Cargando…

Biodistribution of Mesenchymal Stromal Cells Labeled with [(89)Zr]Zr-Oxine in Local Radiation Injuries in Laboratory Animals

Background: Tracking the migration pathways of living cells after their introduction into a patient’s body is a topical issue in the field of cell therapy. Questions related to studying the possibility of long-term intravital biodistribution of mesenchymal stromal cells in the body currently remain...

Descripción completa

Detalles Bibliográficos
Autores principales: Astrelina, Tatiana A., Brunchukov, Vitaliy A., Kodina, Galina E., Bubenshchikov, Viktor B., Larenkov, Anton A., Lunev, Aleksandr S., Petrosova, Kristina A., Rastorgueva, Anna A., Kobzeva, Irina V., Usupzhanova, Daria Y., Nikitina, Victoria A., Malsagova, Kristina A., Kulikova, Ludmila I., Samoilov, Alexander S., Pustovoyt, Vasiliy I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10609482/
https://www.ncbi.nlm.nih.gov/pubmed/37894647
http://dx.doi.org/10.3390/molecules28207169
_version_ 1785128024217223168
author Astrelina, Tatiana A.
Brunchukov, Vitaliy A.
Kodina, Galina E.
Bubenshchikov, Viktor B.
Larenkov, Anton A.
Lunev, Aleksandr S.
Petrosova, Kristina A.
Rastorgueva, Anna A.
Kobzeva, Irina V.
Usupzhanova, Daria Y.
Nikitina, Victoria A.
Malsagova, Kristina A.
Kulikova, Ludmila I.
Samoilov, Alexander S.
Pustovoyt, Vasiliy I.
author_facet Astrelina, Tatiana A.
Brunchukov, Vitaliy A.
Kodina, Galina E.
Bubenshchikov, Viktor B.
Larenkov, Anton A.
Lunev, Aleksandr S.
Petrosova, Kristina A.
Rastorgueva, Anna A.
Kobzeva, Irina V.
Usupzhanova, Daria Y.
Nikitina, Victoria A.
Malsagova, Kristina A.
Kulikova, Ludmila I.
Samoilov, Alexander S.
Pustovoyt, Vasiliy I.
author_sort Astrelina, Tatiana A.
collection PubMed
description Background: Tracking the migration pathways of living cells after their introduction into a patient’s body is a topical issue in the field of cell therapy. Questions related to studying the possibility of long-term intravital biodistribution of mesenchymal stromal cells in the body currently remain open. Methods: Forty-nine laboratory animals were used in the study. Modeling of local radiation injuries was carried out, and the dynamics of the distribution of mesenchymal stromal cells labeled with [(89)Zr]Zr-oxine in the rat body were studied. Results: the obtained results of the labelled cell distribution allow us to assume that this procedure could be useful for visualization of local radiation injury using positron emission tomography. However, further research is needed to confirm this assumption. Conclusions: intravenous injection leads to the initial accumulation of cells in the lungs and their subsequent redistribution to the liver, spleen, and kidneys. When locally injected into tissues, mesenchymal stromal cells are not distributed systemically in significant quantities.
format Online
Article
Text
id pubmed-10609482
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-106094822023-10-28 Biodistribution of Mesenchymal Stromal Cells Labeled with [(89)Zr]Zr-Oxine in Local Radiation Injuries in Laboratory Animals Astrelina, Tatiana A. Brunchukov, Vitaliy A. Kodina, Galina E. Bubenshchikov, Viktor B. Larenkov, Anton A. Lunev, Aleksandr S. Petrosova, Kristina A. Rastorgueva, Anna A. Kobzeva, Irina V. Usupzhanova, Daria Y. Nikitina, Victoria A. Malsagova, Kristina A. Kulikova, Ludmila I. Samoilov, Alexander S. Pustovoyt, Vasiliy I. Molecules Article Background: Tracking the migration pathways of living cells after their introduction into a patient’s body is a topical issue in the field of cell therapy. Questions related to studying the possibility of long-term intravital biodistribution of mesenchymal stromal cells in the body currently remain open. Methods: Forty-nine laboratory animals were used in the study. Modeling of local radiation injuries was carried out, and the dynamics of the distribution of mesenchymal stromal cells labeled with [(89)Zr]Zr-oxine in the rat body were studied. Results: the obtained results