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Establishment of the Maximum Residual Limit in the Milk of Dairy Cows Injected Intramuscularly with Prednisolone

SIMPLE SUMMARY: Prednisolone (PSL) is generally used as an anti-inflammatory and analgesic agent. To establish a withdrawal period using PSL in this study, PSL residual levels and withdrawal time (WT) were assessed in milk after PSL was administered to healthy cows. As a result, the LC-MS/MS method’...

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Autores principales: Rha, Seung Shik, Yang, Ye Jin, Kim, Woo Hyun, Jin, Yeung Bae, Park, Kwang Il, Lee, Hu-Jang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10610623/
https://www.ncbi.nlm.nih.gov/pubmed/37888566
http://dx.doi.org/10.3390/vetsci10100614
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author Rha, Seung Shik
Yang, Ye Jin
Kim, Woo Hyun
Jin, Yeung Bae
Park, Kwang Il
Lee, Hu-Jang
author_facet Rha, Seung Shik
Yang, Ye Jin
Kim, Woo Hyun
Jin, Yeung Bae
Park, Kwang Il
Lee, Hu-Jang
author_sort Rha, Seung Shik
collection PubMed
description SIMPLE SUMMARY: Prednisolone (PSL) is generally used as an anti-inflammatory and analgesic agent. To establish a withdrawal period using PSL in this study, PSL residual levels and withdrawal time (WT) were assessed in milk after PSL was administered to healthy cows. As a result, the LC-MS/MS method’s recovery rate, relative standard deviation, and detection sensitivity met all required standards. At 24 h after treatment, the amount of PSL in milk containing 10 and 20 mg/mL of PSL was lower than the LOD and the LOQ. The time limit for withdrawal was set at 12 h. In summary, since the residual withdrawal period of PSL in milk is shorter than that of the test drug, it suggests that the administration of PSL preparations will produce less milk wastage than that of previous recommendations. ABSTRACT: We measured the levels of prednisolone (PSL) residues in milk of intramuscularly dosed dairy cows and established a withdrawal time (WT) of PSL in milk. Eight healthy Holstein cows were injected with 10 (PSL-1) or 20 (PSL-2) mL of 10 mg/mL of PSL, and then, their milk was sampled at 12 h intervals for five days. PSL residue concentrations in milk were determined using LC-MS/MS. The correlation coefficient of the calibration curve was 0.9976. The limit of detection (LOD) and the limit of quantification (LOQ) were 0.2 µg/kg and 0.6 μg/kg, respectively. Recoveries ranged from 96.5% to 110.0%, and the coefficient of variation was <5.64%. At 24 h after administration, PSL levels in PSL-1 and PSL-2 were below the LOQ in all milk samples. Although this study had a smaller sample size than the European Medicines Agency’s recommendations (n = 20), it was based on the Animal and Plant Quarantine Agency guidelines of the Republic of Korea (n = 8) for the determination of withdrawal periods in milk. We established the withdrawal period for both PSL-1 and PSL-2 in milk at 12 h. In conclusion, we developed an analytical method that is sensitive and can reliably detect PSL in milk, and our estimated WT of PSL in bovine milk is shorter than the current 3-day withdrawal period of PSL in commercial PSL products.
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spelling pubmed-106106232023-10-28 Establishment of the Maximum Residual Limit in the Milk of Dairy Cows Injected Intramuscularly with Prednisolone Rha, Seung Shik Yang, Ye Jin Kim, Woo Hyun Jin, Yeung Bae Park, Kwang Il Lee, Hu-Jang Vet Sci Article SIMPLE SUMMARY: Prednisolone (PSL) is generally used as an anti-inflammatory and analgesic agent. To establish a withdrawal period using PSL in this study, PSL residual levels and withdrawal time (WT) were assessed in milk after PSL was administered to healthy cows. As a result, the LC-MS/MS method’s recovery rate, relative standard deviation, and detection sensitivity met all required standards. At 24 h after treatment, the amount of PSL in milk containing 10 and 20 mg/mL of PSL was lower than the LOD and the LOQ. The time limit for withdrawal was set at 12 h. In summary, since the residual withdrawal period of PSL in milk is shorter than that of the test drug, it suggests that the administration of PSL preparations will produce less milk wastage than that of previous recommendations. ABSTRACT: We measured the levels of prednisolone (PSL) residues in milk of intramuscularly dosed dairy cows and established a withdrawal time (WT) of PSL in milk. Eight healthy Holstein cows were injected with 10 (PSL-1) or 20 (PSL-2) mL of 10 mg/mL of PSL, and then, their milk was sampled at 12 h intervals for five days. PSL residue concentrations in milk were determined using LC-MS/MS. The correlation coefficient of the calibration curve was 0.9976. The limit of detection (LOD) and the limit of quantification (LOQ) were 0.2 µg/kg and 0.6 μg/kg, respectively. Recoveries ranged from 96.5% to 110.0%, and the coefficient of variation was <5.64%. At 24 h after administration, PSL levels in PSL-1 and PSL-2 were below the LOQ in all milk samples. Although this study had a smaller sample size than the European Medicines Agency’s recommendations (n = 20), it was based on the Animal and Plant Quarantine Agency guidelines of the Republic of Korea (n = 8) for the determination of withdrawal periods in milk. We established the withdrawal period for both PSL-1 and PSL-2 in milk at 12 h. In conclusion, we developed an analytical method that is sensitive and can reliably detect PSL in milk, and our estimated WT of PSL in bovine milk is shorter than the current 3-day withdrawal period of PSL in commercial PSL products. MDPI 2023-10-10 /pmc/articles/PMC10610623/ /pubmed/37888566 http://dx.doi.org/10.3390/vetsci10100614 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rha, Seung Shik
Yang, Ye Jin
Kim, Woo Hyun
Jin, Yeung Bae
Park, Kwang Il
Lee, Hu-Jang
Establishment of the Maximum Residual Limit in the Milk of Dairy Cows Injected Intramuscularly with Prednisolone
title Establishment of the Maximum Residual Limit in the Milk of Dairy Cows Injected Intramuscularly with Prednisolone
title_full Establishment of the Maximum Residual Limit in the Milk of Dairy Cows Injected Intramuscularly with Prednisolone
title_fullStr Establishment of the Maximum Residual Limit in the Milk of Dairy Cows Injected Intramuscularly with Prednisolone
title_full_unstemmed Establishment of the Maximum Residual Limit in the Milk of Dairy Cows Injected Intramuscularly with Prednisolone
title_short Establishment of the Maximum Residual Limit in the Milk of Dairy Cows Injected Intramuscularly with Prednisolone
title_sort establishment of the maximum residual limit in the milk of dairy cows injected intramuscularly with prednisolone
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10610623/
https://www.ncbi.nlm.nih.gov/pubmed/37888566
http://dx.doi.org/10.3390/vetsci10100614
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