Cargando…
Tomato Brown Rugose Fruit Virus: Survival and Disinfection Efficacy on Common Glasshouse Surfaces
Tomato brown rugose fruit virus (ToBRFV) is a contact-transmitted tobamovirus affecting many tomato growing regions of the world. This study investigated the effects of different glasshouse surfaces on the survival of the virus; the efficacy of different disinfectants; and heat treatment against ToB...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10611295/ https://www.ncbi.nlm.nih.gov/pubmed/37896853 http://dx.doi.org/10.3390/v15102076 |
_version_ | 1785128457909305344 |
---|---|
author | Skelton, Anna Frew, Leanne Ward, Richard Hodgson, Rachel Forde, Stephen McDonough, Sam Webster, Gemma Chisnall, Kiera Mynett, Mary Buxton-Kirk, Adam Fowkes, Aimee R. Weekes, Rebecca Fox, Adrian |
author_facet | Skelton, Anna Frew, Leanne Ward, Richard Hodgson, Rachel Forde, Stephen McDonough, Sam Webster, Gemma Chisnall, Kiera Mynett, Mary Buxton-Kirk, Adam Fowkes, Aimee R. Weekes, Rebecca Fox, Adrian |
author_sort | Skelton, Anna |
collection | PubMed |
description | Tomato brown rugose fruit virus (ToBRFV) is a contact-transmitted tobamovirus affecting many tomato growing regions of the world. This study investigated the effects of different glasshouse surfaces on the survival of the virus; the efficacy of different disinfectants; and heat treatment against ToBRFV (surfaces included steel, aluminium, hard plastic, polythene, glass and concrete). A bioassay followed by ELISA was used to check virus viability. ToBRFV survived for at least 7 days on all surfaces tested and on some for at least 6 months. The virus survived for over two hours on hands and gloves. Hand washing was shown to be unreliable for the removal of the virus. Glutaraldehyde and quaternary ammonium compound disinfectants were effective at one hour on all surfaces. Some other disinfectants were effective at one hour of contact time, on all surfaces except concrete. Sodium hypochlorite was partially effective against ToBRFV, even on concrete. A 5 min soak of plastic trays in water at 90 °C was effective at denaturing ToBRFV; however, 5 min at 70 °C was not. Heating infected sap showed the thermal inactivation point to be 90 °C, confirming the hot water treatment results and showing that deactivation was due to the heat treatment and not a washing effect of the water. |
format | Online Article Text |
id | pubmed-10611295 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-106112952023-10-28 Tomato Brown Rugose Fruit Virus: Survival and Disinfection Efficacy on Common Glasshouse Surfaces Skelton, Anna Frew, Leanne Ward, Richard Hodgson, Rachel Forde, Stephen McDonough, Sam Webster, Gemma Chisnall, Kiera Mynett, Mary Buxton-Kirk, Adam Fowkes, Aimee R. Weekes, Rebecca Fox, Adrian Viruses Article Tomato brown rugose fruit virus (ToBRFV) is a contact-transmitted tobamovirus affecting many tomato growing regions of the world. This study investigated the effects of different glasshouse surfaces on the survival of the virus; the efficacy of different disinfectants; and heat treatment against ToBRFV (surfaces included steel, aluminium, hard plastic, polythene, glass and concrete). A bioassay followed by ELISA was used to check virus viability. ToBRFV survived for at least 7 days on all surfaces tested and on some for at least 6 months. The virus survived for over two hours on hands and gloves. Hand washing was shown to be unreliable for the removal of the virus. Glutaraldehyde and quaternary ammonium compound disinfectants were effective at one hour on all surfaces. Some other disinfectants were effective at one hour of contact time, on all surfaces except concrete. Sodium hypochlorite was partially effective against ToBRFV, even on concrete. A 5 min soak of plastic trays in water at 90 °C was effective at denaturing ToBRFV; however, 5 min at 70 °C was not. Heating infected sap showed the thermal inactivation point to be 90 °C, confirming the hot water treatment results and showing that deactivation was due to the heat treatment and not a washing effect of the water. MDPI 2023-10-11 /pmc/articles/PMC10611295/ /pubmed/37896853 http://dx.doi.org/10.3390/v15102076 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Skelton, Anna Frew, Leanne Ward, Richard Hodgson, Rachel Forde, Stephen McDonough, Sam Webster, Gemma Chisnall, Kiera Mynett, Mary Buxton-Kirk, Adam Fowkes, Aimee R. Weekes, Rebecca Fox, Adrian Tomato Brown Rugose Fruit Virus: Survival and Disinfection Efficacy on Common Glasshouse Surfaces |
title | Tomato Brown Rugose Fruit Virus: Survival and Disinfection Efficacy on Common Glasshouse Surfaces |
title_full | Tomato Brown Rugose Fruit Virus: Survival and Disinfection Efficacy on Common Glasshouse Surfaces |
title_fullStr | Tomato Brown Rugose Fruit Virus: Survival and Disinfection Efficacy on Common Glasshouse Surfaces |
title_full_unstemmed | Tomato Brown Rugose Fruit Virus: Survival and Disinfection Efficacy on Common Glasshouse Surfaces |
title_short | Tomato Brown Rugose Fruit Virus: Survival and Disinfection Efficacy on Common Glasshouse Surfaces |
title_sort | tomato brown rugose fruit virus: survival and disinfection efficacy on common glasshouse surfaces |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10611295/ https://www.ncbi.nlm.nih.gov/pubmed/37896853 http://dx.doi.org/10.3390/v15102076 |
work_keys_str_mv | AT skeltonanna tomatobrownrugosefruitvirussurvivalanddisinfectionefficacyoncommonglasshousesurfaces AT frewleanne tomatobrownrugosefruitvirussurvivalanddisinfectionefficacyoncommonglasshousesurfaces AT wardrichard tomatobrownrugosefruitvirussurvivalanddisinfectionefficacyoncommonglasshousesurfaces AT hodgsonrachel tomatobrownrugosefruitvirussurvivalanddisinfectionefficacyoncommonglasshousesurfaces AT fordestephen tomatobrownrugosefruitvirussurvivalanddisinfectionefficacyoncommonglasshousesurfaces AT mcdonoughsam tomatobrownrugosefruitvirussurvivalanddisinfectionefficacyoncommonglasshousesurfaces AT webstergemma tomatobrownrugosefruitvirussurvivalanddisinfectionefficacyoncommonglasshousesurfaces AT chisnallkiera tomatobrownrugosefruitvirussurvivalanddisinfectionefficacyoncommonglasshousesurfaces AT mynettmary tomatobrownrugosefruitvirussurvivalanddisinfectionefficacyoncommonglasshousesurfaces AT buxtonkirkadam tomatobrownrugosefruitvirussurvivalanddisinfectionefficacyoncommonglasshousesurfaces AT fowkesaimeer tomatobrownrugosefruitvirussurvivalanddisinfectionefficacyoncommonglasshousesurfaces AT weekesrebecca tomatobrownrugosefruitvirussurvivalanddisinfectionefficacyoncommonglasshousesurfaces AT foxadrian tomatobrownrugosefruitvirussurvivalanddisinfectionefficacyoncommonglasshousesurfaces |