of the labelled cell distribution allow us to assume that this procedure could be useful for visualization of local radiation injury using positron emission tomography. However, further research is needed to confirm this assumption. Conclusions: intravenous injection leads to the initial accumulation of cells in the lungs and their subsequent redistribution to the liver, spleen, and kidneys. When locally injected into tissues, mesenchymal stromal cells are not distributed systemically in significant quantities. MDPI 2023-10-19 /pmc/articles/PMC10609482/ /pubmed/37894647 http://dx.doi.org/10.3390/molecules28207169 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Astrelina, Tatiana A.
Brunchukov, Vitaliy A.
Kodina, Galina E.
Bubenshchikov, Viktor B.
Larenkov, Anton A.
Lunev, Aleksandr S.
Petrosova, Kristina A.
Rastorgueva, Anna A.
Kobzeva, Irina V.
Usupzhanova, Daria Y.
Nikitina, Victoria A.
Malsagova, Kristina A.
Kulikova, Ludmila I.
Samoilov, Alexander S.
Pustovoyt, Vasiliy I.
Biodistribution of Mesenchymal Stromal Cells Labeled with [(89)Zr]Zr-Oxine in Local Radiation Injuries in Laboratory Animals
title Biodistribution of Mesenchymal Stromal Cells Labeled with [(89)Zr]Zr-Oxine in Local Radiation Injuries in Laboratory Animals
title_full Biodistribution of Mesenchymal Stromal Cells Labeled with [(89)Zr]Zr-Oxine in Local Radiation Injuries in Laboratory Animals
title_fullStr Biodistribution of Mesenchymal Stromal Cells Labeled with [(89)Zr]Zr-Oxine in Local Radiation Injuries in Laboratory Animals
title_full_unstemmed Biodistribution of Mesenchymal Stromal Cells Labeled with [(89)Zr]Zr-Oxine in Local Radiation Injuries in Laboratory Animals
title_short Biodistribution of Mesenchymal Stromal Cells Labeled with [(89)Zr]Zr-Oxine in Local Radiation Injuries in Laboratory Animals
title_sort biodistribution of mesenchymal stromal cells labeled with [(89)zr]zr-oxine in local radiation injuries in laboratory animals
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10609482/
https://www.ncbi.nlm.nih.gov/pubmed/37894647
http://dx.doi.org/10.3390/molecules28207169
work_keys_str_mv AT astrelinatatianaa biodistributionofmesenchymalstromalcellslabeledwith89zrzroxineinlocalradiationinjuriesinlaboratoryanimals
AT brunchukovvitaliya biodistributionofmesenchymalstromalcellslabeledwith89zrzroxineinlocalradiationinjuriesinlaboratoryanimals
AT kodinagalinae biodistributionofmesenchymalstromalcellslabeledwith89zrzroxineinlocalradiationinjuriesinlaboratoryanimals
AT bubenshchikovviktorb biodistributionofmesenchymalstromalcellslabeledwith89zrzroxineinlocalradiationinjuriesinlaboratoryanimals
AT larenkovantona biodistributionofmesenchymalstromalcellslabeledwith89zrzroxineinlocalradiationinjuriesinlaboratoryanimals
AT lunevaleksandrs biodistributionofmesenchymalstromalcellslabeledwith89zrzroxineinlocalradiationinjuriesinlaboratoryanimals
AT petrosovakristinaa biodistributionofmesenchymalstromalcellslabeledwith89zrzroxineinlocalradiationinjuriesinlaboratoryanimals
AT rastorguevaannaa biodistributionofmesenchymalstromalcellslabeledwith89zrzroxineinlocalradiationinjuriesinlaboratoryanimals
AT kobzevairinav biodistributionofmesenchymalstromalcellslabeledwith89zrzroxineinlocalradiationinjuriesinlaboratoryanimals
AT usupzhanovadariay biodistributionofmesenchymalstromalcellslabeledwith89zrzroxineinlocalradiationinjuriesinlaboratoryanimals
AT nikitinavictoriaa biodistributionofmesenchymalstromalcellslabeledwith89zrzroxineinlocalradiationinjuriesinlaboratoryanimals
AT malsagovakristinaa biodistributionofmesenchymalstromalcellslabeledwith89zrzroxineinlocalradiationinjuriesinlaboratoryanimals
AT kulikovaludmilai biodistributionofmesenchymalstromalcellslabeledwith89zrzroxineinlocalradiationinjuriesinlaboratoryanimals
AT samoilovalexanders biodistributionofmesenchymalstromalcellslabeledwith89zrzroxineinlocalradiationinjuriesinlaboratoryanimals
AT pustovoytvasiliyi biodistributionofmesenchymalstromalcellslabeledwith89zrzroxineinlocalradiationinjuriesinlaboratoryanimals