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Callus Type, Growth Regulators, and Phytagel on Indirect Somatic Embryogenesis of Coffee (Coffea arabica L. var. Colombia)

Coffee is a crop of global relevance. Indirect somatic embryogenesis has allowed plants of different coffee genotypes to be massively regenerated. The culture medium composition can affect the calli characteristics that are generated and their ability to form somatic embryos. This research aimed to...

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Autores principales: Avila-Victor, Consuelo Margarita, Arjona-Suárez, Enrique de Jesús, Iracheta-Donjuan, Leobardo, Valdez-Carrasco, Jorge Manuel, Gómez-Merino, Fernando Carlos, Robledo-Paz, Alejandrina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10610154/
https://www.ncbi.nlm.nih.gov/pubmed/37896033
http://dx.doi.org/10.3390/plants12203570
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author Avila-Victor, Consuelo Margarita
Arjona-Suárez, Enrique de Jesús
Iracheta-Donjuan, Leobardo
Valdez-Carrasco, Jorge Manuel
Gómez-Merino, Fernando Carlos
Robledo-Paz, Alejandrina
author_facet Avila-Victor, Consuelo Margarita
Arjona-Suárez, Enrique de Jesús
Iracheta-Donjuan, Leobardo
Valdez-Carrasco, Jorge Manuel
Gómez-Merino, Fernando Carlos
Robledo-Paz, Alejandrina
author_sort Avila-Victor, Consuelo Margarita
collection PubMed
description Coffee is a crop of global relevance. Indirect somatic embryogenesis has allowed plants of different coffee genotypes to be massively regenerated. The culture medium composition can affect the calli characteristics that are generated and their ability to form somatic embryos. This research aimed to determine the influence of the type of callus, growth regulators, and phytagel concentration on the embryogenic capacity of the Colombia variety. Leaf explants were cultured on Murashige and Skoog medium with 2,4-dichlorophenoxyacetic acid (2,4-D) (0.5–1.0 mg L(−1)), benzylaminopurine (BAP, 1.0 mg L(−1)), and phytagel (2.3–5.0 g L(−1)). The explants generated two types of calli: friable (beige, soft, watery, easy disintegration, polyhedral parenchyma cells) and compact (white, hard, low water content, difficult disintegration, elongated parenchyma cells). About 68% of the total callus generated was compact; this type of callus produced a greater number of embryos (71.3) than the friable one (29.2). The number of differentiated embryos was significantly affected by the concentration of phytagel; higher concentrations (5.0 g L(−1)) resulted in larger quantities (73.7). The highest number of embryos (127.47) was obtained by combining 1.0 mg L(−1) 2,4-D, 1.0 mg L(−1) BAP, 5.0 g L(−1) phytagel, and compact callus.
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spelling pubmed-106101542023-10-28 Callus Type, Growth Regulators, and Phytagel on Indirect Somatic Embryogenesis of Coffee (Coffea arabica L. var. Colombia) Avila-Victor, Consuelo Margarita Arjona-Suárez, Enrique de Jesús Iracheta-Donjuan, Leobardo Valdez-Carrasco, Jorge Manuel Gómez-Merino, Fernando Carlos Robledo-Paz, Alejandrina Plants (Basel) Article Coffee is a crop of global relevance. Indirect somatic embryogenesis has allowed plants of different coffee genotypes to be massively regenerated. The culture medium composition can affect the calli characteristics that are generated and their ability to form somatic embryos. This research aimed to determine the influence of the type of callus, growth regulators, and phytagel concentration on the embryogenic capacity of the Colombia variety. Leaf explants were cultured on Murashige and Skoog medium with 2,4-dichlorophenoxyacetic acid (2,4-D) (0.5–1.0 mg L(−1)), benzylaminopurine (BAP, 1.0 mg L(−1)), and phytagel (2.3–5.0 g L(−1)). The explants generated two types of calli: friable (beige, soft, watery, easy disintegration, polyhedral parenchyma cells) and compact (white, hard, low water content, difficult disintegration, elongated parenchyma cells). About 68% of the total callus generated was compact; this type of callus produced a greater number of embryos (71.3) than the friable one (29.2). The number of differentiated embryos was significantly affected by the concentration of phytagel; higher concentrations (5.0 g L(−1)) resulted in larger quantities (73.7). The highest number of embryos (127.47) was obtained by combining 1.0 mg L(−1) 2,4-D, 1.0 mg L(−1) BAP, 5.0 g L(−1) phytagel, and compact callus. MDPI 2023-10-14 /pmc/articles/PMC10610154/ /pubmed/37896033 http://dx.doi.org/10.3390/plants12203570 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Avila-Victor, Consuelo Margarita
Arjona-Suárez, Enrique de Jesús
Iracheta-Donjuan, Leobardo
Valdez-Carrasco, Jorge Manuel
Gómez-Merino, Fernando Carlos
Robledo-Paz, Alejandrina
Callus Type, Growth Regulators, and Phytagel on Indirect Somatic Embryogenesis of Coffee (Coffea arabica L. var. Colombia)
title Callus Type, Growth Regulators, and Phytagel on Indirect Somatic Embryogenesis of Coffee (Coffea arabica L. var. Colombia)
title_full Callus Type, Growth Regulators, and Phytagel on Indirect Somatic Embryogenesis of Coffee (Coffea arabica L. var. Colombia)
title_fullStr Callus Type, Growth Regulators, and Phytagel on Indirect Somatic Embryogenesis of Coffee (Coffea arabica L. var. Colombia)
title_full_unstemmed Callus Type, Growth Regulators, and Phytagel on Indirect Somatic Embryogenesis of Coffee (Coffea arabica L. var. Colombia)
title_short Callus Type, Growth Regulators, and Phytagel on Indirect Somatic Embryogenesis of Coffee (Coffea arabica L. var. Colombia)
title_sort callus type, growth regulators, and phytagel on indirect somatic embryogenesis of coffee (coffea arabica l. var. colombia)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10610154/
https://www.ncbi.nlm.nih.gov/pubmed/37896033
http://dx.doi.org/10.3390/plants12203570
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