Cargando…

Supplementary Effects of Allium hookeri Extract on Glucose Tolerance in Prediabetic Subjects and C57BL/KsJ-db/db Mice

Allium hookeri (AH) has been used as a nutritional and medicinal food in Asia for many years. Our previous studies have described its anti-diabetic, anti-obesity, and anti-inflammatory activities in animal models and prediabetes. This study investigated whether AH could improve glycemia by modulatin...

Descripción completa

Detalles Bibliográficos
Autores principales: Kim, Ji-Su, Kim, Hyun-Ju, Lee, Eun-Byeol, Choi, Ji-Hye, Jung, Jieun, Jang, Hwan-Hee, Park, Shin-Young, Ha, Ki-Chan, Park, Yu-Kyung, Joo, Jong-Cheon, Lee, Sung-Hyen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10610268/
https://www.ncbi.nlm.nih.gov/pubmed/37895834
http://dx.doi.org/10.3390/ph16101364
_version_ 1785128213112946688
author Kim, Ji-Su
Kim, Hyun-Ju
Lee, Eun-Byeol
Choi, Ji-Hye
Jung, Jieun
Jang, Hwan-Hee
Park, Shin-Young
Ha, Ki-Chan
Park, Yu-Kyung
Joo, Jong-Cheon
Lee, Sung-Hyen
author_facet Kim, Ji-Su
Kim, Hyun-Ju
Lee, Eun-Byeol
Choi, Ji-Hye
Jung, Jieun
Jang, Hwan-Hee
Park, Shin-Young
Ha, Ki-Chan
Park, Yu-Kyung
Joo, Jong-Cheon
Lee, Sung-Hyen
author_sort Kim, Ji-Su
collection PubMed
description Allium hookeri (AH) has been used as a nutritional and medicinal food in Asia for many years. Our previous studies have described its anti-diabetic, anti-obesity, and anti-inflammatory activities in animal models and prediabetes. This study investigated whether AH could improve glycemia by modulating insulin secretion in prediabetic subjects through an in-depth study. Eighty prediabetic subjects (100 ≤ fasting plasma glucose < 140 mg/dL) were randomly assigned to a placebo (n = 40) group or an ethanol AH extract (500 mg/day, n = 40) group for 12 weeks. Dietary intake and physical activity, blood glucose (an oral glucose tolerance test for 120 min), insulin (insulin response to oral glucose for 120 min), area under the curve (AUC) of glucose or insulin after oral glucose intake, insulin sensitivity markers, C-peptide, adiponectin, glycated hemoglobin A1c (HbA1c) levels, hematological tests (WBC, RBC, hemoglobin, hematocrit, and platelet count), blood biochemical parameters (ALP, AST, total bilirubin, total protein, albumin, gamma-GT, BUN, creatinine, LD, CK, and hs-CRP), and urine parameters (specific gravity and pH) were examined at both baseline and 12 weeks after supplementation with placebo or AH capsules. Fifty-eight participants (placebo group: 20 men and 10 women; AH group: 13 men and 15 women) completed the study. AH supplementation moderately reduced postprandial blood glucose at 60 min (−6.14 mg/dL, p = 0.061), postprandial insulin levels at 90 min (−16.69 µU/mL, p = 0.017), the glucose AUC at 90 min (−412.52 mg*min/dL, p = 0.021), as well as the insulin AUC at 90 min (−978.77 µU*min/mL, p = 0.021) and 120 min (−1426.41 µU*min/mL, p = 0.015) when compared with the placebo group. However, there were no effects of AH on dietary intake and physical activity; HOMA index; HbAlc; C-peptide; or adiponectin, hematological-, blood biochemical-, and urinary markers. To confirm the effects of AH extract on blood glucose insulin sensitivity, C57BL/6J or C57BL/KsJ-db/db mice were used (n = 8/group). Body weight, fasting plasma glucose level, lipid profiles, liver and renal function, pancreatic histology, and insulin immunoreactivity were assessed. In the diabetic db/db mice, hyperglycemia, which was accompanied by an increase in insulin secretion in diabetic mice, was significantly reduced by AH treatment, resulting in the alleviation of β-cell overcompensation and insulin resistance. We confirmed that AH supplementation can effectively control blood glucose and insulin levels by improving insulin sensitivity and may be a potential agent for glycemic control in subjects with prediabetes and type 2 diabetes mellitus.
format Online
Article
Text
id pubmed-10610268
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-106102682023-10-28 Supplementary Effects of Allium hookeri Extract on Glucose Tolerance in Prediabetic Subjects and C57BL/KsJ-db/db Mice Kim, Ji-Su Kim, Hyun-Ju Lee, Eun-Byeol Choi, Ji-Hye Jung, Jieun Jang, Hwan-Hee Park, Shin-Young Ha, Ki-Chan Park, Yu-Kyung Joo, Jong-Cheon Lee, Sung-Hyen Pharmaceuticals (Basel) Article Allium hookeri (AH) has been used as a nutritional and medicinal food in Asia for many years. Our previous studies have described its anti-diabetic, anti-obesity, and anti-inflammatory activities in animal models and prediabetes. This study investigated whether AH could improve glycemia by modulating insulin secretion in prediabetic subjects through an in-depth study. Eighty prediabetic subjects (100 ≤ fasting plasma glucose < 140 mg/dL) were randomly assigned to a placebo (n = 40) group