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Bothrops atrox and Bothrops lanceolatus Venoms In Vitro Investigation: Composition, Procoagulant Effects, Co-Factor Dependency, and Correction Using Antivenoms
Bothrops venoms are rich in enzymes acting on platelets and coagulation. This action is dependent on two major co-factors, i.e., calcium and phospholipids, while antivenoms variably neutralize venom-related coagulopathy effects. Our aims were (i) to describe the composition of B. atrox and B. lanceo...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10611193/ https://www.ncbi.nlm.nih.gov/pubmed/37888645 http://dx.doi.org/10.3390/toxins15100614 |
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author | Larréché, Sébastien Bousquet, Aurore Chevillard, Lucie Gahoual, Rabah Jourdi, Georges Dupart, Anne-Laure Bachelot-Loza, Christilla Gaussem, Pascale Siguret, Virginie Chippaux, Jean-Philippe Mégarbane, Bruno |
author_facet | Larréché, Sébastien Bousquet, Aurore Chevillard, Lucie Gahoual, Rabah Jourdi, Georges Dupart, Anne-Laure Bachelot-Loza, Christilla Gaussem, Pascale Siguret, Virginie Chippaux, Jean-Philippe Mégarbane, Bruno |
author_sort | Larréché, Sébastien |
collection | PubMed |
description | Bothrops venoms are rich in enzymes acting on platelets and coagulation. This action is dependent on two major co-factors, i.e., calcium and phospholipids, while antivenoms variably neutralize venom-related coagulopathy effects. Our aims were (i) to describe the composition of B. atrox and B. lanceolatus venoms; (ii) to study their activity on the whole blood using rotational thromboelastometry (ROTEM); (iii) to evaluate the contribution of calcium and phospholipids in their activity; and (iv) to compare the effectiveness of four antivenoms (Bothrofav(™), Inoserp(™) South America, Antivipmyn(™) TRI, and PoliVal-ICP(™)) on the procoagulant activity of these two venoms. Venom composition was comparable. Both venoms exhibited hypercoagulant effects. B. lanceolatus venom was completely dependent on calcium but less dependent on phospholipids than B. atrox venom to induce in vitro coagulation. The four antivenoms neutralized the procoagulant activity of the two venoms; however, with quantitative differences. Bothrofav(™) was more effective against both venoms than the three other antivenoms. The relatively similar venom-induced effects in vitro were unexpected considering the opposite clinical manifestations resulting from envenomation (i.e., systemic bleeding with B. atrox and thrombosis with B. lanceolatus). In vivo studies are warranted to better understand the pathophysiology of systemic bleeding and thrombosis associated with Bothrops bites. |
format | Online Article Text |
id | pubmed-10611193 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-106111932023-10-28 Bothrops atrox and Bothrops lanceolatus Venoms In Vitro Investigation: Composition, Procoagulant Effects, Co-Factor Dependency, and Correction Using Antivenoms Larréché, Sébastien Bousquet, Aurore Chevillard, Lucie Gahoual, Rabah Jourdi, Georges Dupart, Anne-Laure Bachelot-Loza, Christilla Gaussem, Pascale Siguret, Virginie Chippaux, Jean-Philippe Mégarbane, Bruno Toxins (Basel) Article Bothrops venoms are rich in enzymes acting on platelets and coagulation. This action is dependent on two major co-factors, i.e., calcium and phospholipids, while antivenoms variably neutralize venom-related coagulopathy effects. Our aims were (i) to describe the composition of B. atrox and B. lanceolatus venoms; (ii) to study their activity on the whole blood using rotational thromboelastometry (ROTEM); (iii) to evaluate the contribution of calcium and phospholipids in their activity; and (iv) to compare the effectiveness of four antivenoms (Bothrofav(™), Inoserp(™) South America, Antivipmyn(™) TRI, and PoliVal-ICP(™)) on the procoagulant activity of these two venoms. Venom composition was comparable. Both venoms exhibited hypercoagulant effects. B. lanceolatus venom was completely dependent on calcium but less dependent on phospholipids than B. atrox venom to induce in vitro coagulation. The four antivenoms neutralized the procoagulant activity of the two venoms; however, with quantitative differences. Bothrofav(™) was more effective against both venoms than the three other antivenoms. The relatively similar venom-induced effects in vitro were unexpected considering the opposite clinical manifestations resulting from envenomation (i.e., systemic bleeding with B. atrox and thrombosis with B. lanceolatus). In vivo studies are warranted to better understand the pathophysiology of systemic bleeding and thrombosis associated with Bothrops bites. MDPI 2023-10-16 /pmc/articles/PMC10611193/ /pubmed/37888645 http://dx.doi.org/10.3390/toxins15100614 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Larréché, Sébastien Bousquet, Aurore Chevillard, Lucie Gahoual, Rabah Jourdi, Georges Dupart, Anne-Laure Bachelot-Loza, Christilla Gaussem, Pascale Siguret, Virginie Chippaux, Jean-Philippe Mégarbane, Bruno Bothrops atrox and Bothrops lanceolatus Venoms In Vitro Investigation: Composition, Procoagulant Effects, Co-Factor Dependency, and Correction Using Antivenoms |
title | Bothrops atrox and Bothrops lanceolatus Venoms In Vitro Investigation: Composition, Procoagulant Effects, Co-Factor Dependency, and Correction Using Antivenoms |
title_full | Bothrops atrox and Bothrops lanceolatus Venoms In Vitro Investigation: Composition, Procoagulant Effects, Co-Factor Dependency, and Correction Using Antivenoms |
title_fullStr | Bothrops atrox and Bothrops lanceolatus Venoms In Vitro Investigation: Composition, Procoagulant Effects, Co-Factor Dependency, and Correction Using Antivenoms |
title_full_unstemmed | Bothrops atrox and Bothrops lanceolatus Venoms In Vitro Investigation: Composition, Procoagulant Effects, Co-Factor Dependency, and Correction Using Antivenoms |
title_short | Bothrops atrox and Bothrops lanceolatus Venoms In Vitro Investigation: Composition, Procoagulant Effects, Co-Factor Dependency, and Correction Using Antivenoms |
title_sort | bothrops atrox and bothrops lanceolatus venoms in vitro investigation: composition, procoagulant effects, co-factor dependency, and correction using antivenoms |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10611193/ https://www.ncbi.nlm.nih.gov/pubmed/37888645 http://dx.doi.org/10.3390/toxins15100614 |
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