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Safety and Immunogenicity of Chimeric Pestivirus KD26_E2LOM in Piglets and Calves
A chimeric pestivirus (KD26_E2LOM) was prepared by inserting the E2 gene of the classical swine fever virus (CSFV) LOM strain into the backbone of the bovine viral diarrhea virus (BVDV) KD26 strain. KD26_E2LOM was obtained by transfecting the cDNA pACKD26_E2LOM into PK-15 cells. KD26_E2LOM chimeric...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10610696/ https://www.ncbi.nlm.nih.gov/pubmed/37897024 http://dx.doi.org/10.3390/vaccines11101622 |
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author | Park, Gyu-Nam Shin, Jihye Choe, SeEun Kim, Ki-Sun Kim, Jae-Jo Lim, Seong-In An, Byung-Hyun Hyun, Bang-Hun An, Dong-Jun |
author_facet | Park, Gyu-Nam Shin, Jihye Choe, SeEun Kim, Ki-Sun Kim, Jae-Jo Lim, Seong-In An, Byung-Hyun Hyun, Bang-Hun An, Dong-Jun |
author_sort | Park, Gyu-Nam |
collection | PubMed |
description | A chimeric pestivirus (KD26_E2LOM) was prepared by inserting the E2 gene of the classical swine fever virus (CSFV) LOM strain into the backbone of the bovine viral diarrhea virus (BVDV) KD26 strain. KD26_E2LOM was obtained by transfecting the cDNA pACKD26_E2LOM into PK-15 cells. KD26_E2LOM chimeric pestivirus proliferated to titers of 10(6.5) TCID(50)/mL and 10(8.0) TCID(50)/mL at 96 h post-inoculation into PK-15 cells or MDBK cells, respectively. It also reacted with antibodies specific for CSFV E2 and BVDV E(rns), but not with an anti-BVDV E2 antibody. Piglets (55–60 days old) inoculated with a high dose (10(7.0) TCID(50)/mL) of KD26_E2LOM produced high levels of CSFV E2 antibodies. In addition, no co-habiting pigs were infected with KD26_E2LOM; however, some inoculated pigs excreted the virus, and the virus was detected in some organs. When pregnant sows were inoculated during the first trimester (55–60 days) with a high dose (10(7.0) TCID(50)/mL) of KD26_E2LOM, anti-CSFV E2 antibodies were produced at high levels; chimeric pestivirus was detected in one fetus and in the ileum of one sow. When 5-day-old calves that did not consume colostrum received a high dose (10(7.0) TCID(50)/mL) of KD26_E2LOM, one calf secreted the virus in both feces and nasal fluid on Day 2. A high dose of KD26_E2LOM does not induce specific clinical signs in most animals, does not spread from animal to animal, and generates CSFV E2 antibodies with DVIA functions. Therefore, chimeric pestivirus KD26_E2LOM is a potential CSFV live marker vaccine. |
format | Online Article Text |
id | pubmed-10610696 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-106106962023-10-28 Safety and Immunogenicity of Chimeric Pestivirus KD26_E2LOM in Piglets and Calves Park, Gyu-Nam Shin, Jihye Choe, SeEun Kim, Ki-Sun Kim, Jae-Jo Lim, Seong-In An, Byung-Hyun Hyun, Bang-Hun An, Dong-Jun Vaccines (Basel) Article A chimeric pestivirus (KD26_E2LOM) was prepared by inserting the E2 gene of the classical swine fever virus (CSFV) LOM strain into the backbone of the bovine viral diarrhea virus (BVDV) KD26 strain. KD26_E2LOM was obtained by transfecting the cDNA pACKD26_E2LOM into PK-15 cells. KD26_E2LOM chimeric pestivirus proliferated to titers of 10(6.5) TCID(50)/mL and 10(8.0) TCID(50)/mL at 96 h post-inoculation into PK-15 cells or MDBK cells, respectively. It also reacted with antibodies specific for CSFV E2 and BVDV E(rns), but not with an anti-BVDV E2 antibody. Piglets (55–60 days old) inoculated with a high dose (10(7.0) TCID(50)/mL) of KD26_E2LOM produced high levels of CSFV E2 antibodies. In addition, no co-habiting pigs were infected with KD26_E2LOM; however, some inoculated pigs excreted the virus, and the virus was detected in some organs. When pregnant sows were inoculated during the first trimester (55–60 days) with a high dose (10(7.0) TCID(50)/mL) of KD26_E2LOM, anti-CSFV E2 antibodies were produced at high levels; chimeric pestivirus was detected in one fetus and in the ileum of one sow. When 5-day-old calves that did not consume colostrum received a high dose (10(7.0) TCID(50)/mL) of KD26_E2LOM, one calf secreted the virus in both feces and nasal fluid on Day 2. A high dose of KD26_E2LOM does not induce specific clinical signs in most animals, does not spread from animal to animal, and generates CSFV E2 antibodies with DVIA functions. Therefore, chimeric pestivirus KD26_E2LOM is a potential CSFV live marker vaccine. MDPI 2023-10-21 /pmc/articles/PMC10610696/ /pubmed/37897024 http://dx.doi.org/10.3390/vaccines11101622 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Park, Gyu-Nam Shin, Jihye Choe, SeEun Kim, Ki-Sun Kim, Jae-Jo Lim, Seong-In An, Byung-Hyun Hyun, Bang-Hun An, Dong-Jun Safety and Immunogenicity of Chimeric Pestivirus KD26_E2LOM in Piglets and Calves |
title | Safety and Immunogenicity of Chimeric Pestivirus KD26_E2LOM in Piglets and Calves |
title_full | Safety and Immunogenicity of Chimeric Pestivirus KD26_E2LOM in Piglets and Calves |
title_fullStr | Safety and Immunogenicity of Chimeric Pestivirus KD26_E2LOM in Piglets and Calves |
title_full_unstemmed | Safety and Immunogenicity of Chimeric Pestivirus KD26_E2LOM in Piglets and Calves |
title_short | Safety and Immunogenicity of Chimeric Pestivirus KD26_E2LOM in Piglets and Calves |
title_sort | safety and immunogenicity of chimeric pestivirus kd26_e2lom in piglets and calves |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10610696/ https://www.ncbi.nlm.nih.gov/pubmed/37897024 http://dx.doi.org/10.3390/vaccines11101622 |
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