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Suspension Cell Culture of Polyscias fruticosa (L.) Harms in Bubble-Type Bioreactors—Growth Characteristics, Triterpene Glycosides Accumulation and Biological Activity

Polyscias fruticosa (L.) Harms, or Ming aralia, is a medicinal plant of the Araliaceae family, which is highly valued for its antitoxic, anti-inflammatory, analgesic, antibacterial, anti-asthmatic, adaptogenic, and other properties. The plant can be potentially used to treat diabetes and its complic...

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Autores principales: Titova, Maria V., Kochkin, Dmitry V., Sukhanova, Elena S., Gorshkova, Elena N., Tyurina, Tatiana M., Ivanov, Igor M., Lunkova, Maria K., Tsvetkova, Elena V., Orlova, Anastasia, Popova, Elena V., Nosov, Alexander M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10610180/
https://www.ncbi.nlm.nih.gov/pubmed/37896105
http://dx.doi.org/10.3390/plants12203641
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author Titova, Maria V.
Kochkin, Dmitry V.
Sukhanova, Elena S.
Gorshkova, Elena N.
Tyurina, Tatiana M.
Ivanov, Igor M.
Lunkova, Maria K.
Tsvetkova, Elena V.
Orlova, Anastasia
Popova, Elena V.
Nosov, Alexander M.
author_facet Titova, Maria V.
Kochkin, Dmitry V.
Sukhanova, Elena S.
Gorshkova, Elena N.
Tyurina, Tatiana M.
Ivanov, Igor M.
Lunkova, Maria K.
Tsvetkova, Elena V.
Orlova, Anastasia
Popova, Elena V.
Nosov, Alexander M.
author_sort Titova, Maria V.
collection PubMed
description Polyscias fruticosa (L.) Harms, or Ming aralia, is a medicinal plant of the Araliaceae family, which is highly valued for its antitoxic, anti-inflammatory, analgesic, antibacterial, anti-asthmatic, adaptogenic, and other properties. The plant can be potentially used to treat diabetes and its complications, ischemic brain damage, and Parkinson’s disease. Triterpene glycosides of the oleanane type, such as 3-O-[β-D-glucopyranosyl-(1→4)-β-D-glucuronopyranosyl] oleanolic acid 28-O-β-D-glucopyranosyl ester (PFS), ladyginoside A, and polysciosides A-H, are mainly responsible for biological activities of this species. In this study, cultivation of the cell suspension of P. fruticosa in 20 L bubble-type bioreactors was attempted as a sustainable method for cell biomass production of this valuable species and an alternative to overexploitation of wild plant resources. Cell suspension cultivated in bioreactors under a semi-continuous regime demonstrated satisfactory growth with a specific growth rate of 0.11 day(−1), productivity of 0.32 g (L · day)(−1,) and an economic coefficient of 0.16 but slightly lower maximum biomass accumulation (~6.8 g L(−1)) compared to flask culture (~8.2 g L(−1)). Triterpene glycosides PFS (0.91 mg gDW(−1)) and ladyginoside A (0.77 mg gDW(−1)) were detected in bioreactor-produced cell biomass in higher concentrations compared to cells grown in flasks (0.50 and 0.22 mg gDW(−1), respectively). In antibacterial tests, the minimum inhibitory concentrations (MICs) of cell biomass extracts against the most common pathogens Staphylococcus aureus, methicillin-resistant strain MRSA, Pseudomonas aeruginosa, and Escherichia coli varied within 250–2000 µg mL(−1) which was higher compared to extracts of greenhouse plant leaves (MIC = 4000 µg mL(−1)). Cell biomass extracts also exhibited antioxidant activity, as confirmed by DPPH and TEAC assays. Our results suggest that bioreactor cultivation of P. fruticosa suspension cell culture may be a perspective method for the sustainable biomass production of this species.
