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Effects of systemic Anatolian propolis administration on a rat-irradiated osteoradionecrosis model

OBJECTIVE: Radiotherapy after head and neck cancer is associated with the risk of osteonecrosis development. This study aims to investigate the effectiveness of systemic propolis application to prevent the disease as it has no definite treatment protocol despite the proposed treatment methods and si...

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Autores principales: ÇOLAK, Sefa, ERDIL, Aras, GEVREK, Fikret
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Faculdade De Odontologia De Bauru - USP 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10609641/
https://www.ncbi.nlm.nih.gov/pubmed/37909529
http://dx.doi.org/10.1590/1678-7757-2023-0231
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author ÇOLAK, Sefa
ERDIL, Aras
GEVREK, Fikret
author_facet ÇOLAK, Sefa
ERDIL, Aras
GEVREK, Fikret
author_sort ÇOLAK, Sefa
collection PubMed
description OBJECTIVE: Radiotherapy after head and neck cancer is associated with the risk of osteonecrosis development. This study aims to investigate the effectiveness of systemic propolis application to prevent the disease as it has no definite treatment protocol despite the proposed treatment methods and significantly decreases individuals’ quality of life. METHODOLOGY: In total, 29 male Wistar-Albino rats were divided into control, 35 Gy irradiation (Group 1), 35 Gy irradiation+100 mg/kg/ml propolis administration (Group 2), and 35 Gy irradiation+200 mg/kg/ml propolis administration groups (Group 3). Propolis was first applied on the day after radiotherapy, except for the control group. Right first and second molars were extracted from all rats three weeks following radiotherapy. Samples were collected seven weeks after radiotherapy. Osteoblast and osteoclast counts were calculated by histomorphometric analysis. Immunohistochemical analysis determined bone morphogenic protein-2 (BMP-2) and transforming growth factor beta-3 (TGFβ-3). RESULTS: Group comparison found non-significant differences regarding osteoblast (p=0.130) and osteoclast (p=0.063) counts. However, Group 1 showed the lowest mean osteoblast (OBL: 82.63 [±13.10]) and highest mean osteoclast counts (OCL: 12.63 [±5.55]). OBL/OCL ratio showed significant differences between groups (p=0.011). Despite the significant difference between the Control and Groups 1 (p=0.006) and 2 (p=0.029), Group 3 showed a non-significant difference (p=0.091). For BMP-2 and TGFB3, the control group showed significant differences with the other two groups (p<0.001), except for Group 3. CONCLUSION: Anatolian propolis showed beneficial effects in a radiotherapy-mediated osteonecrosis model, highlighting its potential as a promising intervention.
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spelling pubmed-106096412023-10-28 Effects of systemic Anatolian propolis administration on a rat-irradiated osteoradionecrosis model ÇOLAK, Sefa ERDIL, Aras GEVREK, Fikret J Appl Oral Sci Original Article OBJECTIVE: Radiotherapy after head and neck cancer is associated with the risk of osteonecrosis development. This study aims to investigate the effectiveness of systemic propolis application to prevent the disease as it has no definite treatment protocol despite the proposed treatment methods and significantly decreases individuals’ quality of life. METHODOLOGY: In total, 29 male Wistar-Albino rats were divided into control, 35 Gy irradiation (Group 1), 35 Gy irradiation+100 mg/kg/ml propolis administration (Group 2), and 35 Gy irradiation+200 mg/kg/ml propolis administration groups (Group 3). Propolis was first applied on the day after radiotherapy, except for the control group. Right first and second molars were extracted from all rats three weeks following radiotherapy. Samples were collected seven weeks after radiotherapy. Osteoblast and osteoclast counts were calculated by histomorphometric analysis. Immunohistochemical analysis determined bone morphogenic protein-2 (BMP-2) and transforming growth factor beta-3 (TGFβ-3). RESULTS: Group comparison found non-significant differences regarding osteoblast (p=0.130) and osteoclast (p=0.063) counts. However, Group 1 showed the lowest mean osteoblast (OBL: 82.63 [±13.10]) and highest mean osteoclast counts (OCL: 12.63 [±5.55]). OBL/OCL ratio showed significant differences between groups (p=0.011). Despite the significant difference between the Control and Groups 1 (p=0.006) and 2 (p=0.029), Group 3 showed a non-significant difference (p=0.091). For BMP-2 and TGFB3, the control group showed significant differences with the other two groups (p<0.001), except for Group 3. CONCLUSION: Anatolian propolis showed beneficial effects in a radiotherapy-mediated osteonecrosis model, highlighting its potential as a promising intervention. Faculdade De Odontologia De Bauru - USP 2023-10-27 /pmc/articles/PMC10609641/ /pubmed/37909529 http://dx.doi.org/10.1590/1678-7757-2023-0231 Text en https://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
ÇOLAK, Sefa
ERDIL, Aras
GEVREK, Fikret
Effects of systemic Anatolian propolis administration on a rat-irradiated osteoradionecrosis model
title Effects of systemic Anatolian propolis administration on a rat-irradiated osteoradionecrosis model
title_full Effects of systemic Anatolian propolis administration on a rat-irradiated osteoradionecrosis model
title_fullStr Effects of systemic Anatolian propolis administration on a rat-irradiated osteoradionecrosis model
title_full_unstemmed Effects of systemic Anatolian propolis administration on a rat-irradiated osteoradionecrosis model
title_short Effects of systemic Anatolian propolis administration on a rat-irradiated osteoradionecrosis model
title_sort effects of systemic anatolian propolis administration on a rat-irradiated osteoradionecrosis model
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10609641/
https://www.ncbi.nlm.nih.gov/pubmed/37909529
http://dx.doi.org/10.1590/1678-7757-2023-0231
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