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Effects of systemic Anatolian propolis administration on a rat-irradiated osteoradionecrosis model
OBJECTIVE: Radiotherapy after head and neck cancer is associated with the risk of osteonecrosis development. This study aims to investigate the effectiveness of systemic propolis application to prevent the disease as it has no definite treatment protocol despite the proposed treatment methods and si...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Faculdade De Odontologia De Bauru - USP
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10609641/ https://www.ncbi.nlm.nih.gov/pubmed/37909529 http://dx.doi.org/10.1590/1678-7757-2023-0231 |
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author | ÇOLAK, Sefa ERDIL, Aras GEVREK, Fikret |
author_facet | ÇOLAK, Sefa ERDIL, Aras GEVREK, Fikret |
author_sort | ÇOLAK, Sefa |
collection | PubMed |
description | OBJECTIVE: Radiotherapy after head and neck cancer is associated with the risk of osteonecrosis development. This study aims to investigate the effectiveness of systemic propolis application to prevent the disease as it has no definite treatment protocol despite the proposed treatment methods and significantly decreases individuals’ quality of life. METHODOLOGY: In total, 29 male Wistar-Albino rats were divided into control, 35 Gy irradiation (Group 1), 35 Gy irradiation+100 mg/kg/ml propolis administration (Group 2), and 35 Gy irradiation+200 mg/kg/ml propolis administration groups (Group 3). Propolis was first applied on the day after radiotherapy, except for the control group. Right first and second molars were extracted from all rats three weeks following radiotherapy. Samples were collected seven weeks after radiotherapy. Osteoblast and osteoclast counts were calculated by histomorphometric analysis. Immunohistochemical analysis determined bone morphogenic protein-2 (BMP-2) and transforming growth factor beta-3 (TGFβ-3). RESULTS: Group comparison found non-significant differences regarding osteoblast (p=0.130) and osteoclast (p=0.063) counts. However, Group 1 showed the lowest mean osteoblast (OBL: 82.63 [±13.10]) and highest mean osteoclast counts (OCL: 12.63 [±5.55]). OBL/OCL ratio showed significant differences between groups (p=0.011). Despite the significant difference between the Control and Groups 1 (p=0.006) and 2 (p=0.029), Group 3 showed a non-significant difference (p=0.091). For BMP-2 and TGFB3, the control group showed significant differences with the other two groups (p<0.001), except for Group 3. CONCLUSION: Anatolian propolis showed beneficial effects in a radiotherapy-mediated osteonecrosis model, highlighting its potential as a promising intervention. |
format | Online Article Text |
id | pubmed-10609641 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Faculdade De Odontologia De Bauru - USP |
record_format | MEDLINE/PubMed |
spelling | pubmed-106096412023-10-28 Effects of systemic Anatolian propolis administration on a rat-irradiated osteoradionecrosis model ÇOLAK, Sefa ERDIL, Aras GEVREK, Fikret J Appl Oral Sci Original Article OBJECTIVE: Radiotherapy after head and neck cancer is associated with the risk of osteonecrosis development. This study aims to investigate the effectiveness of systemic propolis application to prevent the disease as it has no definite treatment protocol despite the proposed treatment methods and significantly decreases individuals’ quality of life. METHODOLOGY: In total, 29 male Wistar-Albino rats were divided into control, 35 Gy irradiation (Group 1), 35 Gy irradiation+100 mg/kg/ml propolis administration (Group 2), and 35 Gy irradiation+200 mg/kg/ml propolis administration groups (Group 3). Propolis was first applied on the day after radiotherapy, except for the control group. Right first and second molars were extracted from all rats three weeks following radiotherapy. Samples were collected seven weeks after radiotherapy. Osteoblast and osteoclast counts were calculated by histomorphometric analysis. Immunohistochemical analysis determined bone morphogenic protein-2 (BMP-2) and transforming growth factor beta-3 (TGFβ-3). RESULTS: Group comparison found non-significant differences regarding osteoblast (p=0.130) and osteoclast (p=0.063) counts. However, Group 1 showed the lowest mean osteoblast (OBL: 82.63 [±13.10]) and highest mean osteoclast counts (OCL: 12.63 [±5.55]). OBL/OCL ratio showed significant differences between groups (p=0.011). Despite the significant difference between the Control and Groups 1 (p=0.006) and 2 (p=0.029), Group 3 showed a non-significant difference (p=0.091). For BMP-2 and TGFB3, the control group showed significant differences with the other two groups (p<0.001), except for Group 3. CONCLUSION: Anatolian propolis showed beneficial effects in a radiotherapy-mediated osteonecrosis model, highlighting its potential as a promising intervention. Faculdade De Odontologia De Bauru - USP 2023-10-27 /pmc/articles/PMC10609641/ /pubmed/37909529 http://dx.doi.org/10.1590/1678-7757-2023-0231 Text en https://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article ÇOLAK, Sefa ERDIL, Aras GEVREK, Fikret Effects of systemic Anatolian propolis administration on a rat-irradiated osteoradionecrosis model |
title | Effects of systemic Anatolian propolis administration on a rat-irradiated osteoradionecrosis model |
title_full | Effects of systemic Anatolian propolis administration on a rat-irradiated osteoradionecrosis model |
title_fullStr | Effects of systemic Anatolian propolis administration on a rat-irradiated osteoradionecrosis model |
title_full_unstemmed | Effects of systemic Anatolian propolis administration on a rat-irradiated osteoradionecrosis model |
title_short | Effects of systemic Anatolian propolis administration on a rat-irradiated osteoradionecrosis model |
title_sort | effects of systemic anatolian propolis administration on a rat-irradiated osteoradionecrosis model |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10609641/ https://www.ncbi.nlm.nih.gov/pubmed/37909529 http://dx.doi.org/10.1590/1678-7757-2023-0231 |
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