Cargando…
Influence of the Abiotic Elicitors Ag Salts of Aspartic Acid Derivatives, Self-Organized in Nanofibers with Monomeric and Dimeric Molecular Structures, on the Antioxidant Activity and Stevioside Content in Micropropagated Stevia rebaudiana Bert.
The use of nanomaterials in biotechnology for the in vitro propagation of medical plants and the accumulation of certain biologically active metabolites is becoming an efficient strategy. This study aimed to evaluate the influence of the concentration (0, 1, 10, 50, and 100 mg L(−1)) of two types of...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10610515/ https://www.ncbi.nlm.nih.gov/pubmed/37896037 http://dx.doi.org/10.3390/plants12203574 |
_version_ | 1785128273280237568 |
---|---|
author | Sichanova, Mariana Geneva, Maria Petrova, Maria Miladinova-Georgieva, Kamelia Kirova, Elisaveta Nedev, Trendafil Tsekova, Daniela Ivanova, Viktoria Trendafilova, Antoaneta |
author_facet | Sichanova, Mariana Geneva, Maria Petrova, Maria Miladinova-Georgieva, Kamelia Kirova, Elisaveta Nedev, Trendafil Tsekova, Daniela Ivanova, Viktoria Trendafilova, Antoaneta |
author_sort | Sichanova, Mariana |
collection | PubMed |
description | The use of nanomaterials in biotechnology for the in vitro propagation of medical plants and the accumulation of certain biologically active metabolites is becoming an efficient strategy. This study aimed to evaluate the influence of the concentration (0, 1, 10, 50, and 100 mg L(−1)) of two types of nanofibers on the growth characteristics, the antioxidant status, and the production of steviol glycosides in micropropagated Stevia rebaudiana Bert. plantlets. The nanofibers were synthesized by aspartic acid derivatives (L-Asp) Ag salts self-organized into nanofibers with two different molecular structures: monomeric, containing one residue of L-Asp with one hydrophilic head which bonds one Ag ion (NF1-Ag salt); and dimeric, containing two residues of L-Asp with two hydrophilic heads which bond two Ag ions (NF2-Ag salt). An increase in the shoots from the explants’ number and length, biomass accumulation, and micropropagation rate was achieved in the plants treated with the NF1-Ag salt in concentrations from 1 to 50 mg L(−1) after 30 days of in vitro proliferation compared to the NF2-Ag salt. In contrast, the plants grown on MS media supplemented with NF2-Ag salt exhibited an increase in the level of stevioside, rebaudioside A, and mono- (CQA) and dicaffeoylquinic (DCQA) acids as compared to the NF1-Ag salt. |
format | Online Article Text |
id | pubmed-10610515 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-106105152023-10-28 Influence of the Abiotic Elicitors Ag Salts of Aspartic Acid Derivatives, Self-Organized in Nanofibers with Monomeric and Dimeric Molecular Structures, on the Antioxidant Activity and Stevioside Content in Micropropagated Stevia rebaudiana Bert. Sichanova, Mariana Geneva, Maria Petrova, Maria Miladinova-Georgieva, Kamelia Kirova, Elisaveta Nedev, Trendafil Tsekova, Daniela Ivanova, Viktoria Trendafilova, Antoaneta Plants (Basel) Article The use of nanomaterials in biotechnology for the in vitro propagation of medical plants and the accumulation of certain biologically active metabolites is becoming an efficient strategy. This study aimed to evaluate the influence of the concentration (0, 1, 10, 50, and 100 mg L(−1)) of two types of nanofibers on the growth characteristics, the antioxidant status, and the production of steviol glycosides in micropropagated Stevia rebaudiana Bert. plantlets. The nanofibers were synthesized by aspartic acid derivatives (L-Asp) Ag salts self-organized into nanofibers with two different molecular structures: monomeric, containing one residue of L-Asp with one hydrophilic head which bonds one Ag ion (NF1-Ag salt); and dimeric, containing two residues of L-Asp with two hydrophilic heads which bond two Ag ions (NF2-Ag salt). An increase in the shoots from the explants’ number and length, biomass accumulation, and micropropagation rate was achieved in the plants treated with the NF1-Ag salt in concentrations from 1 to 50 mg L(−1) after 30 days of in vitro proliferation compared to the NF2-Ag salt. In contrast, the plants grown on MS media supplemented with NF2-Ag salt exhibited an increase in the level of stevioside, rebaudioside A, and mono- (CQA) and dicaffeoylquinic (DCQA) acids as compared to the NF1-Ag salt. MDPI 2023-10-14 /pmc/articles/PMC10610515/ /pubmed/37896037 http://dx.doi.org/10.3390/plants12203574 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Sichanova, Mariana Geneva, Maria Petrova, Maria Miladinova-Georgieva, Kamelia Kirova, Elisaveta Nedev, Trendafil Tsekova, Daniela Ivanova, Viktoria Trendafilova, Antoaneta Influence of the Abiotic Elicitors Ag Salts of Aspartic Acid Derivatives, Self-Organized in Nanofibers with Monomeric and Dimeric Molecular Structures, on the Antioxidant Activity and Stevioside Content in Micropropagated Stevia rebaudiana Bert. |
