmycoplasmapneumoniaeinducedlunginjuryviainhibitionofthetlr2mediatednfkbandmapkpathways AT leiaihua totalflavonoidsfromcamelliaoleiferaalleviatedmycoplasmapneumoniaeinducedlunginjuryviainhibitionofthetlr2mediatednfkbandmapkpathways AT shizhisheng totalflavonoidsfromcamelliaoleiferaalleviatedmycoplasmapneumoniaeinducedlunginjuryviainhibitionofthetlr2mediatednfkbandmapkpathways AT xianglin totalflavonoidsfromcamelliaoleiferaalleviatedmycoplasmapneumoniaeinducedlunginjuryviainhibitionofthetlr2mediatednfkbandmapkpathways AT weibo totalflavonoidsfromcamelliaoleiferaalleviatedmycoplasmapneumoniaeinducedlunginjuryviainhibitionofthetlr2mediatednfkbandmapkpathways AT wuyimou totalflavonoidsfromcamelliaoleiferaalleviatedmycoplasmapneumoniaeinducedlunginjuryviainhibitionofthetlr2mediatednfkbandmapkpathways |