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EBV Impact in Peripheral Macrophages’ Polarization Cytokines in Pediatric Patients

Macrophages are exceptionally flexible cells. The presence of inflammatory cytokines such as IFN-γ and TNF-α results in an M1 (CD68) activation, while cytokines such as IL-10 or TGF-β induce the M2 (CD163) activation. Our aim was to study the behavior of peripheral cytokines involved in macrophage p...

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Autores principales: Moyano, Agustina, Ferressini Gerpe, Natalia, Amarillo, Maria Eugenia, De Matteo, Elena, Preciado, Maria Victoria, Caldirola, Maria Soledad, Chabay, Paola
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10612087/
https://www.ncbi.nlm.nih.gov/pubmed/37896882
http://dx.doi.org/10.3390/v15102105
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author Moyano, Agustina
Ferressini Gerpe, Natalia
Amarillo, Maria Eugenia
De Matteo, Elena
Preciado, Maria Victoria
Caldirola, Maria Soledad
Chabay, Paola
author_facet Moyano, Agustina
Ferressini Gerpe, Natalia
Amarillo, Maria Eugenia
De Matteo, Elena
Preciado, Maria Victoria
Caldirola, Maria Soledad
Chabay, Paola
author_sort Moyano, Agustina
collection PubMed
description Macrophages are exceptionally flexible cells. The presence of inflammatory cytokines such as IFN-γ and TNF-α results in an M1 (CD68) activation, while cytokines such as IL-10 or TGF-β induce the M2 (CD163) activation. Our aim was to study the behavior of peripheral cytokines involved in macrophage polarization and relate them with tissue findings to further comprehend the role of macrophages in EBV pediatric infection. We studied cytokine expression in tonsils and peripheral blood samples of children in different stages of infection. Peripheral cytokines were compared with macrophage polarization markers and viral protein expression in tonsils. Only IL-10 showed a negative correlation between compartments, exclusively in patients undergoing viral reactivation (R). Higher expressions of peripheral IL-1β, IL-23, and IL-12p40 in R children were observed. Lower expressions of local and peripheral TNF-α in patients with broader expressions of latent and lytic viral proteins were demonstrated. In healthy carrier (HC) patients, IL-23 positively correlated with CD163, and IP-10 positively correlated with CD68. Our results indicated that EBV might modulate antigen expression in the presence of TNF-α and influence peripheral cytokine expression differently in each stage of infection. Moreover, peripheral cytokines might have a particular role in macrophage polarization in HC.
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spelling pubmed-106120872023-10-29 EBV Impact in Peripheral Macrophages’ Polarization Cytokines in Pediatric Patients Moyano, Agustina Ferressini Gerpe, Natalia Amarillo, Maria Eugenia De Matteo, Elena Preciado, Maria Victoria Caldirola, Maria Soledad Chabay, Paola Viruses Article Macrophages are exceptionally flexible cells. The presence of inflammatory cytokines such as IFN-γ and TNF-α results in an M1 (CD68) activation, while cytokines such as IL-10 or TGF-β induce the M2 (CD163) activation. Our aim was to study the behavior of peripheral cytokines involved in macrophage polarization and relate them with tissue findings to further comprehend the role of macrophages in EBV pediatric infection. We studied cytokine expression in tonsils and peripheral blood samples of children in different stages of infection. Peripheral cytokines were compared with macrophage polarization markers and viral protein expression in tonsils. Only IL-10 showed a negative correlation between compartments, exclusively in patients undergoing viral reactivation (R). Higher expressions of peripheral IL-1β, IL-23, and IL-12p40 in R children were observed. Lower expressions of local and peripheral TNF-α in patients with broader expressions of latent and lytic viral proteins were demonstrated. In healthy carrier (HC) patients, IL-23 positively correlated with CD163, and IP-10 positively correlated with CD68. Our results indicated that EBV might modulate antigen expression in the presence of TNF-α and influence peripheral cytokine expression differently in each stage of infection. Moreover, peripheral cytokines might have a particular role in macrophage polarization in HC. MDPI 2023-10-17 /pmc/articles/PMC10612087/ /pubmed/37896882 http://dx.doi.org/10.3390/v15102105 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Moyano, Agustina
Ferressini Gerpe, Natalia
Amarillo, Maria Eugenia
De Matteo, Elena
Preciado, Maria Victoria
Caldirola, Maria Soledad
Chabay, Paola
EBV Impact in Peripheral Macrophages’ Polarization Cytokines in Pediatric Patients
title EBV Impact in Peripheral Macrophages’ Polarization Cytokines in Pediatric Patients
title_full EBV Impact in Peripheral Macrophages’ Polarization Cytokines in Pediatric Patients
title_fullStr EBV Impact in Peripheral Macrophages’ Polarization Cytokines in Pediatric Patients
title_full_unstemmed EBV Impact in Peripheral Macrophages’ Polarization Cytokines in Pediatric Patients
title_short EBV Impact in Peripheral Macrophages’ Polarization Cytokines in Pediatric Patients
title_sort ebv impact in peripheral macrophages’ polarization cytokines in pediatric patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10612087/
https://www.ncbi.nlm.nih.gov/pubmed/37896882
http://dx.doi.org/10.3390/v15102105
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