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EBV Impact in Peripheral Macrophages’ Polarization Cytokines in Pediatric Patients
Macrophages are exceptionally flexible cells. The presence of inflammatory cytokines such as IFN-γ and TNF-α results in an M1 (CD68) activation, while cytokines such as IL-10 or TGF-β induce the M2 (CD163) activation. Our aim was to study the behavior of peripheral cytokines involved in macrophage p...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10612087/ https://www.ncbi.nlm.nih.gov/pubmed/37896882 http://dx.doi.org/10.3390/v15102105 |
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author | Moyano, Agustina Ferressini Gerpe, Natalia Amarillo, Maria Eugenia De Matteo, Elena Preciado, Maria Victoria Caldirola, Maria Soledad Chabay, Paola |
author_facet | Moyano, Agustina Ferressini Gerpe, Natalia Amarillo, Maria Eugenia De Matteo, Elena Preciado, Maria Victoria Caldirola, Maria Soledad Chabay, Paola |
author_sort | Moyano, Agustina |
collection | PubMed |
description | Macrophages are exceptionally flexible cells. The presence of inflammatory cytokines such as IFN-γ and TNF-α results in an M1 (CD68) activation, while cytokines such as IL-10 or TGF-β induce the M2 (CD163) activation. Our aim was to study the behavior of peripheral cytokines involved in macrophage polarization and relate them with tissue findings to further comprehend the role of macrophages in EBV pediatric infection. We studied cytokine expression in tonsils and peripheral blood samples of children in different stages of infection. Peripheral cytokines were compared with macrophage polarization markers and viral protein expression in tonsils. Only IL-10 showed a negative correlation between compartments, exclusively in patients undergoing viral reactivation (R). Higher expressions of peripheral IL-1β, IL-23, and IL-12p40 in R children were observed. Lower expressions of local and peripheral TNF-α in patients with broader expressions of latent and lytic viral proteins were demonstrated. In healthy carrier (HC) patients, IL-23 positively correlated with CD163, and IP-10 positively correlated with CD68. Our results indicated that EBV might modulate antigen expression in the presence of TNF-α and influence peripheral cytokine expression differently in each stage of infection. Moreover, peripheral cytokines might have a particular role in macrophage polarization in HC. |
format | Online Article Text |
id | pubmed-10612087 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-106120872023-10-29 EBV Impact in Peripheral Macrophages’ Polarization Cytokines in Pediatric Patients Moyano, Agustina Ferressini Gerpe, Natalia Amarillo, Maria Eugenia De Matteo, Elena Preciado, Maria Victoria Caldirola, Maria Soledad Chabay, Paola Viruses Article Macrophages are exceptionally flexible cells. The presence of inflammatory cytokines such as IFN-γ and TNF-α results in an M1 (CD68) activation, while cytokines such as IL-10 or TGF-β induce the M2 (CD163) activation. Our aim was to study the behavior of peripheral cytokines involved in macrophage polarization and relate them with tissue findings to further comprehend the role of macrophages in EBV pediatric infection. We studied cytokine expression in tonsils and peripheral blood samples of children in different stages of infection. Peripheral cytokines were compared with macrophage polarization markers and viral protein expression in tonsils. Only IL-10 showed a negative correlation between compartments, exclusively in patients undergoing viral reactivation (R). Higher expressions of peripheral IL-1β, IL-23, and IL-12p40 in R children were observed. Lower expressions of local and peripheral TNF-α in patients with broader expressions of latent and lytic viral proteins were demonstrated. In healthy carrier (HC) patients, IL-23 positively correlated with CD163, and IP-10 positively correlated with CD68. Our results indicated that EBV might modulate antigen expression in the presence of TNF-α and influence peripheral cytokine expression differently in each stage of infection. Moreover, peripheral cytokines might have a particular role in macrophage polarization in HC. MDPI 2023-10-17 /pmc/articles/PMC10612087/ /pubmed/37896882 http://dx.doi.org/10.3390/v15102105 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Moyano, Agustina Ferressini Gerpe, Natalia Amarillo, Maria Eugenia De Matteo, Elena Preciado, Maria Victoria Caldirola, Maria Soledad Chabay, Paola EBV Impact in Peripheral Macrophages’ Polarization Cytokines in Pediatric Patients |
title | EBV Impact in Peripheral Macrophages’ Polarization Cytokines in Pediatric Patients |
title_full | EBV Impact in Peripheral Macrophages’ Polarization Cytokines in Pediatric Patients |
title_fullStr | EBV Impact in Peripheral Macrophages’ Polarization Cytokines in Pediatric Patients |
title_full_unstemmed | EBV Impact in Peripheral Macrophages’ Polarization Cytokines in Pediatric Patients |
title_short | EBV Impact in Peripheral Macrophages’ Polarization Cytokines in Pediatric Patients |
title_sort | ebv impact in peripheral macrophages’ polarization cytokines in pediatric patients |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10612087/ https://www.ncbi.nlm.nih.gov/pubmed/37896882 http://dx.doi.org/10.3390/v15102105 |
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