Cargando…
Specific Nucleotides in the Common Region of the Begomovirus Tomato Rugose Mosaic Virus (ToRMV) Are Responsible for the Negative Interference over Tomato Severe Rugose Virus (ToSRV) in Mixed Infection
Mixed infection between two or more begomoviruses is commonly found in tomato fields and can affect disease outcomes by increasing symptom severity and viral accumulation compared with single infection. Viruses that affect tomato include tomato severe rugose virus (ToSRV) and tomato rugose mosaic vi...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10611410/ https://www.ncbi.nlm.nih.gov/pubmed/37896851 http://dx.doi.org/10.3390/v15102074 |
_version_ | 1785128484860854272 |
---|---|
author | Nogueira, Angélica M. Barbosa, Tarsiane M. C. Quadros, Ayane F. F. Orílio, Anelise F. Bigão, Marcela C. J. Xavier, César A. D. Ferro, Camila G. Zerbini, Francisco Murilo |
author_facet | Nogueira, Angélica M. Barbosa, Tarsiane M. C. Quadros, Ayane F. F. Orílio, Anelise F. Bigão, Marcela C. J. Xavier, César A. D. Ferro, Camila G. Zerbini, Francisco Murilo |
author_sort | Nogueira, Angélica M. |
collection | PubMed |
description | Mixed infection between two or more begomoviruses is commonly found in tomato fields and can affect disease outcomes by increasing symptom severity and viral accumulation compared with single infection. Viruses that affect tomato include tomato severe rugose virus (ToSRV) and tomato rugose mosaic virus (ToRMV). Previous work showed that in mixed infection, ToRMV negatively affects the infectivity and accumulation of ToSRV. ToSRV and ToRMV share a high degree of sequence identity, including cis-elements in the common region (CR) and their specific recognition sites (iteron-related domain, IRD) within the Rep gene. Here, we investigated if divergent sites in the CR and IRD are involved in the interaction between these two begomoviruses. ToSRV clones were constructed containing the same nucleotides as ToRMV in the CR (ToSRV-A((ToR:CR))), IRD (ToSRV-A((ToR:IRD))) and in both regions (ToSRV-A((ToR:CR+IRD))). When plants were co-inoculated with ToRMV and ToSRV-A((ToR:IRD)), the infectivity and accumulation of ToSRV were negatively affected. In mixed inoculation of ToRMV with ToSRV-A((ToR:CR)), high infectivity of both viruses and high DNA accumulation of ToSRV-A((ToR:CR)) were observed. A decrease in viral accumulation was observed in plants inoculated with ToSRV-A((ToR:CR+IRD)). These results indicate that differences in the CR, but not the IRD, are responsible for the negative interference of ToRMV on ToSRV. |
format | Online Article Text |
id | pubmed-10611410 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-106114102023-10-28 Specific Nucleotides in the Common Region of the Begomovirus Tomato Rugose Mosaic Virus (ToRMV) Are Responsible for the Negative Interference over Tomato Severe Rugose Virus (ToSRV) in Mixed Infection Nogueira, Angélica M. Barbosa, Tarsiane M. C. Quadros, Ayane F. F. Orílio, Anelise F. Bigão, Marcela C. J. Xavier, César A. D. Ferro, Camila G. Zerbini, Francisco Murilo Viruses Article Mixed infection between two or more begomoviruses is commonly found in tomato fields and can affect disease outcomes by increasing symptom severity and viral accumulation compared with single infection. Viruses that affect tomato include tomato severe rugose virus (ToSRV) and tomato rugose mosaic virus (ToRMV). Previous work showed that in mixed infection, ToRMV negatively affects the infectivity and accumulation of ToSRV. ToSRV and ToRMV share a high degree of sequence identity, including cis-elements in the common region (CR) and their specific recognition sites (iteron-related domain, IRD) within the Rep gene. Here, we investigated if divergent sites in the CR and IRD are involved in the interaction between these two begomoviruses. ToSRV clones were constructed containing the same nucleotides as ToRMV in the CR (ToSRV-A((ToR:CR))), IRD (ToSRV-A((ToR:IRD))) and in both regions (ToSRV-A((ToR:CR+IRD))). When