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Chemoselective Coatings of GL13K Antimicrobial Peptides for Dental Implants

Dental implant−associated infection is a clinical challenge which poses a significant healthcare and socio−economic burden. To overcome this issue, developing antimicrobial surfaces, including antimicrobial peptide coatings, has gained great attention. Different physical and chemical routes have bee...

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Detalles Bibliográficos
Autores principales: Mutreja, Isha, Lan, Caixia, Li, Qishun, Aparicio, Conrado
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10609907/
https://www.ncbi.nlm.nih.gov/pubmed/37896178
http://dx.doi.org/10.3390/pharmaceutics15102418
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author Mutreja, Isha
Lan, Caixia
Li, Qishun
Aparicio, Conrado
author_facet Mutreja, Isha
Lan, Caixia
Li, Qishun
Aparicio, Conrado
author_sort Mutreja, Isha
collection PubMed
description Dental implant−associated infection is a clinical challenge which poses a significant healthcare and socio−economic burden. To overcome this issue, developing antimicrobial surfaces, including antimicrobial peptide coatings, has gained great attention. Different physical and chemical routes have been used to obtain these biofunctional coatings, which in turn might have a direct influence on their bioactivity and functionality. In this study, we present a silane−based, fast, and efficient chemoselective conjugation of antimicrobial peptides (Cys−GL13K) to coat titanium implant surfaces. Comprehensive surface analysis was performed to confirm the surface functionalization of as−prepared and mechanically challenged coatings. The antibacterial potency of the evaluated surfaces was confirmed against both Streptococcus gordonii and Streptococcus mutans, the primary colonizers and pathogens of dental surfaces, as demonstrated by reduced bacteria viability. Additionally, human dental pulp stem cells demonstrated long−term viability when cultured on Cys−GL13K−grafted titanium surfaces. Cell functionality and antimicrobial capability against multi−species need to be studied further; however, our results confirmed that the proposed chemistry for chemoselective peptide anchoring is a valid alternative to traditional site−unspecific anchoring methods and offers opportunities to modify varying biomaterial surfaces to form potent bioactive coatings with multiple functionalities to prevent infection.
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spelling pubmed-106099072023-10-28 Chemoselective Coatings of GL13K Antimicrobial Peptides for Dental Implants Mutreja, Isha Lan, Caixia Li, Qishun Aparicio, Conrado Pharmaceutics Article Dental implant−associated infection is a clinical challenge which poses a significant healthcare and socio−economic burden. To overcome this issue, developing antimicrobial surfaces, including antimicrobial peptide coatings, has gained great attention. Different physical and chemical routes have been used to obtain these biofunctional coatings, which in turn might have a direct influence on their bioactivity and functionality. In this study, we present a silane−based, fast, and efficient chemoselective conjugation of antimicrobial peptides (Cys−GL13K) to coat titanium implant surfaces. Comprehensive surface analysis was performed to confirm the surface functionalization of as−prepared and mechanically challenged coatings. The antibacterial potency of the evaluated surfaces was confirmed against both Streptococcus gordonii and Streptococcus mutans, the primary colonizers and pathogens of dental surfaces, as demonstrated by reduced bacteria viability. Additionally, human dental pulp stem cells demonstrated long−term viability when cultured on Cys−GL13K−grafted titanium surfaces. Cell functionality and antimicrobial capability against multi−species need to be studied further; however, our results confirmed that the proposed chemistry for chemoselective peptide anchoring is a valid alternative to traditional site−unspecific anchoring methods and offers opportunities to modify varying biomaterial surfaces to form potent bioactive coatings with multiple functionalities to prevent infection. MDPI 2023-10-04 /pmc/articles/PMC10609907/ /pubmed/37896178 http://dx.doi.org/10.3390/pharmaceutics15102418 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mutreja, Isha
Lan, Caixia
Li, Qishun
Aparicio, Conrado
Chemoselective Coatings of GL13K Antimicrobial Peptides for Dental Implants
title Chemoselective Coatings of GL13K Antimicrobial Peptides for Dental Implants
title_full Chemoselective Coatings of GL13K Antimicrobial Peptides for Dental Implants
title_fullStr Chemoselective Coatings of GL13K Antimicrobial Peptides for Dental Implants
title_full_unstemmed Chemoselective Coatings of GL13K Antimicrobial Peptides for Dental Implants
title_short Chemoselective Coatings of GL13K Antimicrobial Peptides for Dental Implants
title_sort chemoselective coatings of gl13k antimicrobial peptides for dental implants
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10609907/
https://www.ncbi.nlm.nih.gov/pubmed/37896178
http://dx.doi.org/10.3390/pharmaceutics15102418
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