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Safety and Immunogenicity of Chimeric Pestivirus KD26_E2LOM in Piglets and Calves

A chimeric pestivirus (KD26_E2LOM) was prepared by inserting the E2 gene of the classical swine fever virus (CSFV) LOM strain into the backbone of the bovine viral diarrhea virus (BVDV) KD26 strain. KD26_E2LOM was obtained by transfecting the cDNA pACKD26_E2LOM into PK-15 cells. KD26_E2LOM chimeric...

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Autores principales: Park, Gyu-Nam, Shin, Jihye, Choe, SeEun, Kim, Ki-Sun, Kim, Jae-Jo, Lim, Seong-In, An, Byung-Hyun, Hyun, Bang-Hun, An, Dong-Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10610696/
https://www.ncbi.nlm.nih.gov/pubmed/37897024
http://dx.doi.org/10.3390/vaccines11101622
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author Park, Gyu-Nam
Shin, Jihye
Choe, SeEun
Kim, Ki-Sun
Kim, Jae-Jo
Lim, Seong-In
An, Byung-Hyun
Hyun, Bang-Hun
An, Dong-Jun
author_facet Park, Gyu-Nam
Shin, Jihye
Choe, SeEun
Kim, Ki-Sun
Kim, Jae-Jo
Lim, Seong-In
An, Byung-Hyun
Hyun, Bang-Hun
An, Dong-Jun
author_sort Park, Gyu-Nam
collection PubMed
description A chimeric pestivirus (KD26_E2LOM) was prepared by inserting the E2 gene of the classical swine fever virus (CSFV) LOM strain into the backbone of the bovine viral diarrhea virus (BVDV) KD26 strain. KD26_E2LOM was obtained by transfecting the cDNA pACKD26_E2LOM into PK-15 cells. KD26_E2LOM chimeric pestivirus proliferated to titers of 10(6.5) TCID(50)/mL and 10(8.0) TCID(50)/mL at 96 h post-inoculation into PK-15 cells or MDBK cells, respectively. It also reacted with antibodies specific for CSFV E2 and BVDV E(rns), but not with an anti-BVDV E2 antibody. Piglets (55–60 days old) inoculated with a high dose (10(7.0) TCID(50)/mL) of KD26_E2LOM produced high levels of CSFV E2 antibodies. In addition, no co-habiting pigs were infected with KD26_E2LOM; however, some inoculated pigs excreted the virus, and the virus was detected in some organs. When pregnant sows were inoculated during the first trimester (55–60 days) with a high dose (10(7.0) TCID(50)/mL) of KD26_E2LOM, anti-CSFV E2 antibodies were produced at high levels; chimeric pestivirus was detected in one fetus and in the ileum of one sow. When 5-day-old calves that did not consume colostrum received a high dose (10(7.0) TCID(50)/mL) of KD26_E2LOM, one calf secreted the virus in both feces and nasal fluid on Day 2. A high dose of KD26_E2LOM does not induce specific clinical signs in most animals, does not spread from animal to animal, and generates CSFV E2 antibodies with DVIA functions. Therefore, chimeric pestivirus KD26_E2LOM is a potential CSFV live marker vaccine.
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spelling pubmed-106106962023-10-28 Safety and Immunogenicity of Chimeric Pestivirus KD26_E2LOM in Piglets and Calves Park, Gyu-Nam Shin, Jihye Choe, SeEun Kim, Ki-Sun Kim, Jae-Jo Lim, Seong-In An, Byung-Hyun Hyun, Bang-Hun An, Dong-Jun Vaccines (Basel) Article A chimeric pestivirus (KD26_E2LOM) was prepared by inserting the E2 gene of the classical swine fever virus (CSFV) LOM strain into the backbone of the bovine viral diarrhea virus (BVDV) KD26 strain. KD26_E2LOM was obtained by transfecting the cDNA pACKD26_E2LOM into PK-15 cells. KD26_E2LOM chimeric pestivirus proliferated to titers of 10(6.5) TCID(50)/mL and 10(8.0) TCID(50)/mL at 96 h post-inoculation into PK-15 cells or MDBK cells, respectively. It also reacted with antibodies specific for CSFV E2 and BVDV E(rns), but not with an anti-BVDV E2 antibody. Piglets (55–60 days old) inoculated with a high dose (10(7.0) TCID(50)/mL) of KD26_E2LOM produced high levels of CSFV E2 antibodies. In addition, no co-habiting pigs were infected with KD26_E2LOM; however, some inoculated pigs excreted the virus, and the virus was detected in some organs. When pregnant sows were inoculated during the first trimester (55–60 days) with a high dose (10(7.0) TCID(50)/mL) of KD26_E2LOM, anti-CSFV E2 antibodies were produced at high levels; chimeric pestivirus was detected in one fetus and in the ileum of one sow. When 5-day-old calves that did not consume colostrum received a high dose (10(7.0) TCID(50)/mL) of KD26_E2LOM, one calf secreted the virus in both feces and nasal fluid on Day 2. A high dose of KD26_E2LOM does not induce specific clinical signs in most animals, does not spread from animal to animal, and generates CSFV E2 antibodies with DVIA functions. Therefore, chimeric pestivirus KD26_E2LOM is a potential CSFV live marker vaccine. MDPI 2023-10-21 /pmc/articles/PMC10610696/ /pubmed/37897024 http://dx.doi.org/10.3390/vaccines11101622 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Park, Gyu-Nam
Shin, Jihye
Choe, SeEun
Kim, Ki-Sun
Kim, Jae-Jo
Lim, Seong-In
An, Byung-Hyun
Hyun, Bang-Hun
An, Dong-Jun
Safety and Immunogenicity of Chimeric Pestivirus KD26_E2LOM in Piglets and Calves
title Safety and Immunogenicity of Chimeric Pestivirus KD26_E2LOM in Piglets and Calves
title_full Safety and Immunogenicity of Chimeric Pestivirus KD26_E2LOM in Piglets and Calves
title_fullStr Safety and Immunogenicity of Chimeric Pestivirus KD26_E2LOM in Piglets and Calves
title_full_unstemmed Safety and Immunogenicity of Chimeric Pestivirus KD26_E2LOM in Piglets and Calves
title_short Safety and Immunogenicity of Chimeric Pestivirus KD26_E2LOM in Piglets and Calves
title_sort safety and immunogenicity of chimeric pestivirus kd26_e2lom in piglets and calves
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10610696/
https://www.ncbi.nlm.nih.gov/pubmed/37897024
http://dx.doi.org/10.3390/vaccines11101622
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