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PbrChiA: a key chitinase of pear in response to Botryosphaeria dothidea infection by interacting with PbrLYK1b2 and down-regulating ROS accumulation

Pear ring rot, caused by the pathogenic fungi Botryosphaeria dothidea, seriously affects pear production. While the infection-induced reactive oxygen species (ROS) burst of infected plants limits the proliferation of B. dothidea during the early infection stage, high ROS levels can also contribute t...

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Autores principales: Chen, Qiming, Dong, Huizhen, Li, Qionghou, Sun, Xun, Qiao, Xin, Yin, Hao, Xie, Zhihua, Qi, Kaijie, Huang, Xiaosan, Zhang, Shaoling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10611555/
https://www.ncbi.nlm.nih.gov/pubmed/37899950
http://dx.doi.org/10.1093/hr/uhad188
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author Chen, Qiming
Dong, Huizhen
Li, Qionghou
Sun, Xun
Qiao, Xin
Yin, Hao
Xie, Zhihua
Qi, Kaijie
Huang, Xiaosan
Zhang, Shaoling
author_facet Chen, Qiming
Dong, Huizhen
Li, Qionghou
Sun, Xun
Qiao, Xin
Yin, Hao
Xie, Zhihua
Qi, Kaijie
Huang, Xiaosan
Zhang, Shaoling
author_sort Chen, Qiming
collection PubMed
description Pear ring rot, caused by the pathogenic fungi Botryosphaeria dothidea, seriously affects pear production. While the infection-induced reactive oxygen species (ROS) burst of infected plants limits the proliferation of B. dothidea during the early infection stage, high ROS levels can also contribute to their growth during the later necrotrophic infection stage. Therefore, it is important to understand how plants balance ROS levels and resistance to pathogenic B. dothidea during the later stage. In this study, we identified PbrChiA, a glycosyl hydrolases 18 (GH18) chitinase-encoding gene with high infection-induced expression, through a comparative transcriptome analysis. Artificial substitution, stable overexpression, and virus induced gene silencing (VIGS) experiments demonstrated that PbrChiA can positively regulate pear resistance as a secreted chitinase to break down B. dothidea mycelium in vitro and that overexpression of PbrChiA suppressed infection-induced ROS accumulation. Further analysis revealed that PbrChiA can bind to the ectodomain of PbrLYK1b2, and this interaction suppressed PbrLYK1b2-mediated chitin-induced ROS accumulation. Collectively, we propose that the combination of higher antifungal activity from abundant PbrChiA and lower ROS levels during later necrotrophic infection stage confer resistance of pear against B. dothidea.
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spelling pubmed-106115552023-10-29 PbrChiA: a key chitinase of pear in response to Botryosphaeria dothidea infection by interacting with PbrLYK1b2 and down-regulating ROS accumulation Chen, Qiming Dong, Huizhen Li, Qionghou Sun, Xun Qiao, Xin Yin, Hao Xie, Zhihua Qi, Kaijie Huang, Xiaosan Zhang, Shaoling Hortic Res Article Pear ring rot, caused by the pathogenic fungi Botryosphaeria dothidea, seriously affects pear production. While the infection-induced reactive oxygen species (ROS) burst of infected plants limits the proliferation of B. dothidea during the early infection stage, high ROS levels can also contribute to their growth during the later necrotrophic infection stage. Therefore, it is important to understand how plants balance ROS levels and resistance to pathogenic B. dothidea during the later stage. In this study, we identified PbrChiA, a glycosyl hydrolases 18 (GH18) chitinase-encoding gene with high infection-induced expression, through a comparative transcriptome analysis. Artificial substitution, stable overexpression, and virus induced gene silencing (VIGS) experiments demonstrated that PbrChiA can positively regulate pear resistance as a secreted chitinase to break down B. dothidea mycelium in vitro and that overexpression of PbrChiA suppressed infection-induced ROS accumulation. Further analysis revealed that PbrChiA can bind to the ectodomain of PbrLYK1b2, and this interaction suppressed PbrLYK1b2-mediated chitin-induced ROS accumulation. Collectively, we propose that the combination of higher antifungal activity from abundant PbrChiA and lower ROS levels during later necrotrophic infection stage confer resistance of pear against B. dothidea. Oxford University Press 2023-09-19 /pmc/articles/PMC10611555/ /pubmed/37899950 http://dx.doi.org/10.1093/hr/uhad188 Text en © The Author(s) 2023. Published by Oxford University Press on behalf of Nanjing Agricultural University. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
Chen, Qiming
Dong, Huizhen
Li, Qionghou
Sun, Xun
Qiao, Xin
Yin, Hao
Xie, Zhihua
Qi, Kaijie
Huang, Xiaosan
Zhang, Shaoling
PbrChiA: a key chitinase of pear in response to Botryosphaeria dothidea infection by interacting with PbrLYK1b2 and down-regulating ROS accumulation
title PbrChiA: a key chitinase of pear in response to Botryosphaeria dothidea infection by interacting with PbrLYK1b2 and down-regulating ROS accumulation
title_full PbrChiA: a key chitinase of pear in response to Botryosphaeria dothidea infection by interacting with PbrLYK1b2 and down-regulating ROS accumulation
title_fullStr PbrChiA: a key chitinase of pear in response to Botryosphaeria dothidea infection by interacting with PbrLYK1b2 and down-regulating ROS accumulation
title_full_unstemmed PbrChiA: a key chitinase of pear in response to Botryosphaeria dothidea infection by interacting with PbrLYK1b2 and down-regulating ROS accumulation
title_short PbrChiA: a key chitinase of pear in response to Botryosphaeria dothidea infection by interacting with PbrLYK1b2 and down-regulating ROS accumulation
title_sort pbrchia: a key chitinase of pear in response to botryosphaeria dothidea infection by interacting with pbrlyk1b2 and down-regulating ros accumulation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10611555/
https://www.ncbi.nlm.nih.gov/pubmed/37899950
http://dx.doi.org/10.1093/hr/uhad188
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