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Investigation into the Relationship between Spermidine Degradation and Phenolic Compounds Biosynthesis in Barley Seedlings under Ultraviolet B Stress
Barley germination under ultraviolet B (UV-B) illumination stress induces effective accumulation of phenolic compounds in the barley. Spermidine can enhance the biosynthesis of phenolic compounds and alleviate the oxidative damage caused by UV-B. To better understand the function of spermidine, inhi...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10609958/ https://www.ncbi.nlm.nih.gov/pubmed/37895996 http://dx.doi.org/10.3390/plants12203533 |
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author | Xie, Chong Zhu, Yahui Leng, Chaoqun Wang, Qiaoe Wang, Pei Yang, Runqiang |
author_facet | Xie, Chong Zhu, Yahui Leng, Chaoqun Wang, Qiaoe Wang, Pei Yang, Runqiang |
author_sort | Xie, Chong |
collection | PubMed |
description | Barley germination under ultraviolet B (UV-B) illumination stress induces effective accumulation of phenolic compounds in the barley. Spermidine can enhance the biosynthesis of phenolic compounds and alleviate the oxidative damage caused by UV-B. To better understand the function of spermidine, inhibitors of enzymes that are involved in the degradation of spermidine and the synthesis of gamma-aminobutyric acid (GABA), the product of spermidine degradation, were applied to barley germinated under UV-B treatment. The results showed a more severe oxidative damage, and a decrease in phenolic acid contents were observed when spermidine degradation was inhibited. However, GABA application did attenuate an increase in electrolyte permeability and MDA content caused by UV-B induced oxidative damage and improved the respiration rate. Meanwhile, GABA application can elevate the accumulation of phenolic compounds by ca. 20%, by elevating the activities of some key enzymes. Furthermore, the application of GABA, together with the inhibitor of spermidine degradation, can alleviate its suppression of the synthesis of phenolic acids, and resistance to UV-B stress. In conclusion, spermidine alleviated oxidative damage and enhanced the accumulation of phenolic compounds using its degradation product. |
format | Online Article Text |
id | pubmed-10609958 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-106099582023-10-28 Investigation into the Relationship between Spermidine Degradation and Phenolic Compounds Biosynthesis in Barley Seedlings under Ultraviolet B Stress Xie, Chong Zhu, Yahui Leng, Chaoqun Wang, Qiaoe Wang, Pei Yang, Runqiang Plants (Basel) Article Barley germination under ultraviolet B (UV-B) illumination stress induces effective accumulation of phenolic compounds in the barley. Spermidine can enhance the biosynthesis of phenolic compounds and alleviate the oxidative damage caused by UV-B. To better understand the function of spermidine, inhibitors of enzymes that are involved in the degradation of spermidine and the synthesis of gamma-aminobutyric acid (GABA), the product of spermidine degradation, were applied to barley germinated under UV-B treatment. The results showed a more severe oxidative damage, and a decrease in phenolic acid contents were observed when spermidine degradation was inhibited. However, GABA application did attenuate an increase in electrolyte permeability and MDA content caused by UV-B induced oxidative damage and improved the respiration rate. Meanwhile, GABA application can elevate the accumulation of phenolic compounds by ca. 20%, by elevating the activities of some key enzymes. Furthermore, the application of GABA, together with the inhibitor of spermidine degradation, can alleviate its suppression of the synthesis of phenolic acids, and resistance to UV-B stress. In conclusion, spermidine alleviated oxidative damage and enhanced the accumulation of phenolic compounds using its degradation product. MDPI 2023-10-11 /pmc/articles/PMC10609958/ /pubmed/37895996 http://dx.doi.org/10.3390/plants12203533 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Xie, Chong Zhu, Yahui Leng, Chaoqun Wang, Qiaoe Wang, Pei Yang, Runqiang Investigation into the Relationship between Spermidine Degradation and Phenolic Compounds Biosynthesis in Barley Seedlings under Ultraviolet B Stress |
title | Investigation into the Relationship between Spermidine Degradation and Phenolic Compounds Biosynthesis in Barley Seedlings under Ultraviolet B Stress |
title_full | Investigation into the Relationship between Spermidine Degradation and Phenolic Compounds Biosynthesis in Barley Seedlings under Ultraviolet B Stress |
title_fullStr | Investigation into the Relationship between Spermidine Degradation and Phenolic Compounds Biosynthesis in Barley Seedlings under Ultraviolet B Stress |
title_full_unstemmed | Investigation into the Relationship between Spermidine Degradation and Phenolic Compounds Biosynthesis in Barley Seedlings under Ultraviolet B Stress |
title_short | Investigation into the Relationship between Spermidine Degradation and Phenolic Compounds Biosynthesis in Barley Seedlings under Ultraviolet B Stress |
title_sort | investigation into the relationship between spermidine degradation and phenolic compounds biosynthesis in barley seedlings under ultraviolet b stress |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10609958/ https://www.ncbi.nlm.nih.gov/pubmed/37895996 http://dx.doi.org/10.3390/plants12203533 |
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