Cargando…
Monocytes and T cells incorporated in full skin equivalents to study innate or adaptive immune reactions after burn injury
INTRODUCTION: Thermal injury often leads to prolonged and excessive inflammation, which hinders the recovery of patients. There is a notable absence of suitable animal-free models for investigating the inflammatory processes following burn injuries, thereby impeding the development of more effective...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10611519/ https://www.ncbi.nlm.nih.gov/pubmed/37901218 http://dx.doi.org/10.3389/fimmu.2023.1264716 |
_version_ | 1785128507117928448 |
---|---|
author | Mulder, Patrick P.G. Vlig, Marcel Elgersma, Anouk Rozemeijer, Lotte Mastenbroek, Leonore S. Middelkoop, Esther Joosten, Irma Koenen, Hans J.P.M. Boekema, Bouke K.H.L. |
author_facet | Mulder, Patrick P.G. Vlig, Marcel Elgersma, Anouk Rozemeijer, Lotte Mastenbroek, Leonore S. Middelkoop, Esther Joosten, Irma Koenen, Hans J.P.M. Boekema, Bouke K.H.L. |
author_sort | Mulder, Patrick P.G. |
collection | PubMed |
description | INTRODUCTION: Thermal injury often leads to prolonged and excessive inflammation, which hinders the recovery of patients. There is a notable absence of suitable animal-free models for investigating the inflammatory processes following burn injuries, thereby impeding the development of more effective therapies to improve burn wound healing in patients. METHODS: In this study, we established a human full skin equivalent (FSE) burn wound model and incorporated human peripheral blood-derived monocytes and T cells. RESULTS: Upon infiltration into the FSEs, the monocytes differentiated into macrophages within a span of 7 days. Burn-injured FSEs exhibited macrophages with increased expression of HLA-DR(+) and elevated production of IL-8 (CXCL8), in comparison to uninjured FSEs. Among the T cells that actively migrated into the FSEs, the majority were CD4(+) and CD25(+). These T cells demonstrated augmented expression of markers associated with regulatory T cell, Th1, or Th17 activity, which coincided with significant heightened cytokine production, including IFN-γ, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-17A, IP-10 (CXCL10), and TGF-β1. Burn injury did not impact the studied effector T cell subsets or cytokine levels. DISCUSSION: Collectively, this study represents a significant advancement in the development of an immunocompetent human skin model, specifically tailored for investigating burn-induced innate or adaptive immune reactions at the site of burn injury. |
format | Online Article Text |
id | pubmed-10611519 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-106115192023-10-28 Monocytes and T cells incorporated in full skin equivalents to study innate or adaptive immune reactions after burn injury Mulder, Patrick P.G. Vlig, Marcel Elgersma, Anouk Rozemeijer, Lotte Mastenbroek, Leonore S. Middelkoop, Esther Joosten, Irma Koenen, Hans J.P.M. Boekema, Bouke K.H.L. Front Immunol Immunology INTRODUCTION: Thermal injury often leads to prolonged and excessive inflammation, which hinders the recovery of patients. There is a notable absence of suitable animal-free models for investigating the inflammatory processes following burn injuries, thereby impeding the development of more effective therapies to improve burn wound healing in patients. METHODS: In this study, we established a human full skin equivalent (FSE) burn wound model and incorporated human peripheral blood-derived monocytes and T cells. RESULTS: Upon infiltration into the FSEs, the monocytes differentiated into macrophages within a span of 7 days. Burn-injured FSEs exhibited macrophages with increased expression of HLA-DR(+) and elevated production of IL-8 (CXCL8), in comparison to uninjured FSEs. Among the T cells that actively migrated into the FSEs, the majority were CD4(+) and CD25(+). These T cells demonstrated augmented expression of markers associated with regulatory T cell, Th1, or Th17 activity, which coincided with significant heightened cytokine production, including IFN-γ, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-17A, IP-10 (CXCL10), and TGF-β1. Burn injury did not impact the studied effector T cell subsets or cytokine levels. DISCUSSION: Collectively, this study represents a significant advancement in the development of an immunocompetent human skin model, specifically tailored for investigating burn-induced innate or adaptive immune reactions at the site of burn injury. Frontiers Media S.A. 2023-10-13 /pmc/articles/PMC10611519/ /pubmed/37901218 http://dx.doi.org/10.3389/fimmu.2023.1264716 Text en Copyright © 2023 Mulder, Vlig, Elgersma, Rozemeijer, Mastenbroek, Middelkoop, Joosten, Koenen and Boekema https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Mulder, Patrick P.G. Vlig, Marcel Elgersma, Anouk Rozemeijer, Lotte Mastenbroek, Leonore S. Middelkoop, Esther Joosten, Irma Koenen, Hans J.P.M. Boekema, Bouke K.H.L. Monocytes and T cells incorporated in full skin equivalents to study innate or adaptive immune reactions after burn injury |
title | Monocytes and T cells incorporated in full skin equivalents to study innate or adaptive immune reactions after burn injury |
title_full | Monocytes and T cells incorporated in full skin equivalents to study innate or adaptive immune reactions after burn injury |
title_fullStr | Monocytes and T cells incorporated in full skin equivalents to study innate or adaptive immune reactions after burn injury |
title_full_unstemmed | Monocytes and T cells incorporated in full skin equivalents to study innate or adaptive immune reactions after burn injury |
title_short | Monocytes and T cells incorporated in full skin equivalents to study innate or adaptive immune reactions after burn injury |
title_sort | monocytes and t cells incorporated in full skin equivalents to study innate or adaptive immune reactions after burn injury |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10611519/ https://www.ncbi.nlm.nih.gov/pubmed/37901218 http://dx.doi.org/10.3389/fimmu.2023.1264716 |
work_keys_str_mv | AT mulderpatrickpg monocytesandtcellsincorporatedinfullskinequivalentstostudyinnateoradaptiveimmunereactionsafterburninjury AT vligmarcel monocytesandtcellsincorporatedinfullskinequivalentstostudyinnateoradaptiveimmunereactionsafterburninjury AT elgersmaanouk monocytesandtcellsincorporatedinfullskinequivalentstostudyinnateoradaptiveimmunereactionsafterburninjury AT rozemeijerlotte monocytesandtcellsincorporatedinfullskinequivalentstostudyinnateoradaptiveimmunereactionsafterburninjury AT mastenbroekleonores monocytesandtcellsincorporatedinfullskinequivalentstostudyinnateoradaptiveimmunereactionsafterburninjury AT middelkoopesther monocytesandtcellsincorporatedinfullskinequivalentstostudyinnateoradaptiveimmunereactionsafterburninjury AT joostenirma monocytesandtcellsincorporatedinfullskinequivalentstostudyinnateoradaptiveimmunereactionsafterburninjury AT koenenhansjpm monocytesandtcellsincorporatedinfullskinequivalentstostudyinnateoradaptiveimmunereactionsafterburninjury AT boekemaboukekhl monocytesandtcellsincorporatedinfullskinequivalentstostudyinnateoradaptiveimmunereactionsafterburninjury |