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Absence of Coronavirus RNA in Faecal Samples from Wild Primates in Gabon, Central Africa

Coronaviruses (CoVs, Coronaviridae) are a diverse group of viruses that infect mammals, birds, and fish. Seven CoVs infect humans, among which Severe Acute Respiratory Syndrome CoVs-1 and -2 and Middle East respiratory syndrome CoVs have shown how they can impact global health and the economy. Their...

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Autores principales: Mombo, Illich Manfred, Rieu, Océane, Fritz, Matthieu, Boundenga, Larson, Mebaley, Telstar Ndong, Mbou-Boutambe, Clark, Lenguiya, Léadisaelle Hosanna, Maganga, Gael Darren, Rougeron, Virginie, Prugnolle, Franck, Thomas, Fredéric, Leroy, Eric M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10610257/
https://www.ncbi.nlm.nih.gov/pubmed/37887788
http://dx.doi.org/10.3390/pathogens12101272
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author Mombo, Illich Manfred
Rieu, Océane
Fritz, Matthieu
Boundenga, Larson
Mebaley, Telstar Ndong
Mbou-Boutambe, Clark
Lenguiya, Léadisaelle Hosanna
Maganga, Gael Darren
Rougeron, Virginie
Prugnolle, Franck
Thomas, Fredéric
Leroy, Eric M.
author_facet Mombo, Illich Manfred
Rieu, Océane
Fritz, Matthieu
Boundenga, Larson
Mebaley, Telstar Ndong
Mbou-Boutambe, Clark
Lenguiya, Léadisaelle Hosanna
Maganga, Gael Darren
Rougeron, Virginie
Prugnolle, Franck
Thomas, Fredéric
Leroy, Eric M.
author_sort Mombo, Illich Manfred
collection PubMed
description Coronaviruses (CoVs, Coronaviridae) are a diverse group of viruses that infect mammals, birds, and fish. Seven CoVs infect humans, among which Severe Acute Respiratory Syndrome CoVs-1 and -2 and Middle East respiratory syndrome CoVs have shown how they can impact global health and the economy. Their spillover from bats-the natural reservoir-to humans has required intermediary hosts. Prevention requires that active surveillance be conducted on animals. Today, there is no data concerning the genetic diversity of CoVs naturally circulating in wild primates. This study aimed to screen wild great apes and mandrills in Gabon for CoVs. A total of 229 faecal samples of great apes and mandrills collected from 2009 to 2012 in forests and national parks were used for the detection of CoVs by nested PCR using primers targeting a conserved region of the RNA-dependent RNA polymerase. While all samples were negative, this lack of detection could be related to sample size, the transient nature of the infection, or because faecal samples are not suitable for detecting CoVs in primates. A longitudinal study should be performed and other non-invasive methods used to collect respiratory samples to better evaluate the circulation of CoVs in these primates.
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spelling pubmed-106102572023-10-28 Absence of Coronavirus RNA in Faecal Samples from Wild Primates in Gabon, Central Africa Mombo, Illich Manfred Rieu, Océane Fritz, Matthieu Boundenga, Larson Mebaley, Telstar Ndong Mbou-Boutambe, Clark Lenguiya, Léadisaelle Hosanna Maganga, Gael Darren Rougeron, Virginie Prugnolle, Franck Thomas, Fredéric Leroy, Eric M. Pathogens Brief Report Coronaviruses (CoVs, Coronaviridae) are a diverse group of viruses that infect mammals, birds, and fish. Seven CoVs infect humans, among which Severe Acute Respiratory Syndrome CoVs-1 and -2 and Middle East respiratory syndrome CoVs have shown how they can impact global health and the economy. Their spillover from bats-the natural reservoir-to humans has required intermediary hosts. Prevention requires that active surveillance be conducted on animals. Today, there is no data concerning the genetic diversity of CoVs naturally circulating in wild primates. This study aimed to screen wild great apes and mandrills in Gabon for CoVs. A total of 229 faecal samples of great apes and mandrills collected from 2009 to 2012 in forests and national parks were used for the detection of CoVs by nested PCR using primers targeting a conserved region of the RNA-dependent RNA polymerase. While all samples were negative, this lack of detection could be related to sample size, the transient nature of the infection, or because faecal samples are not suitable for detecting CoVs in primates. A longitudinal study should be performed and other non-invasive methods used to collect respiratory samples to better evaluate the circulation of CoVs in these primates. MDPI 2023-10-23 /pmc/articles/PMC10610257/ /pubmed/37887788 http://dx.doi.org/10.3390/pathogens12101272 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Brief Report
Mombo, Illich Manfred
Rieu, Océane
Fritz, Matthieu
Boundenga, Larson
Mebaley, Telstar Ndong
Mbou-Boutambe, Clark
Lenguiya, Léadisaelle Hosanna
Maganga, Gael Darren
Rougeron, Virginie
Prugnolle, Franck
Thomas, Fredéric
Leroy, Eric M.
Absence of Coronavirus RNA in Faecal Samples from Wild Primates in Gabon, Central Africa
title Absence of Coronavirus RNA in Faecal Samples from Wild Primates in Gabon, Central Africa
title_full Absence of Coronavirus RNA in Faecal Samples from Wild Primates in Gabon, Central Africa
title_fullStr Absence of Coronavirus RNA in Faecal Samples from Wild Primates in Gabon, Central Africa
title_full_unstemmed Absence of Coronavirus RNA in Faecal Samples from Wild Primates in Gabon, Central Africa
title_short Absence of Coronavirus RNA in Faecal Samples from Wild Primates in Gabon, Central Africa
title_sort absence of coronavirus rna in faecal samples from wild primates in gabon, central africa
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10610257/
https://www.ncbi.nlm.nih.gov/pubmed/37887788
http://dx.doi.org/10.3390/pathogens12101272
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