Cargando…

LipL41 and LigA/LigB Gene Silencing on a LipL32 Knockout Leptospira interrogans Reveals the Impact of Multiple Mutations on Virulence

Leptospirosis is a global zoonosis caused by pathogenic bacteria of the genus Leptospira. The application of the CRISPR/Cas9 system has facilitated the generation of mutants and subsequent evaluation of phenotypes. Since DNA breaks induced by RNA-guided Cas9 nuclease are lethal to Leptospira, differ...

Descripción completa

Detalles Bibliográficos
Autores principales:	Fernandes, Luis Guilherme V., Foltran, Bruno B., Teixeira, Aline F., Nascimento, Ana Lucia Tabet Oller
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2023
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10610384/ https://www.ncbi.nlm.nih.gov/pubmed/37887707 http://dx.doi.org/10.3390/pathogens12101191

Ejemplares similares

Evaluation of LipL32 and LigA/LigB Knockdown Mutants in Leptospira interrogans Serovar Copenhageni: Impacts to Proteome and Virulence
por: Fernandes, Luis G. V., et al.
Publicado: (2022)

Evaluation of Leptospira interrogans knockdown mutants for LipL32, LipL41, LipL21, and OmpL1 proteins
por: Fernandes, Luis G. V., et al.
Publicado: (2023)

High yield expression of leptospirosis vaccine candidates LigA and LipL32 in the methylotrophic yeast Pichia pastoris
por: Hartwig, Daiane D, et al.
Publicado: (2010)

Post-translational Modification of LipL32 during Leptospira interrogans Infection
por: Witchell, Timothy D., et al.
Publicado: (2014)

Evolutionary Implication of Outer Membrane Lipoprotein-Encoding Genes ompL1, lipL32 and lipL41 of Pathogenic Leptospira Species
por: Vedhagiri, K., et al.
Publicado: (2009)

An ortholog of the Leptospira interrogans lipoprotein LipL32 aids in the colonization of Pseudoalteromonas tunicata to host surfaces
por: Gardiner, Melissa, et al.
Publicado: (2014)

Expression and purification of LipL32 recombinant protein of Leptospira interrogans in different E.coli strains
por: Bastos, Victor, et al.
Publicado: (2014)

Fine Mapping of the Interaction between C4b-Binding Protein and Outer Membrane Proteins LigA and LigB of Pathogenic Leptospira interrogans
por: Breda, Leandro C. D., et al.
Publicado: (2015)

LipL32 Is a Subsurface Lipoprotein of Leptospira interrogans: Presentation of New Data and Reevaluation of Previous Studies
por: Pinne, Marija, et al.
Publicado: (2013)

Correction: Fine Mapping of the Interaction between C4b-Binding Protein and Outer Membrane Proteins LigA and LigB of Pathogenic Leptospira interrogans
por: Breda, Leandro C. D., et al.
Publicado: (2015)

The leptospiral LipL21 and LipL41 proteins exhibit a broad spectrum of interactions with host cell components
por: MB, Takahashi, et al.
Publicado: (2021)

Sequence analysis of LipL41 and LipL21: Prospective Outer Membrane Proteins (OMPs) in early diagnosing leptospirosis
por: Mosquill, Muzaffar, et al.
Publicado: (2022)

Leptospira interrogans outer membrane protein LipL21 is a potent inhibitor of neutrophil myeloperoxidase
por: Vieira, Monica L., et al.
Publicado: (2018)

Immunoprotective properties of recombinant LigA and LigB in a hamster model of acute leptospirosis
por: Evangelista, Karen V., et al.
Publicado: (2017)

The Terminal Immunoglobulin-Like Repeats of LigA and LigB of Leptospira Enhance Their Binding to Gelatin Binding Domain of Fibronectin and Host Cells
por: Lin, Yi-Pin, et al.
Publicado: (2010)

LipL41, a Hemin Binding Protein from Leptospira santarosai serovar Shermani
por: Lin, Ming-Hsing, et al.
Publicado: (2013)

Characterization of Conserved Combined T and B Cell Epitopes in Leptospira interrogans Major Outer Membrane Proteins OmpL1 and LipL41
por: Lin, Xu'ai, et al.
Publicado: (2011)

Evaluation of real-time PCR targeting the lipL32 gene for diagnosis of Leptospira infection
por: Podgoršek, Daša, et al.
Publicado: (2020)

