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A Poly-D-lysine-Coated Coralline Matrix Promotes Hippocampal Neural Precursor Cells’ Differentiation into GFAP-Positive Astrocytes

A major goal of regenerative medicine of the central nervous system is to accelerate the regeneration of nerve tissue, where astrocytes, despite their positive and negative roles, play a critical role. Thus, scaffolds capable of producing astrocytes from neural precursor cells (NPCs) are most desira...

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Autores principales: Hendler, Roni Mina, Weiss, Orly Eva, Morad, Tzachy, Sion, Guy, Kirby, Michael, Dubinsky, Zvy, Barbora, Ayan, Minnes, Refael, Baranes, Danny
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10610048/
https://www.ncbi.nlm.nih.gov/pubmed/37896298
http://dx.doi.org/10.3390/polym15204054
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author Hendler, Roni Mina
Weiss, Orly Eva
Morad, Tzachy
Sion, Guy
Kirby, Michael
Dubinsky, Zvy
Barbora, Ayan
Minnes, Refael
Baranes, Danny
author_facet Hendler, Roni Mina
Weiss, Orly Eva
Morad, Tzachy
Sion, Guy
Kirby, Michael
Dubinsky, Zvy
Barbora, Ayan
Minnes, Refael
Baranes, Danny
author_sort Hendler, Roni Mina
collection PubMed
description A major goal of regenerative medicine of the central nervous system is to accelerate the regeneration of nerve tissue, where astrocytes, despite their positive and negative roles, play a critical role. Thus, scaffolds capable of producing astrocytes from neural precursor cells (NPCs) are most desirable. Our study shows that NPCs are converted into reactive astrocytes upon cultivation on coralline-derived calcium carbonate coated with poly-D-lysine (PDL-CS). As shown via nuclei staining, the adhesion of neurospheres containing hundreds of hippocampal neural cells to PDL-CS resulted in disaggregation of the cell cluster as well as the radial migration of dozens of cells away from the neurosphere core. Migrating cells per neurosphere averaged 100 on PDL-CS, significantly higher than on uncoated CS (28), PDL-coated glass (65), or uncoated glass (20). After 3 days of culture on PDL-CS, cell migration plateaued and remained stable for four more days. In addition, NPCs expressing nestin underwent continuous morphological changes from round to spiky, extending and elongating their processes, resembling activated astrocytes. The extension of the process increased continuously during the maturation of the culture and doubled after 7 days compared to day 1, whereas bifurcation increased by twofold during the first 3 days before plateauing. In addition, nestin positive cells’ shape, measured through the opposite circularity level correlation, decreased approximately twofold after three days, indicating spiky transformation. Moreover, nestin-positive cells co-expressing GFAP increased by 2.2 from day 1 to 7, reaching 40% of the NPC population on day 7. In this way, PDL-CS promotes NPC differentiation into reactive astrocytes, which could accelerate the repair of neural tissue.
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spelling pubmed-106100482023-10-28 A Poly-D-lysine-Coated Coralline Matrix Promotes Hippocampal Neural Precursor Cells’ Differentiation into GFAP-Positive Astrocytes Hendler, Roni Mina Weiss, Orly Eva Morad, Tzachy Sion, Guy Kirby, Michael Dubinsky, Zvy Barbora, Ayan Minnes, Refael Baranes, Danny Polymers (Basel) Article A major goal of regenerative medicine of the central nervous system is to accelerate the regeneration of nerve tissue, where astrocytes, despite their positive and negative roles, play a critical role. Thus, scaffolds capable of producing astrocytes from neural precursor cells (NPCs) are most desirable. Our study shows that NPCs are converted into reactive astrocytes upon cultivation on coralline-derived calcium carbonate coated with poly-D-lysine (PDL-CS). As shown via nuclei staining, the adhesion of neurospheres containing hundreds of hippocampal neural cells to PDL-CS resulted in disaggregation of the cell cluster as well as the radial migration of dozens of cells away from the neurosphere core. Migrating cells per neurosphere averaged 100 on PDL-CS, significantly higher than on uncoated CS (28), PDL-coated glass (65), or uncoated glass (20). After 3 days of culture on PDL-CS, cell migration plateaued and remained stable for four more days. In addition, NPCs expressing nestin underwent continuous morphological changes from round to spiky, extending and elongating their processes, resembling activated astrocytes. The extension of the process increased continuously during the maturation of the culture and doubled after 7 days compared to day 1, whereas bifurcation increased by twofold during the first 3 days before plateauing. In addition, nestin positive cells’ shape, measured through the opposite circularity level correlation, decreased approximately twofold after three days, indicating spiky transformation. Moreover, nestin-positive cells co-expressing GFAP increased by 2.2 from day 1 to 7, reaching 40% of the NPC population on day 7. In this way, PDL-CS promotes NPC differentiation into reactive astrocytes, which could accelerate the repair of neural tissue. MDPI 2023-10-11 /pmc/articles/PMC10610048/ /pubmed/37896298 http://dx.doi.org/10.3390/polym15204054 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hendler, Roni Mina
Weiss, Orly Eva
Morad, Tzachy
Sion, Guy
Kirby, Michael
Dubinsky, Zvy
Barbora, Ayan
Minnes, Refael
Baranes, Danny
A Poly-D-lysine-Coated Coralline Matrix Promotes Hippocampal Neural Precursor Cells’ Differentiation into GFAP-Positive Astrocytes
title A Poly-D-lysine-Coated Coralline Matrix Promotes Hippocampal Neural Precursor Cells’ Differentiation into GFAP-Positive Astrocytes
title_full A Poly-D-lysine-Coated Coralline Matrix Promotes Hippocampal Neural Precursor Cells’ Differentiation into GFAP-Positive Astrocytes
title_fullStr A Poly-D-lysine-Coated Coralline Matrix Promotes Hippocampal Neural Precursor Cells’ Differentiation into GFAP-Positive Astrocytes
title_full_unstemmed A Poly-D-lysine-Coated Coralline Matrix Promotes Hippocampal Neural Precursor Cells’ Differentiation into GFAP-Positive Astrocytes
title_short A Poly-D-lysine-Coated Coralline Matrix Promotes Hippocampal Neural Precursor Cells’ Differentiation into GFAP-Positive Astrocytes
title_sort poly-d-lysine-coated coralline matrix promotes hippocampal neural precursor cells’ differentiation into gfap-positive astrocytes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10610048/
https://www.ncbi.nlm.nih.gov/pubmed/37896298
http://dx.doi.org/10.3390/polym15204054
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