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spelling pubmed-106101802023-10-28 Suspension Cell Culture of Polyscias fruticosa (L.) Harms in Bubble-Type Bioreactors—Growth Characteristics, Triterpene Glycosides Accumulation and Biological Activity Titova, Maria V. Kochkin, Dmitry V. Sukhanova, Elena S. Gorshkova, Elena N. Tyurina, Tatiana M. Ivanov, Igor M. Lunkova, Maria K. Tsvetkova, Elena V. Orlova, Anastasia Popova, Elena V. Nosov, Alexander M. Plants (Basel) Article Polyscias fruticosa (L.) Harms, or Ming aralia, is a medicinal plant of the Araliaceae family, which is highly valued for its antitoxic, anti-inflammatory, analgesic, antibacterial, anti-asthmatic, adaptogenic, and other properties. The plant can be potentially used to treat diabetes and its complications, ischemic brain damage, and Parkinson’s disease. Triterpene glycosides of the oleanane type, such as 3-O-[β-D-glucopyranosyl-(1→4)-β-D-glucuronopyranosyl] oleanolic acid 28-O-β-D-glucopyranosyl ester (PFS), ladyginoside A, and polysciosides A-H, are mainly responsible for biological activities of this species. In this study, cultivation of the cell suspension of P. fruticosa in 20 L bubble-type bioreactors was attempted as a sustainable method for cell biomass production of this valuable species and an alternative to overexploitation of wild plant resources. Cell suspension cultivated in bioreactors under a semi-continuous regime demonstrated satisfactory growth with a specific growth rate of 0.11 day(−1), productivity of 0.32 g (L · day)(−1,) and an economic coefficient of 0.16 but slightly lower maximum biomass accumulation (~6.8 g L(−1)) compared to flask culture (~8.2 g L(−1)). Triterpene glycosides PFS (0.91 mg gDW(−1)) and ladyginoside A (0.77 mg gDW(−1)) were detected in bioreactor-produced cell biomass in higher concentrations compared to cells grown in flasks (0.50 and 0.22 mg gDW(−1), respectively). In antibacterial tests, the minimum inhibitory concentrations (MICs) of cell biomass extracts against the most common pathogens Staphylococcus aureus, methicillin-resistant strain MRSA, Pseudomonas aeruginosa, and Escherichia coli varied within 250–2000 µg mL(−1) which was higher compared to extracts of greenhouse plant leaves (MIC = 4000 µg mL(−1)). Cell biomass extracts also exhibited antioxidant activity, as confirmed by DPPH and TEAC assays. Our results suggest that bioreactor cultivation of P. fruticosa suspension cell culture may be a perspective method for the sustainable biomass production of this species. MDPI 2023-10-22 /pmc/articles/PMC10610180/ /pubmed/37896105 http://dx.doi.org/10.3390/plants12203641 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Titova, Maria V.
Kochkin, Dmitry V.
Sukhanova, Elena S.
Gorshkova, Elena N.
Tyurina, Tatiana M.
Ivanov, Igor M.
Lunkova, Maria K.
Tsvetkova, Elena V.
Orlova, Anastasia
Popova, Elena V.
Nosov, Alexander M.
Suspension Cell Culture of Polyscias fruticosa (L.) Harms in Bubble-Type Bioreactors—Growth Characteristics, Triterpene Glycosides Accumulation and Biological Activity
title Suspension Cell Culture of Polyscias fruticosa (L.) Harms in Bubble-Type Bioreactors—Growth Characteristics, Triterpene Glycosides Accumulation and Biological Activity
title_full Suspension Cell Culture of Polyscias fruticosa (L.) Harms in Bubble-Type Bioreactors—Growth Characteristics, Triterpene Glycosides Accumulation and Biological Activity
title_fullStr Suspension Cell Culture of Polyscias fruticosa (L.) Harms in Bubble-Type Bioreactors—Growth Characteristics, Triterpene Glycosides Accumulation and Biological Activity
title_full_unstemmed Suspension Cell Culture of Polyscias fruticosa (L.) Harms in Bubble-Type Bioreactors—Growth Characteristics, Triterpene Glycosides Accumulation and Biological Activity
title_short Suspension Cell Culture of Polyscias fruticosa (L.) Harms in Bubble-Type Bioreactors—Growth Characteristics, Triterpene Glycosides Accumulation and Biological Activity
title_sort suspension cell culture of polyscias fruticosa (l.) harms in bubble-type bioreactors—growth characteristics, triterpene glycosides accumulation and biological activity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10610180/
https://www.ncbi.nlm.nih.gov/pubmed/37896105
http://dx.doi.org/10.3390/plants12203641
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