Heterologous expression of pathogen-specific genes ligA and ligB in the saprophyte Leptospira biflexa confers enhanced adhesion to cultured cells and fibronectin
por: Figueira, Cláudio Pereira, et al.
Publicado: (2011)

LipL21 lipoprotein binding to peptidoglycan enables Leptospira interrogans to escape NOD1 and NOD2 recognition
por: Ratet, Gwenn, et al.
Publicado: (2017)

Cloning and Sequence Analysis of LipL32, a Surface–Exposed Lipoprotein of Pathogenic Leptospira Spp
por: Khodaverdi Darian, Ebrahim, et al.
Publicado: (2013)

Active Components of Leptospira Outer Membrane Protein LipL32 to Toll-Like Receptor 2
por: Hsu, Shen-Hsing, et al.
Publicado: (2017)

A Lateral Flow Assay for the Detection of Leptospira lipL32 Gene Using CRISPR Technology
por: Natarajan, Satheesh, et al.
Publicado: (2023)

Multiple Activities of LigB Potentiate Virulence of Leptospira interrogans: Inhibition of Alternative and Classical Pathways of Complement
por: Choy, Henry A.
Publicado: (2012)

Evaluation of truncated LipL32 expressed by Escherichia coli and Pichia pastoris for serodiagnosis of Leptospira infection in rodents
por: SHIOKAWA, Kanae, et al.
Publicado: (2015)

Development of lipL32 real-time PCR combined with an internal and extraction control for pathogenic Leptospira detection
por: Ahmed, Ahmed A., et al.
Publicado: (2020)

The Multifunctional LigB Adhesin Binds Homeostatic Proteins with Potential Roles in Cutaneous Infection by Pathogenic Leptospira interrogans
por: Choy, Henry A., et al.
Publicado: (2011)

The Effect of Environmental Stresses on lipL32 Gene Expression in Pathogenic Leptospira spp. through Real-Time PCR
por: YASOURI, SONA ROSTAMPOUR, et al.
Publicado: (2020)

Detection of Anti-LipL32 Antibodies in Serum Samples from Horses with Chronic Intraocular Infection with Leptospira spp.
por: Geiger, Tobias, et al.
Publicado: (2021)

Rapid and sensitive point-of-care detection of Leptospira by RPA-CRISPR/Cas12a targeting lipL32
por: Jirawannaporn, Sirawit, et al.
Publicado: (2022)

A LigA Three-Domain Region Protects Hamsters from Lethal Infection by Leptospira interrogans
por: Coutinho, Mariana L., et al.
Publicado: (2011)

RETRACTED ARTICLE: Expression and preliminary characterization of the potential vaccine candidate LipL32 of leptospirosis
por: Govindan, Pothiaraj, et al.
Publicado: (2021)

Oral immunization of rat with chromosomal expression LipL32 in attenuated Salmonella vaccine induces immune respond against pathogenic Leptospira
por: Samakchan, Natthapon, et al.
Publicado: (2021)

The C-terminal variable domain of LigB from Leptospira mediates binding to fibronectin
por: Lin, Yi-Pin, et al.
Publicado: (2008)

Leptospiral outer membrane protein LipL32 induces inflammation and kidney injury in zebrafish larvae
por: Chang, Ming-Yang, et al.
Publicado: (2016)

Identification and Characterization of Lipase Activity and Immunogenicity of LipL from Mycobacterium tuberculosis
por: Cao, Jun, et al.
Publicado: (2015)

Rapid, actionable diagnosis of urban epidemic leptospirosis using a pathogenic Leptospira lipL32-based real-time PCR assay
por: Riediger, Irina N., et al.
Publicado: (2017)

Immune response in hamsters immunised with a recombinant fragment of LigA from Leptospira interrogans, associated with carrier molecules
por: Oliveira, Thaís L, et al.
Publicado: (2016)

Development and evaluation of latex agglutination test coating with recombinant antigen, LipL32 for serodiagnosis of human leptospirosis
por: Thongsukkaeng, Kotchakorn, et al.
Publicado: (2018)

Immunohistochemical detection of Lp25 and LipL32 proteins in skeletal and cardiac muscles of fatal human leptospirosis
por: Iglezias, Silvia D’Andretta, et al.
Publicado: (2020)

Cannot write session to /tmp/vufind_sessions/sess_6tv5ftkqqspe9esfebgg8qv5rm