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Study on Carbohydrate Metabolism in Adult Zebrafish (Danio rerio)
Excessive carbohydrate intake leads to metabolic disorders in fish. However, few literatures have reported the appropriate carbohydrate level for zebrafish, and the metabolic response to dietary carbohydrate remains largely unknown in zebrafish. This study assessed the responses of zebrafish and zeb...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10611541/ https://www.ncbi.nlm.nih.gov/pubmed/37901279 http://dx.doi.org/10.1155/2023/1397508 |
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author | Xi, Longwei Lu, Qisheng Liu, Yulong Gong, Yulong Liu, Haokun Jin, Junyan Zhang, Zhimin Yang, Yunxia Zhu, Xiaoming Han, Dong Xie, Shouqi |
author_facet | Xi, Longwei Lu, Qisheng Liu, Yulong Gong, Yulong Liu, Haokun Jin, Junyan Zhang, Zhimin Yang, Yunxia Zhu, Xiaoming Han, Dong Xie, Shouqi |
author_sort | Xi, Longwei |
collection | PubMed |
description | Excessive carbohydrate intake leads to metabolic disorders in fish. However, few literatures have reported the appropriate carbohydrate level for zebrafish, and the metabolic response to dietary carbohydrate remains largely unknown in zebrafish. This study assessed the responses of zebrafish and zebrafish liver cell line (ZFL) to different carbohydrate levels. In vivo results showed that ≥30% dietary dextrin levels significantly increased the plasma glucose content, activated the expression of hepatic glycolysis-related genes, and inhibited the expression of hepatic gluconeogenesis-related genes in zebrafish. Oil red O staining, triglyceride content, and Hematoxylin-Eosin staining results showed that dietary dextrin levels of ≥30% significantly increased lipid accumulation and liver damage, as well as processes related to glycolipid metabolism and inflammation in zebrafish. In ZFL, the transcription factor sterol regulatory element binding protein-1c signal intensity, 4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene (BODIPY 493/503) signal intensity, and triglyceride content were also significantly increased when incubated in high glucose, along with abnormal glycolipid metabolism and increased inflammation-related genes. In conclusion, we demonstrated that the maximum dietary carbohydrate level in adult zebrafish should be less than 30%. Excess dietary carbohydrates (30%–50%) caused hepatic steatosis and damage to zebrafish, similar to that seen in aquaculture species. Thus, this study assessed responses to different carbohydrate levels in zebrafish and illustrated that zebrafish is an optimal model for investigating glucose metabolism in some aquatic animals. |
format | Online Article Text |
id | pubmed-10611541 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-106115412023-10-28 Study on Carbohydrate Metabolism in Adult Zebrafish (Danio rerio) Xi, Longwei Lu, Qisheng Liu, Yulong Gong, Yulong Liu, Haokun Jin, Junyan Zhang, Zhimin Yang, Yunxia Zhu, Xiaoming Han, Dong Xie, Shouqi Aquac Nutr Research Article Excessive carbohydrate intake leads to metabolic disorders in fish. However, few literatures have reported the appropriate carbohydrate level for zebrafish, and the metabolic response to dietary carbohydrate remains largely unknown in zebrafish. This study assessed the responses of zebrafish and zebrafish liver cell line (ZFL) to different carbohydrate levels. In vivo results showed that ≥30% dietary dextrin levels significantly increased the plasma glucose content, activated the expression of hepatic glycolysis-related genes, and inhibited the expression of hepatic gluconeogenesis-related genes in zebrafish. Oil red O staining, triglyceride content, and Hematoxylin-Eosin staining results showed that dietary dextrin levels of ≥30% significantly increased lipid accumulation and liver damage, as well as processes related to glycolipid metabolism and inflammation in zebrafish. In ZFL, the transcription factor sterol regulatory element binding protein-1c signal intensity, 4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene (BODIPY 493/503) signal intensity, and triglyceride content were also significantly increased when incubated in high glucose, along with abnormal glycolipid metabolism and increased inflammation-related genes. In conclusion, we demonstrated that the maximum dietary carbohydrate level in adult zebrafish should be less than 30%. Excess dietary carbohydrates (30%–50%) caused hepatic steatosis and damage to zebrafish, similar to that seen in aquaculture species. Thus, this study assessed responses to different carbohydrate levels in zebrafish and illustrated that zebrafish is an optimal model for investigating glucose metabolism in some aquatic animals. Hindawi 2023-10-20 /pmc/articles/PMC10611541/ /pubmed/37901279 http://dx.doi.org/10.1155/2023/1397508 Text en Copyright © 2023 Longwei Xi et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Xi, Longwei Lu, Qisheng Liu, Yulong Gong, Yulong Liu, Haokun Jin, Junyan Zhang, Zhimin Yang, Yunxia Zhu, Xiaoming Han, Dong Xie, Shouqi Study on Carbohydrate Metabolism in Adult Zebrafish (Danio rerio) |
title | Study on Carbohydrate Metabolism in Adult Zebrafish (Danio rerio) |
title_full | Study on Carbohydrate Metabolism in Adult Zebrafish (Danio rerio) |
title_fullStr | Study on Carbohydrate Metabolism in Adult Zebrafish (Danio rerio) |
title_full_unstemmed | Study on Carbohydrate Metabolism in Adult Zebrafish (Danio rerio) |
title_short | Study on Carbohydrate Metabolism in Adult Zebrafish (Danio rerio) |
title_sort | study on carbohydrate metabolism in adult zebrafish (danio rerio) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10611541/ https://www.ncbi.nlm.nih.gov/pubmed/37901279 http://dx.doi.org/10.1155/2023/1397508 |
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