title | Influence of the Abiotic Elicitors Ag Salts of Aspartic Acid Derivatives, Self-Organized in Nanofibers with Monomeric and Dimeric Molecular Structures, on the Antioxidant Activity and Stevioside Content in Micropropagated Stevia rebaudiana Bert. |
title_full | Influence of the Abiotic Elicitors Ag Salts of Aspartic Acid Derivatives, Self-Organized in Nanofibers with Monomeric and Dimeric Molecular Structures, on the Antioxidant Activity and Stevioside Content in Micropropagated Stevia rebaudiana Bert. |
title_fullStr | Influence of the Abiotic Elicitors Ag Salts of Aspartic Acid Derivatives, Self-Organized in Nanofibers with Monomeric and Dimeric Molecular Structures, on the Antioxidant Activity and Stevioside Content in Micropropagated Stevia rebaudiana Bert. |
title_full_unstemmed | Influence of the Abiotic Elicitors Ag Salts of Aspartic Acid Derivatives, Self-Organized in Nanofibers with Monomeric and Dimeric Molecular Structures, on the Antioxidant Activity and Stevioside Content in Micropropagated Stevia rebaudiana Bert. |
title_short | Influence of the Abiotic Elicitors Ag Salts of Aspartic Acid Derivatives, Self-Organized in Nanofibers with Monomeric and Dimeric Molecular Structures, on the Antioxidant Activity and Stevioside Content in Micropropagated Stevia rebaudiana Bert. |
title_sort | influence of the abiotic elicitors ag salts of aspartic acid derivatives, self-organized in nanofibers with monomeric and dimeric molecular structures, on the antioxidant activity and stevioside content in micropropagated stevia rebaudiana bert. |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10610515/ https://www.ncbi.nlm.nih.gov/pubmed/37896037 http://dx.doi.org/10.3390/plants12203574 |
work_keys_str_mv | AT sichanovamariana influenceoftheabioticelicitorsagsaltsofasparticacidderivativesselforganizedinnanofiberswithmonomericanddimericmolecularstructuresontheantioxidantactivityandsteviosidecontentinmicropropagatedsteviarebaudianabert AT genevamaria influenceoftheabioticelicitorsagsaltsofasparticacidderivativesselforganizedinnanofiberswithmonomericanddimericmolecularstructuresontheantioxidantactivityandsteviosidecontentinmicropropagatedsteviarebaudianabert AT petrovamaria influenceoftheabioticelicitorsagsaltsofasparticacidderivativesselforganizedinnanofiberswithmonomericanddimericmolecularstructuresontheantioxidantactivityandsteviosidecontentinmicropropagatedsteviarebaudianabert AT miladinovageorgievakamelia influenceoftheabioticelicitorsagsaltsofasparticacidderivativesselforganizedinnanofiberswithmonomericanddimericmolecularstructuresontheantioxidantactivityandsteviosidecontentinmicropropagatedsteviarebaudianabert AT kirovaelisaveta influenceoftheabioticelicitorsagsaltsofasparticacidderivativesselforganizedinnanofiberswithmonomericanddimericmolecularstructuresontheantioxidantactivityandsteviosidecontentinmicropropagatedsteviarebaudianabert AT nedevtrendafil influenceoftheabioticelicitorsagsaltsofasparticacidderivativesselforganizedinnanofiberswithmonomericanddimericmolecularstructuresontheantioxidantactivityandsteviosidecontentinmicropropagatedsteviarebaudianabert AT tsekovadaniela influenceoftheabioticelicitorsagsaltsofasparticacidderivativesselforganizedinnanofiberswithmonomericanddimericmolecularstructuresontheantioxidantactivityandsteviosidecontentinmicropropagatedsteviarebaudianabert AT ivanovaviktoria influenceoftheabioticelicitorsagsaltsofasparticacidderivativesselforganizedinnanofiberswithmonomericanddimericmolecularstructuresontheantioxidantactivityandsteviosidecontentinmicropropagatedsteviarebaudianabert AT trendafilovaantoaneta influenceoftheabioticelicitorsagsaltsofasparticacidderivativesselforganizedinnanofiberswithmonomericanddimericmolecularstructuresontheantioxidantactivityandsteviosidecontentinmicropropagatedsteviarebaudianabert |