Cargando…

Study on Carbohydrate Metabolism in Adult Zebrafish (Danio rerio)

Excessive carbohydrate intake leads to metabolic disorders in fish. However, few literatures have reported the appropriate carbohydrate level for zebrafish, and the metabolic response to dietary carbohydrate remains largely unknown in zebrafish. This study assessed the responses of zebrafish and zeb...

Descripción completa

Detalles Bibliográficos
Autores principales: Xi, Longwei, Lu, Qisheng, Liu, Yulong, Gong, Yulong, Liu, Haokun, Jin, Junyan, Zhang, Zhimin, Yang, Yunxia, Zhu, Xiaoming, Han, Dong, Xie, Shouqi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10611541/
https://www.ncbi.nlm.nih.gov/pubmed/37901279
http://dx.doi.org/10.1155/2023/1397508
_version_ 1785128512045187072
author Xi, Longwei
Lu, Qisheng
Liu, Yulong
Gong, Yulong
Liu, Haokun
Jin, Junyan
Zhang, Zhimin
Yang, Yunxia
Zhu, Xiaoming
Han, Dong
Xie, Shouqi
author_facet Xi, Longwei
Lu, Qisheng
Liu, Yulong
Gong, Yulong
Liu, Haokun
Jin, Junyan
Zhang, Zhimin
Yang, Yunxia
Zhu, Xiaoming
Han, Dong
Xie, Shouqi
author_sort Xi, Longwei
collection PubMed
description Excessive carbohydrate intake leads to metabolic disorders in fish. However, few literatures have reported the appropriate carbohydrate level for zebrafish, and the metabolic response to dietary carbohydrate remains largely unknown in zebrafish. This study assessed the responses of zebrafish and zebrafish liver cell line (ZFL) to different carbohydrate levels. In vivo results showed that ≥30% dietary dextrin levels significantly increased the plasma glucose content, activated the expression of hepatic glycolysis-related genes, and inhibited the expression of hepatic gluconeogenesis-related genes in zebrafish. Oil red O staining, triglyceride content, and Hematoxylin-Eosin staining results showed that dietary dextrin levels of ≥30% significantly increased lipid accumulation and liver damage, as well as processes related to glycolipid metabolism and inflammation in zebrafish. In ZFL, the transcription factor sterol regulatory element binding protein-1c signal intensity, 4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene (BODIPY 493/503) signal intensity, and triglyceride content were also significantly increased when incubated in high glucose, along with abnormal glycolipid metabolism and increased inflammation-related genes. In conclusion, we demonstrated that the maximum dietary carbohydrate level in adult zebrafish should be less than 30%. Excess dietary carbohydrates (30%–50%) caused hepatic steatosis and damage to zebrafish, similar to that seen in aquaculture species. Thus, this study assessed responses to different carbohydrate levels in zebrafish and illustrated that zebrafish is an optimal model for investigating glucose metabolism in some aquatic animals.
format Online
Article
Text
id pubmed-10611541
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-106115412023-10-28 Study on Carbohydrate Metabolism in Adult Zebrafish (Danio rerio) Xi, Longwei Lu, Qisheng Liu, Yulong Gong, Yulong Liu, Haokun Jin, Junyan Zhang, Zhimin Yang, Yunxia Zhu, Xiaoming Han, Dong Xie, Shouqi Aquac Nutr Research Article Excessive carbohydrate intake leads to metabolic disorders in fish. However, few literatures have reported the appropriate carbohydrate level for zebrafish, and the metabolic response to dietary carbohydrate remains largely unknown in zebrafish. This study assessed the responses of zebrafish and zebrafish liver cell line (ZFL) to different carbohydrate levels. In vivo results showed that ≥30% dietary dextrin levels significantly increased the plasma glucose content, activated the expression of hepatic glycolysis-related genes, and inhibited the expression of hepatic gluconeogenesis-related genes in zebrafish. Oil red O staining, triglyceride content, and Hematoxylin-Eosin staining results showed that dietary dextrin levels of ≥30% significantly increased lipid accumulation and liver damage, as well as processes related to glycolipid metabolism and inflammation in zebrafish. In ZFL, the transcription factor sterol regulatory element binding protein-1c signal intensity, 4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene (BODIPY 493/503) signal intensity, and triglyceride content were also significantly increased when incubated in high glucose, along with abnormal glycolipid metabolism and increased inflammation-related genes. In conclusion, we demonstrated that the maximum dietary carbohydrate level in adult zebrafish should be less than 30%. Excess dietary carbohydrates (30%–50%) caused hepatic steatosis and damage to zebrafish, similar to that seen in aquaculture species. Thus, this study assessed responses to different carbohydrate levels in zebrafish and illustrated that zebrafish is an optimal model for investigating glucose metabolism in some aquatic animals. Hindawi 2023-10-20 /pmc/articles/PMC10611541/ /pubmed/37901279 http://dx.doi.org/10.1155/2023/1397508 Text en Copyright © 2023 Longwei Xi et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Xi, Longwei
Lu, Qisheng
Liu, Yulong
Gong, Yulong
Liu, Haokun
Jin, Junyan
Zhang, Zhimin
Yang, Yunxia
Zhu, Xiaoming
Han, Dong
Xie, Shouqi
Study on Carbohydrate Metabolism in Adult Zebrafish (Danio rerio)
title Study on Carbohydrate Metabolism in Adult Zebrafish (Danio rerio)
title_full Study on Carbohydrate Metabolism in Adult Zebrafish (Danio rerio)
title_fullStr Study on Carbohydrate Metabolism in Adult Zebrafish (Danio rerio)
title_full_unstemmed Study on Carbohydrate Metabolism in Adult Zebrafish (Danio rerio)
title_short Study on Carbohydrate Metabolism in Adult Zebrafish (Danio rerio)
title_sort study on carbohydrate metabolism in adult zebrafish (danio rerio)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10611541/
https://www.ncbi.nlm.nih.gov/pubmed/37901279
http://dx.doi.org/10.1155/2023/1397508
work_keys_str_mv AT xilongwei studyoncarbohydratemetabolisminadultzebrafishdaniorerio
AT luqisheng studyoncarbohydratemetabolisminadultzebrafishdaniorerio
AT liuyulong studyoncarbohydratemetabolisminadultzebrafishdaniorerio
AT gongyulong studyoncarbohydratemetabolisminadultzebrafishdaniorerio
AT liuhaokun studyoncarbohydratemetabolisminadultzebrafishdaniorerio
AT jinjunyan studyoncarbohydratemetabolisminadultzebrafishdaniorerio
AT zhangzhimin studyoncarbohydratemetabolisminadultzebrafishdaniorerio
AT yangyunxia studyoncarbohydratemetabolisminadultzebrafishdaniorerio
AT zhuxiaoming studyoncarbohydratemetabolisminadultzebrafishdaniorerio
AT handong studyoncarbohydratemetabolisminadultzebrafishdaniorerio
AT xieshouqi studyoncarbohydratemetabolisminadultzebrafishdaniorerio