Computational assessment of visual coding across mouse brain areas and behavioural states

INTRODUCTION: Our brain is bombarded by a diverse range of visual stimuli, which are converted into corresponding neuronal responses and processed throughout the visual system. The neural activity patterns that result from these external stimuli vary depending on the object or scene being observed, but they also change as a result of internal or behavioural states. This raises the question of to what extent it is possible to predict the presented visual stimuli from neural activity across behavioural states, and how this varies in different brain regions. METHODS: To address this question, we assessed the computational capacity of decoders to extract visual information in awake behaving mice, by analysing publicly available standardised datasets from the Allen Brain Institute. We evaluated how natural movie frames can be distinguished based on the activity of units recorded in distinct brain regions and under different behavioural states. This analysis revealed the spectrum of visual information present in different brain regions in response to binary and multiclass classification tasks. RESULTS: Visual cortical areas showed highest classification accuracies, followed by thalamic and midbrain regions, with hippocampal regions showing close to chance accuracy. In addition, we found that behavioural variability led to a decrease in decoding accuracy, whereby large behavioural changes between train and test sessions reduced the classification performance of the decoders. A generalised linear model analysis suggested that this deterioration in classification might be due to an independent modulation of neural activity by stimulus and behaviour. Finally, we reconstructed the natural movie frames from optimal linear classifiers, and observed a strong similarity between reconstructed and actual movie frames. However, the similarity was significantly higher when the decoders were trained and tested on sessions with similar behavioural states. CONCLUSION: Our analysis provides a systematic assessment of visual coding in the mouse brain, and sheds light on the spectrum of visual information present across brain areas and behavioural states.