plants were co-inoculated with ToRMV and ToSRV-A((ToR:IRD)), the infectivity and accumulation of ToSRV were negatively affected. In mixed inoculation of ToRMV with ToSRV-A((ToR:CR)), high infectivity of both viruses and high DNA accumulation of ToSRV-A((ToR:CR)) were observed. A decrease in viral accumulation was observed in plants inoculated with ToSRV-A((ToR:CR+IRD)). These results indicate that differences in the CR, but not the IRD, are responsible for the negative interference of ToRMV on ToSRV. MDPI 2023-10-11 /pmc/articles/PMC10611410/ /pubmed/37896851 http://dx.doi.org/10.3390/v15102074 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Nogueira, Angélica M. Barbosa, Tarsiane M. C. Quadros, Ayane F. F. Orílio, Anelise F. Bigão, Marcela C. J. Xavier, César A. D. Ferro, Camila G. Zerbini, Francisco Murilo Specific Nucleotides in the Common Region of the Begomovirus Tomato Rugose Mosaic Virus (ToRMV) Are Responsible for the Negative Interference over Tomato Severe Rugose Virus (ToSRV) in Mixed Infection |
title | Specific Nucleotides in the Common Region of the Begomovirus Tomato Rugose Mosaic Virus (ToRMV) Are Responsible for the Negative Interference over Tomato Severe Rugose Virus (ToSRV) in Mixed Infection |
title_full | Specific Nucleotides in the Common Region of the Begomovirus Tomato Rugose Mosaic Virus (ToRMV) Are Responsible for the Negative Interference over Tomato Severe Rugose Virus (ToSRV) in Mixed Infection |
title_fullStr | Specific Nucleotides in the Common Region of the Begomovirus Tomato Rugose Mosaic Virus (ToRMV) Are Responsible for the Negative Interference over Tomato Severe Rugose Virus (ToSRV) in Mixed Infection |
title_full_unstemmed | Specific Nucleotides in the Common Region of the Begomovirus Tomato Rugose Mosaic Virus (ToRMV) Are Responsible for the Negative Interference over Tomato Severe Rugose Virus (ToSRV) in Mixed Infection |
title_short | Specific Nucleotides in the Common Region of the Begomovirus Tomato Rugose Mosaic Virus (ToRMV) Are Responsible for the Negative Interference over Tomato Severe Rugose Virus (ToSRV) in Mixed Infection |
title_sort | specific nucleotides in the common region of the begomovirus tomato rugose mosaic virus (tormv) are responsible for the negative interference over tomato severe rugose virus (tosrv) in mixed infection |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10611410/ https://www.ncbi.nlm.nih.gov/pubmed/37896851 http://dx.doi.org/10.3390/v15102074 |
work_keys_str_mv | AT nogueiraangelicam specificnucleotidesinthecommonregionofthebegomovirustomatorugosemosaicvirustormvareresponsibleforthenegativeinterferenceovertomatosevererugosevirustosrvinmixedinfection AT barbosatarsianemc specificnucleotidesinthecommonregionofthebegomovirustomatorugosemosaicvirustormvareresponsibleforthenegativeinterferenceovertomatosevererugosevirustosrvinmixedinfection AT quadrosayaneff specificnucleotidesinthecommonregionofthebegomovirustomatorugosemosaicvirustormvareresponsibleforthenegativeinterferenceovertomatosevererugosevirustosrvinmixedinfection AT orilioanelisef specificnucleotidesinthecommonregionofthebegomovirustomatorugosemosaicvirustormvareresponsibleforthenegativeinterferenceovertomatosevererugosevirustosrvinmixedinfection AT bigaomarcelacj specificnucleotidesinthecommonregionofthebegomovirustomatorugosemosaicvirustormvareresponsibleforthenegativeinterferenceovertomatosevererugosevirustosrvinmixedinfection AT xaviercesarad specificnucleotidesinthecommonregionofthebegomovirustomatorugosemosaicvirustormvareresponsibleforthenegativeinterferenceovertomatosevererugosevirustosrvinmixedinfection AT ferrocamilag specificnucleotidesinthecommonregionofthebegomovirustomatorugosemosaicvirustormvareresponsibleforthenegativeinterferenceovertomatosevererugosevirustosrvinmixedinfection AT zerbinifranciscomurilo specificnucleotidesinthecommonregionofthebegomovirustomatorugosemosaicvirustormvareresponsibleforthenegativeinterferenceovertomatosevererugosevirustosrvinmixedinfection |