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Effects of Nanosecond Pulsed Electric Field on Immune Checkpoint Receptors in Melanoma Cells
Checkpoint molecules such as PD-1, LAG-3, and TIM-3 are currently under extensive investigation for their roles in the attenuation of the immune response in cancer. Various methods have been applied to overcome the challenges in this field. This study investigated the effects of nanosecond pulsed el...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10610193/ https://www.ncbi.nlm.nih.gov/pubmed/37895833 http://dx.doi.org/10.3390/ph16101362 |
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author | Sauer, Natalia Szlasa, Wojciech Szewczyk, Anna Novickij, Vitalij Saczko, Jolanta Baczyńska, Dagmara Daczewska, Małgorzata Kulbacka, Julita |
author_facet | Sauer, Natalia Szlasa, Wojciech Szewczyk, Anna Novickij, Vitalij Saczko, Jolanta Baczyńska, Dagmara Daczewska, Małgorzata Kulbacka, Julita |
author_sort | Sauer, Natalia |
collection | PubMed |
description | Checkpoint molecules such as PD-1, LAG-3, and TIM-3 are currently under extensive investigation for their roles in the attenuation of the immune response in cancer. Various methods have been applied to overcome the challenges in this field. This study investigated the effects of nanosecond pulsed electric field (nsPEF) treatment on the expression of immune checkpoint molecules in A375 and C32 melanoma cells. The researchers found that the nsPEF treatment was able to enhance membrane permeabilization and morphological changes in the cell membrane without being cytotoxic. We found that the effects of nsPEFs on melanoma included (1) the transport of vesicles from the inside to the outside of the cells, (2) cell contraction, and (3) the migration of lipids from inside the cells to their peripheries. The treatment increased the expression of PD-1 checkpoint receptors. Furthermore, we also observed potential co-localization or clustering of MHC class II and PD-1 molecules on the cell surface and the secretion of cytokines such as TNF-α and IL-6. These findings suggest that nsPEF treatment could be a viable approach to enhance the delivery of therapeutic agents to cancer cells and to modulate the tumor microenvironment to promote an antitumor immune response. Further studies are needed to explore the mechanisms underlying these effects and their impacts on the antitumor immune response, and to investigate the potential of nsPEF treatment in combination with immune checkpoint inhibitors to improve clinical outcomes for cancer patients. |
format | Online Article Text |
id | pubmed-10610193 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-106101932023-10-28 Effects of Nanosecond Pulsed Electric Field on Immune Checkpoint Receptors in Melanoma Cells Sauer, Natalia Szlasa, Wojciech Szewczyk, Anna Novickij, Vitalij Saczko, Jolanta Baczyńska, Dagmara Daczewska, Małgorzata Kulbacka, Julita Pharmaceuticals (Basel) Article Checkpoint molecules such as PD-1, LAG-3, and TIM-3 are currently under extensive investigation for their roles in the attenuation of the immune response in cancer. Various methods have been applied to overcome the challenges in this field. This study investigated the effects of nanosecond pulsed electric field (nsPEF) treatment on the expression of immune checkpoint molecules in A375 and C32 melanoma cells. The researchers found that the nsPEF treatment was able to enhance membrane permeabilization and morphological changes in the cell membrane without being cytotoxic. We found that the effects of nsPEFs on melanoma included (1) the transport of vesicles from the inside to the outside of the cells, (2) cell contraction, and (3) the migration of lipids from inside the cells to their peripheries. The treatment increased the expression of PD-1 checkpoint receptors. Furthermore, we also observed potential co-localization or clustering of MHC class II and PD-1 molecules on the cell surface and the secretion of cytokines such as TNF-α and IL-6. These findings suggest that nsPEF treatment could be a viable approach to enhance the delivery of therapeutic agents to cancer cells and to modulate the tumor microenvironment to promote an antitumor immune response. Further studies are needed to explore the mechanisms underlying these effects and their impacts on the antitumor immune response, and to investigate the potential of nsPEF treatment in combination with immune checkpoint inhibitors to improve clinical outcomes for cancer patients. MDPI 2023-09-27 /pmc/articles/PMC10610193/ /pubmed/37895833 http://dx.doi.org/10.3390/ph16101362 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Sauer, Natalia Szlasa, Wojciech Szewczyk, Anna Novickij, Vitalij Saczko, Jolanta Baczyńska, Dagmara Daczewska, Małgorzata Kulbacka, Julita Effects of Nanosecond Pulsed Electric Field on Immune Checkpoint Receptors in Melanoma Cells |
title | Effects of Nanosecond Pulsed Electric Field on Immune Checkpoint Receptors in Melanoma Cells |
title_full | Effects of Nanosecond Pulsed Electric Field on Immune Checkpoint Receptors in Melanoma Cells |
title_fullStr | Effects of Nanosecond Pulsed Electric Field on Immune Checkpoint Receptors in Melanoma Cells |
title_full_unstemmed | Effects of Nanosecond Pulsed Electric Field on Immune Checkpoint Receptors in Melanoma Cells |
title_short | Effects of Nanosecond Pulsed Electric Field on Immune Checkpoint Receptors in Melanoma Cells |
title_sort | effects of nanosecond pulsed electric field on immune checkpoint receptors in melanoma cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10610193/ https://www.ncbi.nlm.nih.gov/pubmed/37895833 http://dx.doi.org/10.3390/ph16101362 |
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