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First Molecular Identification of Calicophoron daubneyi (Dinnik, 1962) and Paramphistomum leydeni (Nasmark, 1937) in Wild Ruminants from Romania
SIMPLE SUMMARY: Rumen flukes are geographically widespread trematodes affecting wild and domestic ruminants. Severe diarrhoea and weight loss are the major clinical signs, and the disease might be fatal in severely infested individuals. Fifty-two pre-stomachs (rumen and reticulum) obtained from roe...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10611404/ https://www.ncbi.nlm.nih.gov/pubmed/37888555 http://dx.doi.org/10.3390/vetsci10100603 |
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author | Morariu, Sorin Sîrbu, Cătălin Bogdan Tóth, Adrienn Gréta Dărăbuș, Gheorghe Oprescu, Ion Mederle, Narcisa Ilie, Marius Stelian Imre, Mirela Sîrbu, Beatrice Ana-Maria Solymosi, Norbert Florea, Tiana Imre, Kalman |
author_facet | Morariu, Sorin Sîrbu, Cătălin Bogdan Tóth, Adrienn Gréta Dărăbuș, Gheorghe Oprescu, Ion Mederle, Narcisa Ilie, Marius Stelian Imre, Mirela Sîrbu, Beatrice Ana-Maria Solymosi, Norbert Florea, Tiana Imre, Kalman |
author_sort | Morariu, Sorin |
collection | PubMed |
description | SIMPLE SUMMARY: Rumen flukes are geographically widespread trematodes affecting wild and domestic ruminants. Severe diarrhoea and weight loss are the major clinical signs, and the disease might be fatal in severely infested individuals. Fifty-two pre-stomachs (rumen and reticulum) obtained from roe deer (Capreolus capreolus) that were hunted on 14 hunting grounds from Timiș and Arad counties were examined for the presence of paramphistomes. Three (9.09%) out of 33 samples were positive in Timiș County, and one (5.26%) out of 19 samples was positive in Arad County, the main identified species being C. daubneyi and P. leydeni. ABSTRACT: Rumen flukes are geographically widespread trematodes affecting wild and domestic ruminants. The juvenile forms, which are found in the small intestine, are more pathogenic compared to the adults. Severe diarrhoea and weight loss are the major clinical signs, and the disease might be fatal in severely infested individuals. In the last decade, paramphistomosis has been described as an emerging parasitic disease in Europe. This study aimed to identify the rumen fluke species in wild ruminants from western Romania. Fifty-two pre-stomachs obtained from roe deer (Capreolus capreolus) that were hunted on 14 hunting grounds from Timiș and Arad counties were examined for the presence of paramphistomes. Three (9.09%) out of 33 samples were positive in Timiș County, and one (5.26%) out of 19 samples was positive in Arad County. Subsequent PCR testing revealed that three samples were positive for Calicophoron daubneyi and one for Paramphistomum leydeni. The presence of C. daubneyi and P. leydeni in roe deer has not been previously reported in Romania. Two Paramphistomum species—C. daubneyi and P. leydeni—were revealed as the main species of rumen flukes in roe deer from forests in Romania. |
format | Online Article Text |
id | pubmed-10611404 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-106114042023-10-28 First Molecular Identification of Calicophoron daubneyi (Dinnik, 1962) and Paramphistomum leydeni (Nasmark, 1937) in Wild Ruminants from Romania Morariu, Sorin Sîrbu, Cătălin Bogdan Tóth, Adrienn Gréta Dărăbuș, Gheorghe Oprescu, Ion Mederle, Narcisa Ilie, Marius Stelian Imre, Mirela Sîrbu, Beatrice Ana-Maria Solymosi, Norbert Florea, Tiana Imre, Kalman Vet Sci Article SIMPLE SUMMARY: Rumen flukes are geographically widespread trematodes affecting wild and domestic ruminants. Severe diarrhoea and weight loss are the major clinical signs, and the disease might be fatal in severely infested individuals. Fifty-two pre-stomachs (rumen and reticulum) obtained from roe deer (Capreolus capreolus) that were hunted on 14 hunting grounds from Timiș and Arad counties were examined for the presence of paramphistomes. Three (9.09%) out of 33 samples were positive in Timiș County, and one (5.26%) out of 19 samples was positive in Arad County, the main identified species being C. daubneyi and P. leydeni. ABSTRACT: Rumen flukes are geographically widespread trematodes affecting wild and domestic ruminants. The juvenile forms, which are found in the small intestine, are more pathogenic compared to the adults. Severe diarrhoea and weight loss are the major clinical signs, and the disease might be fatal in severely infested individuals. In the last decade, paramphistomosis has been described as an emerging parasitic disease in Europe. This study aimed to identify the rumen fluke species in wild ruminants from western Romania. Fifty-two pre-stomachs obtained from roe deer (Capreolus capreolus) that were hunted on 14 hunting grounds from Timiș and Arad counties were examined for the presence of paramphistomes. Three (9.09%) out of 33 samples were positive in Timiș County, and one (5.26%) out of 19 samples was positive in Arad County. Subsequent PCR testing revealed that three samples were positive for Calicophoron daubneyi and one for Paramphistomum leydeni. The presence of C. daubneyi and P. leydeni in roe deer has not been previously reported in Romania. Two Paramphistomum species—C. daubneyi and P. leydeni—were revealed as the main species of rumen flukes in roe deer from forests in Romania. MDPI 2023-10-03 /pmc/articles/PMC10611404/ /pubmed/37888555 http://dx.doi.org/10.3390/vetsci10100603 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Morariu, Sorin Sîrbu, Cătălin Bogdan Tóth, Adrienn Gréta Dărăbuș, Gheorghe Oprescu, Ion Mederle, Narcisa Ilie, Marius Stelian Imre, Mirela Sîrbu, Beatrice Ana-Maria Solymosi, Norbert Florea, Tiana Imre, Kalman First Molecular Identification of Calicophoron daubneyi (Dinnik, 1962) and Paramphistomum leydeni (Nasmark, 1937) in Wild Ruminants from Romania |
title | First Molecular Identification of Calicophoron daubneyi (Dinnik, 1962) and Paramphistomum leydeni (Nasmark, 1937) in Wild Ruminants from Romania |
title_full | First Molecular Identification of Calicophoron daubneyi (Dinnik, 1962) and Paramphistomum leydeni (Nasmark, 1937) in Wild Ruminants from Romania |
title_fullStr | First Molecular Identification of Calicophoron daubneyi (Dinnik, 1962) and Paramphistomum leydeni (Nasmark, 1937) in Wild Ruminants from Romania |
title_full_unstemmed | First Molecular Identification of Calicophoron daubneyi (Dinnik, 1962) and Paramphistomum leydeni (Nasmark, 1937) in Wild Ruminants from Romania |
title_short | First Molecular Identification of Calicophoron daubneyi (Dinnik, 1962) and Paramphistomum leydeni (Nasmark, 1937) in Wild Ruminants from Romania |
title_sort | first molecular identification of calicophoron daubneyi (dinnik, 1962) and paramphistomum leydeni (nasmark, 1937) in wild ruminants from romania |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10611404/ https://www.ncbi.nlm.nih.gov/pubmed/37888555 http://dx.doi.org/10.3390/vetsci10100603 |
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