Enhancing Precision and Efficiency of Cas9-Mediated Knockin Through Combinatorial Fusions of DNA Repair Proteins

Cas9 targets genomic loci with high specificity. For knockin with double-strand break repair, however, Cas9 often leads to unintended on-target knockout rather than intended edits. This imprecision is a barrier for direct in vivo editing where clonal selection is not feasible. In this study, we demo...

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Detalles Bibliográficos
Autores principales: Richardson, Ryan R., Steyert, Marilyn, Khim, Saovleak N., Crutcher, Garrett W., Brandenburg, Cheryl, Robertson, Colin D., Romanowski, Andrea J., Inen, Jeffrey, Altas, Bekir, Poulopoulos, Alexandros
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mary Ann Liebert, Inc., publishers 2023
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10611978/
https://www.ncbi.nlm.nih.gov/pubmed/37713292
http://dx.doi.org/10.1089/crispr.2023.0036

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