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In Vitro Assessment of the Antidiabetic and Anti-Inflammatory Potential of Artemisia absinthium, Artemisia vulgaris and Trigonella foenum-graecum Extracts Processed Using Membrane Technologies

Recently, there has been increased interest in the discovery of new natural herbal remedies for treating diabetes and inflammatory diseases. In this context, this work analyzed the antidiabetic and anti-inflammatory potential of Artemisia absinthium, Artemisia vulgaris and Trigonella foenum-graecum...

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Autores principales: Neagu, Elena, Paun, Gabriela, Albu, Camelia, Apreutesei, Oana Teodora, Radu, Gabriel Lucian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10609499/
https://www.ncbi.nlm.nih.gov/pubmed/37894635
http://dx.doi.org/10.3390/molecules28207156
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author Neagu, Elena
Paun, Gabriela
Albu, Camelia
Apreutesei, Oana Teodora
Radu, Gabriel Lucian
author_facet Neagu, Elena
Paun, Gabriela
Albu, Camelia
Apreutesei, Oana Teodora
Radu, Gabriel Lucian
author_sort Neagu, Elena
collection PubMed
description Recently, there has been increased interest in the discovery of new natural herbal remedies for treating diabetes and inflammatory diseases. In this context, this work analyzed the antidiabetic and anti-inflammatory potential of Artemisia absinthium, Artemisia vulgaris and Trigonella foenum-graecum herbs, which have been studied less from this point of view. Therefore, extracts were prepared and processed using membrane technologies, micro- and ultrafiltration, to concentrate the biologically active principles. The polyphenol and flavone contents in the extracts were analyzed. The qualitative analysis of the polyphenolic compounds was performed via HPLC, identifying chlorogenic acid, rosmarinic acid and rutin in A. absinthium; chlorogenic acid, luteolin and rutin in A. vulgaris; and genistin in T. foenum-graecum. The antidiabetic activity of the extracts was analyzed by testing their ability to inhibit α-amylase and α-glucosidase, and the anti-inflammatory activity was analyzed by testing their ability to inhibit hyaluronidase and lipoxygenase. Thus, the concentrated extracts of T. foenum-graecum showed high inhibitory activity on a-amylase—IC(50) = 3.22 ± 0.3 μg/mL—(compared with acarbose—IC(50) = 3.5 ± 0.18 μg/mL) and high inhibitory activity on LOX—IC(50) = 19.69 ± 0.52 μg/mL (compared with all standards used). The concentrated extract of A. vulgaris showed increased α-amylase inhibition activity—IC(50) = 8.57 ± 2.31 μg/mL—compared to acarbose IC(50) = 3.5 ± 0.18 μg/mL. The concentrated extract of A. absinthium showed pronounced LOX inhibition activity—IC(50) = 19.71 ± 0.79 μg/mL—compared to ibuprofen—IC(50) = 20.19 ± 1.25 μg/mL.
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spelling pubmed-106094992023-10-28 In Vitro Assessment of the Antidiabetic and Anti-Inflammatory Potential of Artemisia absinthium, Artemisia vulgaris and Trigonella foenum-graecum Extracts Processed Using Membrane Technologies Neagu, Elena Paun, Gabriela Albu, Camelia Apreutesei, Oana Teodora Radu, Gabriel Lucian Molecules Article Recently, there has been increased interest in the discovery of new natural herbal remedies for treating diabetes and inflammatory diseases. In this context, this work analyzed the antidiabetic and anti-inflammatory potential of Artemisia absinthium, Artemisia vulgaris and Trigonella foenum-graecum herbs, which have been studied less from this point of view. Therefore, extracts were prepared and processed using membrane technologies, micro- and ultrafiltration, to concentrate the biologically active principles. The polyphenol and flavone contents in the extracts were analyzed. The qualitative analysis of the polyphenolic compounds was performed via HPLC, identifying chlorogenic acid, rosmarinic acid and rutin in A. absinthium; chlorogenic acid, luteolin and rutin in A. vulgaris; and genistin in T. foenum-graecum. The antidiabetic activity of the extracts was analyzed by testing their ability to inhibit α-amylase and α-glucosidase, and the anti-inflammatory activity was analyzed by testing their ability to inhibit hyaluronidase and lipoxygenase. Thus, the concentrated extracts of T. foenum-graecum showed high inhibitory activity on a-amylase—IC(50) = 3.22 ± 0.3 μg/mL—(compared with acarbose—IC(50) = 3.5 ± 0.18 μg/mL) and high inhibitory activity on LOX—IC(50) = 19.69 ± 0.52 μg/mL (compared with all standards used). The concentrated extract of A. vulgaris showed increased α-amylase inhibition activity—IC(50) = 8.57 ± 2.31 μg/mL—compared to acarbose IC(50) = 3.5 ± 0.18 μg/mL. The concentrated extract of A. absinthium showed pronounced LOX inhibition activity—IC(50) = 19.71 ± 0.79 μg/mL—compared to ibuprofen—IC(50) = 20.19 ± 1.25 μg/mL. MDPI 2023-10-18 /pmc/articles/PMC10609499/ /pubmed/37894635 http://dx.doi.org/10.3390/molecules28207156 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Neagu, Elena
Paun, Gabriela
Albu, Camelia
Apreutesei, Oana Teodora
Radu, Gabriel Lucian
In Vitro Assessment of the Antidiabetic and Anti-Inflammatory Potential of Artemisia absinthium, Artemisia vulgaris and Trigonella foenum-graecum Extracts Processed Using Membrane Technologies
title In Vitro Assessment of the Antidiabetic and Anti-Inflammatory Potential of Artemisia absinthium, Artemisia vulgaris and Trigonella foenum-graecum Extracts Processed Using Membrane Technologies
title_full In Vitro Assessment of the Antidiabetic and Anti-Inflammatory Potential of Artemisia absinthium, Artemisia vulgaris and Trigonella foenum-graecum Extracts Processed Using Membrane Technologies
title_fullStr In Vitro Assessment of the Antidiabetic and Anti-Inflammatory Potential of Artemisia absinthium, Artemisia vulgaris and Trigonella foenum-graecum Extracts Processed Using Membrane Technologies
title_full_unstemmed In Vitro Assessment of the Antidiabetic and Anti-Inflammatory Potential of Artemisia absinthium, Artemisia vulgaris and Trigonella foenum-graecum Extracts Processed Using Membrane Technologies
title_short In Vitro Assessment of the Antidiabetic and Anti-Inflammatory Potential of Artemisia absinthium, Artemisia vulgaris and Trigonella foenum-graecum Extracts Processed Using Membrane Technologies
title_sort in vitro assessment of the antidiabetic and anti-inflammatory potential of artemisia absinthium, artemisia vulgaris and trigonella foenum-graecum extracts processed using membrane technologies
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10609499/
https://www.ncbi.nlm.nih.gov/pubmed/37894635
http://dx.doi.org/10.3390/molecules28207156
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