or an ethanol AH extract (500 mg/day, n = 40) group for 12 weeks. Dietary intake and physical activity, blood glucose (an oral glucose tolerance test for 120 min), insulin (insulin response to oral glucose for 120 min), area under the curve (AUC) of glucose or insulin after oral glucose intake, insulin sensitivity markers, C-peptide, adiponectin, glycated hemoglobin A1c (HbA1c) levels, hematological tests (WBC, RBC, hemoglobin, hematocrit, and platelet count), blood biochemical parameters (ALP, AST, total bilirubin, total protein, albumin, gamma-GT, BUN, creatinine, LD, CK, and hs-CRP), and urine parameters (specific gravity and pH) were examined at both baseline and 12 weeks after supplementation with placebo or AH capsules. Fifty-eight participants (placebo group: 20 men and 10 women; AH group: 13 men and 15 women) completed the study. AH supplementation moderately reduced postprandial blood glucose at 60 min (−6.14 mg/dL, p = 0.061), postprandial insulin levels at 90 min (−16.69 µU/mL, p = 0.017), the glucose AUC at 90 min (−412.52 mg*min/dL, p = 0.021), as well as the insulin AUC at 90 min (−978.77 µU*min/mL, p = 0.021) and 120 min (−1426.41 µU*min/mL, p = 0.015) when compared with the placebo group. However, there were no effects of AH on dietary intake and physical activity; HOMA index; HbAlc; C-peptide; or adiponectin, hematological-, blood biochemical-, and urinary markers. To confirm the effects of AH extract on blood glucose insulin sensitivity, C57BL/6J or C57BL/KsJ-db/db mice were used (n = 8/group). Body weight, fasting plasma glucose level, lipid profiles, liver and renal function, pancreatic histology, and insulin immunoreactivity were assessed. In the diabetic db/db mice, hyperglycemia, which was accompanied by an increase in insulin secretion in diabetic mice, was significantly reduced by AH treatment, resulting in the alleviation of β-cell overcompensation and insulin resistance. We confirmed that AH supplementation can effectively control blood glucose and insulin levels by improving insulin sensitivity and may be a potential agent for glycemic control in subjects with prediabetes and type 2 diabetes mellitus. MDPI 2023-09-27 /pmc/articles/PMC10610268/ /pubmed/37895834 http://dx.doi.org/10.3390/ph16101364 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kim, Ji-Su
Kim, Hyun-Ju
Lee, Eun-Byeol
Choi, Ji-Hye
Jung, Jieun
Jang, Hwan-Hee
Park, Shin-Young
Ha, Ki-Chan
Park, Yu-Kyung
Joo, Jong-Cheon
Lee, Sung-Hyen
Supplementary Effects of Allium hookeri Extract on Glucose Tolerance in Prediabetic Subjects and C57BL/KsJ-db/db Mice
title Supplementary Effects of Allium hookeri Extract on Glucose Tolerance in Prediabetic Subjects and C57BL/KsJ-db/db Mice
title_full Supplementary Effects of Allium hookeri Extract on Glucose Tolerance in Prediabetic Subjects and C57BL/KsJ-db/db Mice
title_fullStr Supplementary Effects of Allium hookeri Extract on Glucose Tolerance in Prediabetic Subjects and C57BL/KsJ-db/db Mice
title_full_unstemmed Supplementary Effects of Allium hookeri Extract on Glucose Tolerance in Prediabetic Subjects and C57BL/KsJ-db/db Mice
title_short Supplementary Effects of Allium hookeri Extract on Glucose Tolerance in Prediabetic Subjects and C57BL/KsJ-db/db Mice
title_sort supplementary effects of allium hookeri extract on glucose tolerance in prediabetic subjects and c57bl/ksj-db/db mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10610268/
https://www.ncbi.nlm.nih.gov/pubmed/37895834
http://dx.doi.org/10.3390/ph16101364
work_keys_str_mv AT kimjisu supplementaryeffectsofalliumhookeriextractonglucosetoleranceinprediabeticsubjectsandc57blksjdbdbmice
AT kimhyunju supplementaryeffectsofalliumhookeriextractonglucosetoleranceinprediabeticsubjectsandc57blksjdbdbmice
AT leeeunbyeol supplementaryeffectsofalliumhookeriextractonglucosetoleranceinprediabeticsubjectsandc57blksjdbdbmice
AT choijihye supplementaryeffectsofalliumhookeriextractonglucosetoleranceinprediabeticsubjectsandc57blksjdbdbmice
AT jungjieun supplementaryeffectsofalliumhookeriextractonglucosetoleranceinprediabeticsubjectsandc57blksjdbdbmice
AT janghwanhee supplementaryeffectsofalliumhookeriextractonglucosetoleranceinprediabeticsubjectsandc57blksjdbdbmice
AT parkshinyoung supplementaryeffectsofalliumhookeriextractonglucosetoleranceinprediabeticsubjectsandc57blksjdbdbmice
AT hakichan supplementaryeffectsofalliumhookeriextractonglucosetoleranceinprediabeticsubjectsandc57blksjdbdbmice
AT parkyukyung supplementaryeffectsofalliumhookeriextractonglucosetoleranceinprediabeticsubjectsandc57blksjdbdbmice
AT joojongcheon supplementaryeffectsofalliumhookeriextractonglucosetoleranceinprediabeticsubjectsandc57blksjdbdbmice
AT leesunghyen supplementaryeffectsofalliumhookeriextractonglucosetoleranceinprediabeticsubjectsandc57blksjdbdbmice